MTA와 포틀랜드 시멘트의 구성성분분석과 세포독성에 관한 연구
Ingredients and cytotoxicity of MTA and 3 kinds of Portland cements
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The aim of this study was to compare the compositions and cytotoxicity of white ProRoot MTA (white mineral trioxide aggregate) and 3 kinds of Portland cements. The elements, simple oxides and phase compositions of white MTA (WMTA), gray Portland cement (GPC), white Portland cement (WPC) and fast setting cement (FSC) were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES), X-ray fluorescence spectrometry (XRF) and X-ray diffractometry (XRD). Agar diffusion test was carried out to evaluate the cytotoxicity of WMTA and 3 kinds of Portland cements. The results showed that WMTA and WPC contained far less magnesium (Mg), iron (Fe), manganese (Mn), and zinc (Zn) than GPC and FSC. FSC contained far more aluminum oxide (Al₂O₃) than WMTA, GPC, and WPC. WMTA, GPC, WPC and FSC were composed of main phases, such as tricalcicium silicate (3CaO∙SiO₂), dicalcium silicate (2CaO∙SiO₂), tricalcium aluminate (3CaO∙Al₂O₃), and tetracalcium aluminoferrite (4CaO∙Al₂O₃∙Fe₂O₄). The significance of the differences in cellular response between WMTA, GPC, WPC and FSC was statistically analyzed by Kruskal-Wallis Exact test with Bonferroni’s correction. The result showed no statistically significant difference (p > 0.05). WMTA, GPC, WPC and FSC showed similar compositions. However there were notable differences in the content of minor elements, such as aluminum (Al), magnesium, iron, manganese, and zinc. These differences might influence the physical properties of cements.
Abstract
The aim of this study was to compare the compositions and cytotoxicity of white ProRoot MTA (white mineral trioxide aggregate) and 3 kinds of Portland cements. The elements, simple oxides and phase compositions of white MTA (WMTA), gray Portland cement (GPC), white Portland cement (WPC) and fast setting cement (FSC) were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES), X-ray fluorescence spectrometry (XRF) and X-ray diffractometry (XRD). Agar diffusion test was carried out to evaluate the cytotoxicity of WMTA and 3 kinds of Portland cements. The results showed that WMTA and WPC contained far less magnesium (Mg), iron (Fe), manganese (Mn), and zinc (Zn) than GPC and FSC. FSC contained far more aluminum oxide (Al₂O₃) than WMTA, GPC, and WPC. WMTA, GPC, WPC and FSC were composed of main phases, such as tricalcicium silicate (3CaO∙SiO₂), dicalcium silicate (2CaO∙SiO₂), tricalcium aluminate (3CaO∙Al₂O₃), and tetracalcium aluminoferrite (4CaO∙Al₂O₃∙Fe₂O₄). The significance of the differences in cellular response between WMTA, GPC, WPC and FSC was statistically analyzed by Kruskal-Wallis Exact test with Bonferroni’s correction. The result showed no statistically significant difference (p > 0.05). WMTA, GPC, WPC and FSC showed similar compositions. However there were notable differences in the content of minor elements, such as aluminum (Al), magnesium, iron, manganese, and zinc. These differences might influence the physical properties of cements.
