Antioxidant therapy enhances pulpal healing in bleached teeth
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objectives: The purpose of this study was to evaluate the histopathological effects of an antioxidant therapy on the pulp tissue of rat teeth exposed to a bleaching gel with 35% hydrogen peroxide. Materials and Methods: Forty rats were subjected to oral ingestion by gavage of distilled water (DW) or ascorbic acid (AA) 90 min before the bleaching therapy. For the bleaching treatment, the agent was applied twice for 5 min each to buccal surfaces of the first right mandibular molars. Then, the animals were sacrificed at 6 hr, 24 hr, 3 day, or 7 day post-bleaching, and the teeth were processed for microscopic evaluation of the pulp tissue. Results: At 6 hr, the pulp tissue showed moderate inflammatory reactions in all teeth of both groups. In the DW and AA groups, 100% and 80% of teeth exhibited pulp tissue with significant necrosis and intense tissue disorganization, respectively. At 24 hr, the AA-treated group demonstrated a greater regenerative capability than the DW group, with less intense inflammatory reaction and new odontoblast layer formation in 60% of the teeth. For up to the 7 day period, the areas of pulpal necrosis were replaced by viable connective tissue, and the dentin was underlined by differentiated odontoblast-like cells in most teeth of both groups. Conclusions: A slight reduction in initial pulpal damage during post-bleaching was promoted by AA therapy. However, the pulp tissue of AA-treated animals featured faster regenerative potential over time.
Abstract
Objectives: The purpose of this study was to evaluate the histopathological effects of an antioxidant therapy on the pulp tissue of rat teeth exposed to a bleaching gel with 35% hydrogen peroxide. Materials and Methods: Forty rats were subjected to oral ingestion by gavage of distilled water (DW) or ascorbic acid (AA) 90 min before the bleaching therapy. For the bleaching treatment, the agent was applied twice for 5 min each to buccal surfaces of the first right mandibular molars. Then, the animals were sacrificed at 6 hr, 24 hr, 3 day, or 7 day post-bleaching, and the teeth were processed for microscopic evaluation of the pulp tissue. Results: At 6 hr, the pulp tissue showed moderate inflammatory reactions in all teeth of both groups. In the DW and AA groups, 100% and 80% of teeth exhibited pulp tissue with significant necrosis and intense tissue disorganization, respectively. At 24 hr, the AA-treated group demonstrated a greater regenerative capability than the DW group, with less intense inflammatory reaction and new odontoblast layer formation in 60% of the teeth. For up to the 7 day period, the areas of pulpal necrosis were replaced by viable connective tissue, and the dentin was underlined by differentiated odontoblast-like cells in most teeth of both groups. Conclusions: A slight reduction in initial pulpal damage during post-bleaching was promoted by AA therapy. However, the pulp tissue of AA-treated animals featured faster regenerative potential over time.
참고문헌 (38)
- Cintra LT, "The number of bleaching sessions influences pulp tissue damage in rat teeth", J Endod, 39, pp.1576~1580 (2013)
- Soares DG, "Concentrations of and application protocols for hydrogen peroxide bleaching gels: effects on pulp cell viability and whitening efficacy", J Dent, 42, pp.185~198 (2014)
- Soares DG, "Effect of hydrogen-peroxide-mediated oxidative stress on human dental pulp cells", J Dent, 43, pp.750~756 (2015)
- Soares DG, "Indirect cytocompatibility of a low-concentration hydrogen peroxide bleaching gel to odontoblast-like cells", Int Endod J, 49, pp.26~36 (2016)
- de Almeida LC, "Occurrence of sensitivity during at-home and in-office tooth bleaching therapies with or without use of light sources", Acta Odontol Latinoam, 25, pp.3~8 (2012)
- Bonafe E, "Tooth sensitivity and efficacy of in-office bleaching in restored teeth", J Dent, 41, pp.363~369 (2013)
- Costa CA, "Human pulp responses to in-office tooth bleaching", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 109, pp.e59~e64 (2010)
- Paula E, "The effect of perioperative ibuprofen use on tooth sensitivity caused by in-office bleaching", Oper Dent, 38, pp.601~608 (2013)
- Soares DG, "Effect of fluoride-treated enamel on indirect cytotoxicity of a 16% carbamide peroxide bleaching gel to pulp cells", Braz Dent J, 24, pp.121~127 (2013)
- Reis A, "Assessment of tooth sensitivity using a desensitizer before light-activated bleaching", Oper Dent, 36, pp.12~17 (2011)
