Comparative evaluation of NovaMin desensitizer and Gluma desensitizer on dentinal tubule occlusion: a scanning electron microscopic study
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Purpose: In this study, the effect of calcium sodium phosphosilicate (NovaMin) desensitizing agent, which is a powder-basedsystem, and hydroxyethyl methacrylate and glutaraldehyde (Gluma desensitizer), which is liquid-based system, on dentinaltubule occlusion was analyzed by scanning electron microscope. The effects of the above two along with one control groupwere compared to determine the more effective method of sealing the dentinal tubules after initial application. Methods: Twenty specimens were allocated to each of 3 groups: Control, Gluma desensitizer, and NovaMin. Two additionalsamples were also prepared and treated with Gluma and NovaMin; these samples were longitudinally fractured. The specimenswere prepared from extracted sound human premolars and were stored in 10% formalin at room temperature. The teethwere cleaned of gross debris and then sectioned to provide one to two dentin specimens. The dentin specimens were etchedwith 6% citric acid for 2 minutes and rinsed in distilled water. Control discs were dried, and the test discs were treated withthe desensitizing agents as per the manufacturer’s instructions. The discs as well as longitudinal sections were later analyzedunder the scanning electron microscope. The proportions of completely occluded, partially occluded, and open tubules withineach group were calculated. The ratios of completely and partially occluded tubules to the total tubules for all the groups wasdetermined, and the data was statistically analyzed using nonparametric tests and statistical significance was calculated. Results: NovaMin showed more completely occluded tubules (0.545±0.051) while Gluma desensitizer showed more partiallyoccluded tubules (0.532±0.075). The differences among all the groups were statistically significant (P≤ 0.05). Conclusion: Both materials were effective in occluding dentinal tubules but NovaMin appeared more promising in occludingtubules completely after initial application.
Abstract
Purpose: In this study, the effect of calcium sodium phosphosilicate (NovaMin) desensitizing agent, which is a powder-basedsystem, and hydroxyethyl methacrylate and glutaraldehyde (Gluma desensitizer), which is liquid-based system, on dentinaltubule occlusion was analyzed by scanning electron microscope. The effects of the above two along with one control groupwere compared to determine the more effective method of sealing the dentinal tubules after initial application. Methods: Twenty specimens were allocated to each of 3 groups: Control, Gluma desensitizer, and NovaMin. Two additionalsamples were also prepared and treated with Gluma and NovaMin; these samples were longitudinally fractured. The specimenswere prepared from extracted sound human premolars and were stored in 10% formalin at room temperature. The teethwere cleaned of gross debris and then sectioned to provide one to two dentin specimens. The dentin specimens were etchedwith 6% citric acid for 2 minutes and rinsed in distilled water. Control discs were dried, and the test discs were treated withthe desensitizing agents as per the manufacturer’s instructions. The discs as well as longitudinal sections were later analyzedunder the scanning electron microscope. The proportions of completely occluded, partially occluded, and open tubules withineach group were calculated. The ratios of completely and partially occluded tubules to the total tubules for all the groups wasdetermined, and the data was statistically analyzed using nonparametric tests and statistical significance was calculated. Results: NovaMin showed more completely occluded tubules (0.545±0.051) while Gluma desensitizer showed more partiallyoccluded tubules (0.532±0.075). The differences among all the groups were statistically significant (P≤ 0.05). Conclusion: Both materials were effective in occluding dentinal tubules but NovaMin appeared more promising in occludingtubules completely after initial application.
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