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상아모세포 관련 유전자, OD314의 발현과 기능 연구

Expression and functional characterization of odontoblast-derived gene:OD314

김흥중|박주철|정문진|손호현|김두현
대한치과보존학회 (Korean Academy Of Conservative Dentistry)|2004.07|Restorative Dentistry and Endodontics|제29권 제4호|pp. 399-408

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참고문헌

주제분류치의학

초록

Abstract

Odontoblasts are responsible for the formation and maintenance of dentin. They are known to synthesize unique gene products including dentin sialophosphoprotein (DSPP). Another unique genes of the cells remain unclear. OD314 was isolated from the odontoblasts/pulp cells of rats and partially characterized as an odontoblast-enriched gene (Dey et al., 2001). This study aimed to elucidate the biological function of OD314, relating to odontoblast differentiation and dentinogenesis. After determining the open reading frame (ORF) of OD314 by transient transfection analysis using green fluorescent protein (GFP) expression vector, mRNA in-situ hybridization, immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and western analysis were performed. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were expressed in odontoblasts of developing coronal and root pulp. 2. OD314 was a novel protein encoding 154 amino acids, and the protein was mainly expressed in cytoplasm by transient transfection analysis. 3. Mineralized nodules were associated with multilayer cell nodules in the culture of human dental pulp cells and first detected from day 21 using alizarin-red S staining. 4. In RT-PCR analysis, OD314, osteocalcin (OC) and DSPP strongly expressed throughout 28 days of culture. Whereas, osteonectin (ON) mRNA expression stayed low up to day 14, and then gradually decreased from day 21. 5. Western blots showed an approximately 17 kDa band. OD314 protein was expressed from the start of culture and then increased greatly from day 21. In conclusion, OD314 is considered as an odontoblast-enriched gene and may play important roles in odontoblast differentiation and dentin mineralization.

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관련 논문AI추천

흰쥐 상아질모세포 분화과정에서 OD314의 역할김익환, 김흥중, 박주철, 손호현, 오현주, 정문진, 정제오대한체질인류학회 · 해부·생물인류학2005 · 제18권 제3호골모세포주，상아모세포주 및 백악모세포주에서OD314의 분포에 관한 면역세포화학적 연구Ho-Hyun Son, Hyun Ju Oh, In-Cheol Shin, Je-O Jeong, JOO-CHEOL PARK, Min-Ju Park, MOON-JIN JEONG, Sung Min Ahn대한구강해부학회 · 대한구강해부학회지2005 · 제29권 제1호RNA interference를 이용한 OD314 유전자의 발현억제가 상아질모세포 전구세포에 미치는 영향김흥중, 박주철, 손호현, 정문지대한체질인류학회 · 해부·생물인류학2004 · 제17권 제2호법랑모세포 분화와 성숙과정에서 OD314의 발현김흥중, 박민주, 박주철, 손호현, 신인철, 안성민, 정문진대한치과보존학회 · Restorative Dentistry and Endodontics2005 · 제30권 제5호법랑모세포 분화와 법랑질 형성과정에서 OD314, Apin protein의 발현 및 기능김흥중, 박종태, 박주철, 손호현, 신인철, 오현주, 정문진, 최용석대한치과보존학회 · Restorative Dentistry and Endodontics2006 · 제31권 제6호상아모세포의 조건배지를 이용한 백악모세포의 분화와 석회화 조절김혜선, 김흥중, 문상원, 박종태, 박주철, 송현정, 장현선, 최홍규대한치주과학회 · Journal of Periodontal & Implant Science2006 · 제36권 제2호

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