참고문헌 (29)
- Torabinejad M, "Tissue reaction to implanted root-end filling materials in the tibia and mandible of guinea pigs", J Endod, 24, pp.468~471 (1998)
- Koh ET, "Mineral trioxide aggregate stimulates a biological response in human osteoblasts", J Biomed Mater Res, 37, pp.432~439 (1997)
- Al-Hezaimi K, "Antibacterial effect of two mineral trioxide aggregate (MTA) preparations against Enterococcus faecalis and Streptococcus sanguis in vitro", J Endod, 32, pp.1053~1056 (2006)
- Torabinejad M, "Sealing ability of a mineral trioxide aggregate when used as a root end filling material", J Endod, 19, pp.591~595 (1993)
- Torabinejad M, "Bacterial leakage of mineral trioxide aggregate as a root-end filling material", J Endod, 21, pp.109~112 (1995)
- Torabinejad M, "Comparative investigation of marginal adaptation of mineral trioxide aggregate and other commonly used root-end filling materials", J Endod, 21, pp.295~299 (1995)
- Torabinejad M, "Clinical applications of mineral trioxide aggregate", J Endod, 25, pp.197~205 (1999)
- Lee SJ, "Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations", J Endod, 19, pp.541~544 (1993)
- Pitt Ford TR, "Use of mineral trioxide aggregate for repair of furcal perforations", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 79, pp.756~763 (1995)
- Tselnik M, "Bacterial leakage with mineral trioxide aggregate or a resinmodified glass ionomer used as a coronal barrier", J Endod, 30, pp.782~784 (2004)
- Song JS, "Chemical analysis of powder and set forms of Portland cement, gray ProRoot MTA, white ProRoot MTA, and gray MTA-Angelus", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 102, pp.809~815 (2006)
- Islam I, "X-ray diffraction analysis of mineral trioxide aggregate and Portland cement", Int Endod J, 39, pp.220~225 (2006)
- Camilleri J, "The chemical constitution and biocompatibility of accelerated Portland cement for endodontic use", Int Endod J, 38, pp.832~842 (2005)
- De Deus G, "Cytotoxicity of MTA and Portland cement on human ECV 304 endothelial cells", Int Endod J, 38, pp.604~609 (2005)
- Ribeiro DA, "Biocompatibility in vitro tests of mineral trioxide aggregate and regular and white Portland cements", J Endod, 31, pp.605~607 (2005)
- de Morais CA, "Evaluation of tissue response to MTA and Portland cement with iodoform", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 102, pp.417~421 (2006)
- Santos AD, "Physico-chemical properties of MTA and a novel experimental cement", Int Endod J, 38, pp.443~447 (2005)
- Islam I, "Comparison of the physical and mechanical properties of MTA and portland cement", J Endod, 32, pp.193~197 (2006)
- De-Deus G, "MTA versus Portland cement as repair material for furcal perforations: a laboratory study using a polymicrobial leakage model", Int Endod J, 39, pp.293~298 (2006)
- Dammaschke T, "Chemical and physical surface and bulk material characterization of white ProRoot MTA and two Portland cements", Dent Mater, 21, pp.731~738 (2005)
- Torabinejad M, "Physical and chemical properties of a new root-end filling material", J Endod, 21, pp.349~353 (1995)
- Antunes Bortoluzzi E, "Monteiro Bramante C. The use of a setting accelerator and its effect on pH and calcium ion release of mineral trioxide aggregate and white Portland cement", J Endod, 32, pp.1194~1197 (2006)
- Bortoluzzi EA, "Sealing ability of MTA and radiopaque Portland cement with or without calcium chloride for root-end filling", J Endod, 32, pp.897~900 (2006)
- Tay FR, "Calcium Phosphate Phase Transformation Produced by the Interaction of the Portland Cement Component of White Mineral Trioxide Aggregate with a Phosphate-containing Fluid", J Endod, 33, pp.1347~1351 (2007)
- Camilleri J, "The constitution of mineral trioxide aggregate", Dent Mater, 21, pp.297~303 (2005)
- Camilleri J, "Characterization of Portland cement for use as a dental restorative material", Dent Mater, 22, pp.569~575 (2006)
- Coomaraswamy KS, "Effect of bismuth oxide radioopacifier content on the material properties of an endodontic Portland cement-based(MTA-like) system", J Endod, 33, pp.295~298 (2007)
- Camilleri J, "Hydration mechanisms of mineral trioxide aggregate", Int Endod J, 40, pp.462~470 (2007)
- Min KS, "Human pulp cells response to Portland cement in vitro", J Endod, 33, pp.163~166 (2007)