- Lima AF, "Protective effect of sodium ascorbate on MDPC-23odontoblast-like cells exposed to a bleaching agent", Eur J Dent, 4, pp.238~244 (2010)
- Lima AF, "Transdentinal protective role of sodium ascorbate against the cytopathic effects of H2O2released from bleaching agents", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 109, pp.e70~e76 (2010)
- Vargas Fda S, "Dose-response and time-course of α-tocoferol mediating the cytoprotection of dental pulp cells against hydrogen peroxide", Braz Dent J, 25, pp.367~371 (2014)
- de Paula EA, "Administration of ascorbic acid to prevent bleachinginduced tooth sensitivity: a randomized triple-blind clinical trial", Oper Dent, 39, pp.128~135 (2014)
- de Silveira Vargas F, "Protective effect of alphatocopherol isomer from vitamin E against the H2O2induced toxicity on dental pulp cells", Biomed Res Int, 2014, pp.895049~ (2014)
- Lima AF, "Effect of bleaching treatment and reduced application time of an antioxidant on bond strength to bleached enamel and subjacent dentin", J Adhes Dent, 13, pp.537~542 (2011)
- 왕효진, "Effects of applying antioxidants on bond strength of bleached bovine dentin", Restorative Dentistry and Endodontics, 40(1), pp.37~43 (2015), 대한치과보존학회[DOI]
- 박재영, "Effective application duration of sodium ascorbate antioxidant in reducing microleakage of bonded composite restoration in intracoronally-bleached teeth", Restorative Dentistry and Endodontics, 38, pp.43~47 (2013), 대한치과보존학회[DOI]
- Ekuni D, "Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress", Arch Oral Biol, 54, pp.495~502 (2009)
- Tomofuji T, "Effects of vitamin C intake on gingival oxidative stress in rat periodontitis", Free Radic Biol Med, 46, pp.163~168 (2009)
- Sanbe T, "Vitamin C intake inhibits serum lipid peroxidation and osteoclast differentiation on alveolar bone in rats fed on a high-cholesterol diet", Arch Oral Biol, 54, pp.235~240 (2009)
- Patra RC, "Antioxidant effects of alpha tocopherol, ascorbic acid and L-methionine on lead induced oxidative stress to the liver, kidney and brain in rats", Toxicology, 162, pp.81~88 (2001)
- Chandra Jagetia G, "Alteration in the glutathione, glutathione peroxidase, superoxide dismutase and lipid peroxidation by ascorbic acid in the skin of mice exposed to fractionated gamma radiation", Clin Chim Acta, 332, pp.111~121 (2003)
- Nandi D, "Effect of cysteine, methionine, ascorbic acid and thiamine on arsenicinduced oxidative stress and biochemical alterations in rats", Toxicology, 211, pp.26~35 (2005)
- Jagetia GC, "Evaluation of the effect of ascorbic acid treatment on wound healing in mice exposed to different doses of fractionated gamma radiation", Radiat Res, 159, pp.371~380 (2003)
- Kaplan B, "Relationships between tensile strength, ascorbic acid, hydroxyproline, and zinc levels of rabbit full-thickness incision wound healing", Surg Today, 34, pp.747~751 (2004)
- Kadirvel R, "Supplementation of ascorbic acid and alpha-tocopherol prevents arsenic-induced protein oxidation and DNA damage induced by arsenic in rats", Hum Exp Toxicol, 26, pp.939~946 (2007)
- Chandler NP., "The radiographic assessment of pulp size:validity and clinical implications", N Z Dent J, 85, pp.23~26 (1989)
- Kina JF, "Response of human pulps after professionally applied vital tooth bleaching", Int Endod J, 43, pp.572~580 (2010)
- Roderjan DA, "Histopathological features of dental pulp tissue from bleached mandibular incisors", J Mater Sci Eng B, 6, pp.178~185 (2014)
- Costa CA, "Biocompatibility of resin-based materials used as pulpcapping agents", Int Endod J, 36, pp.831~839 (2003)
- Accorinte Mde L, "Response of human pulp capped with a bonding agent after bleeding control with hemostatic agents", Oper Dent, 30, pp.147~155 (2005)
- de Souza Costa CA, "Methods to evaluate and strategies to improve the biocompatibility of dental materials and operative techniques", Dent Mater, 30, pp.769~784 (2014)
- Kadirvel R, "Supplementation of ascorbic acid and alpha-tocopherol prevents arsenic-induced protein oxidation and DNA damage induced by arsenic in rats", Hum Exp Toxicol, 26, pp.939~946 (2007)
- Moores J., "Vitamin C: a wound healing perspective", Br J Community Nurs, (S6), pp.S8~S11 (2013)
- Levine M, "Vitamin C: a concentration-function approach yields pharmacology and therapeutic discoveries", Adv Nutr, 2, pp.78~88 (2011)
- Padayatty SJ, "Vitamin C pharmacokinetics: implications for oral and intravenous use", Ann Intern Med, 140, pp.533~537 (2004)
- Schmalz G, "Pulp development, repair, and regeneration: challenges of the transition from traditional dentistry to biologically based therapies", J Endod, 40, pp.S2~S5 (2014)