Initial adhesion of bone marrow stromal cells to various bone graft substitutes
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Purpose: The aim of this study is to determine whether certain biomaterials have the potential to support cell attachment. After seeding bone marrow stromal cells onto the biomaterials, we investigated their responses to each material in vitro. Methods: Rat bone marrow derived stromal cells were used. The biomaterials were deproteinized bovine bone mineral (DBBM), DBBM coated with fibronectin (FN), synthetic hydroxyapatite (HA), HA coated with FN, HA coated with β-tricalcium phosphate (TCP), and pure β-TCP. With confocal laser scanning microscopy, actin filaments and vinculin were observed after 6, 12, and 24 hours of cell seeding. The morphological features of cells on each biomaterial were observed using scanning electron microscopy at day 1 and 7. Results: The cells on HA/FN and HA spread widely and showed better defined actin cytoskeletons than those on the other biomaterials. At the initial phase, FN seemed to have a favorable effect on cell adhesion. In DBBM, very few cells adhered to the surface. Conclusions: Within the limitations of this study, we can conclude that in contrast with DBBM not supporting cell attachment, HA provided a more favorable environment with respect to cell attachment.
Abstract
Purpose: The aim of this study is to determine whether certain biomaterials have the potential to support cell attachment. After seeding bone marrow stromal cells onto the biomaterials, we investigated their responses to each material in vitro. Methods: Rat bone marrow derived stromal cells were used. The biomaterials were deproteinized bovine bone mineral (DBBM), DBBM coated with fibronectin (FN), synthetic hydroxyapatite (HA), HA coated with FN, HA coated with β-tricalcium phosphate (TCP), and pure β-TCP. With confocal laser scanning microscopy, actin filaments and vinculin were observed after 6, 12, and 24 hours of cell seeding. The morphological features of cells on each biomaterial were observed using scanning electron microscopy at day 1 and 7. Results: The cells on HA/FN and HA spread widely and showed better defined actin cytoskeletons than those on the other biomaterials. At the initial phase, FN seemed to have a favorable effect on cell adhesion. In DBBM, very few cells adhered to the surface. Conclusions: Within the limitations of this study, we can conclude that in contrast with DBBM not supporting cell attachment, HA provided a more favorable environment with respect to cell attachment.
참고문헌 (30)
- van den Dolder J, "Effect of fibronectin- and collagen I-coated titanium fiber mesh on proliferation and differentiation of osteogenic cells", Tissue Eng, 9, pp.505~515 (2003)
- Yoshimoto H, "A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering", Biomaterials, 24, pp.2077~2082 (2003)
- Woo KM, "Nano-fibrous scaffolding promotes osteoblast differentiation and biomineralization", Biomaterials, 28, pp.335~343 (2007)
- Langer R, "Tissue engineering", Science, 260, pp.920~926 (1993)
- Turhani D, "Invitro study of adherent mandibular osteoblast-like cells on carrier materials", Int J Oral Maxillofac Surg, 34, pp.543~550 (2005)
- Okumura A, "Substrate affects the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2)", Biomaterials, 22, pp.2263~2271 (2001)
- Ohgushi H, "Marrow cell induced osteogenesis in porous hydroxyapatite and tricalcium phosphate: a comparative histomorphometric study of ectopic bone formation", J Biomed Mater Res, 24, pp.1563~1570
- Kon E, "Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones", J Biomed Mater Res, pp.328~337 (2000)
- Hattori H, "Bone formation using human adipose tissue-derived stromal cells and a biodegradable scaffold", J Biomed Mater Res B Appl Biomater, 76, pp.230~239 (2006)
- Marino G, "Beta-tricalcium phosphate 3D scaffold promote alone osteogenic differentiation of human adipose stem cells: in vitro study", J Mater Sci Mater Med, 21, pp.353~363 (2010)
- Dong J, "Promotion of bone formation using highly pure porous beta-TCP combined with bone marrow-derived osteoprogenitor cells", Biomaterials, 23, pp.4493~4502 (2002)
- Boo JS, "Tissue-engineered bone using mesenchymal stem cells and a biodegradable scaffold", J Craniofac Surg, 13, pp.231~239 (2002)
- Camelo M, "Clinical, radiographic, and histologic evaluation of human periodontal defects treated with Bio-Oss and Bio-Gide", Int J Periodontics Restorative Dent, 18, pp.321~331 (1998)
- Stephan EB, "Anorganic bovine bone supports osteoblastic cell attachment and proliferation", J Periodontol, 70, pp.364~369 (1999)
- Piattelli M, "Bone reactions to anorganic bovine bone (Bio-Oss) used in sinus augmentation procedures: a histologic long-term report of 20 cases in humans", Int J Oral Maxillofac Implants, 14, pp.835~840 (1999)
- Grzesik WJ, "Bone matrix RGD glycoproteins: immunolocalization and interaction with human primary osteoblastic bone cells in vitro", J Bone Miner Res, 9, pp.487~496 (1994)
- Grzesik WJ, "Synthetic integrin-binding peptides promote adhesion and proliferation of human periodontal ligament cells in vitro", J Dent Res, 77, pp.1606~1612 (1998)
- Maniatopoulos C, "Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats", Cell Tissue Res, 254, pp.317~330 (1988)
- Anselme K, "Osteoblast adhesion on biomaterials", Biomaterials, 21, pp.667~681 (2000)
- Brodie JC, "Osteoblast interactions with calcium phosphate ceramics modified by coating with type I collagen", J Biomed Mater Res A, 73, pp.409~421 (2005)
- Petrovic L, "Different substitute biomaterials as potential scaffolds in tissue engineering", Int J Oral Maxillofac Implants, 21, pp.225~231 (2006)
- Herten M, "Surface- and nonsurface-dependent in vitro effects of bone substitutes on cell viability", Clin Oral Investig, 13, pp.149~155 (2009)
- Wiedmann-Al-Ahmad M, "Search for ideal biomaterials to cultivate human osteoblast-like cells for reconstructive surgery", J Mater Sci Mater Med, 16, pp.57~66 (2005)
- Kübler A, "Growth and proliferation of human osteoblasts on different bone graft substitutes: an in vitro study", Implant Dent, 13, pp.171~179 (2004)
- Açil Y, "Three-dimensional cultivation of human osteoblast-like cells on highly porous natural bone mineral", J Biomed Mater Res, 51, pp.703~710 (2000)
- Ignjatovic@ N, "Cytotoxicity and fibroblast properties during in vitro test of biphasic calcium phosphate", poly-dl-lactide-co-glycolide biocomposites and different phosphate materials, 2006, pp.69, 976~982
- Carmagnola D, "Healing of human extraction sockets filled with Bio-Oss", Clin Oral Implants Res, 14, pp.137~143 (2003)
- Zitzmann NU, "Alveolar ridge augmentation with Bio-Oss: a histologic study in humans", Int J Periodontics Restorative Dent, 21, pp.288~295 (2001)
- Burmeister JS, "Effect of fibronectin amount and conformation on the strength of endothelial cell adhesion to HEMA", EMA copolymers, 1996, pp.30, 13~22
- Suzuki T, "Growth and adhesion of osteoblast-like cells derived from neonatal rat calvaria on calcium phosphate ceramics", J Biosci Bioeng, 89, pp.18~26 (2000)