MMP-1 promoter polymorphism in Korean with generalized aggressive periodontitis
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Purpose: The aim of this study was to investigate matrix metalloproteinase 1 (MMP-1) gene polymorphism (1G/2G at -1607 and A/G at -519) in Korean subject and to assess the association between polymorphism and periodontal status. Methods: Forty nine generalized aggressive periodontitis (GAP) patients and 57 periodontally healthy children were recruited and genomic DNA was extracted from buccal swab. The polymorphisms of MMP-1 promoter genes were determined by polymerase chain reaction and restriction fragment length product (PCR-RFLP) method. The distribution of genotype and allele frequency was compared between 2 groups by χ² test. Results: There was a significant difference in the distribution of genotypes and frequency of alleles between the GAP and reference groups at the position -519 of MMP-1 gene promoter (P <0.05). Allele G carrier rate was significantly lower in GAP group than that of the reference group (P <0.001). At the position -1607 of MMP-1 gene promoter, genotype distribution and allele frequency showed no statistically significant difference between the groups. However, in the female group, a significant difference was observed between the groups for the genotype distribution, allele frequency and allele 1G carrier rate (P <0.05). Conclusions: The DNA polymorphism at the MMP-1 gene promoter might be associated with GAP in Korean.(J Korean Acad Periodontol 2009;39:269-278)
Abstract
Purpose: The aim of this study was to investigate matrix metalloproteinase 1 (MMP-1) gene polymorphism (1G/2G at -1607 and A/G at -519) in Korean subject and to assess the association between polymorphism and periodontal status. Methods: Forty nine generalized aggressive periodontitis (GAP) patients and 57 periodontally healthy children were recruited and genomic DNA was extracted from buccal swab. The polymorphisms of MMP-1 promoter genes were determined by polymerase chain reaction and restriction fragment length product (PCR-RFLP) method. The distribution of genotype and allele frequency was compared between 2 groups by χ² test. Results: There was a significant difference in the distribution of genotypes and frequency of alleles between the GAP and reference groups at the position -519 of MMP-1 gene promoter (P <0.05). Allele G carrier rate was significantly lower in GAP group than that of the reference group (P <0.001). At the position -1607 of MMP-1 gene promoter, genotype distribution and allele frequency showed no statistically significant difference between the groups. However, in the female group, a significant difference was observed between the groups for the genotype distribution, allele frequency and allele 1G carrier rate (P <0.05). Conclusions: The DNA polymorphism at the MMP-1 gene promoter might be associated with GAP in Korean.(J Korean Acad Periodontol 2009;39:269-278)
참고문헌 (36)
- Genco RJ, "Host responses in periodontal diseases: current concepts", J Periodontol, 63, pp.338~355 (1992)
- Haffajee AD, "Microbial etiological agents of destructive periodontal diseases", Periodontol 2000, 5, pp.78~111 (1994)
- Michalowicz BS, "Periodontal findings in adult twins", J Periodontol, 62, pp.293~299 (1991)
- Clark IM, "The regulation of matrix metalloproteinases and their inhibitors", Int J Biochem Cell Biol, 40, pp.1362~1378 (2008)
- Mäkelä M, "Matrix metalloproteinases (MMP-2 and MMP-9) of the oral cavity: cellular origin and relationship to periodontal status", J Dent Res, 73, pp.1397~1406 (1994)
- Birkedal-Hansen H, "Role of cytokines and inflammatory mediators in tissue destruction", J Periodontal Res, 28, pp.500~510 (1993)
- Kubota T, "Expression of mRNA for matrix metalloproteinases and tissue inhibitors of metalloproteinases in periodontitis-affected human gingival tissue", Arch Oral Biol, 41, pp.253~262 (1996)
- Séguier S, "Is collagen breakdown during periodontitis linked to inflammatory cells and expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival tissue?", J Periodontol, 72, pp.1398~1406
- Soell M, "Matrix metalloproteinases, and their tissue inhibitors in gingiva and gingival crevicular fluid from periodontitis-affected patients", J Dent Res, 81, pp.174~178 (2002)
- Kornman KS, "The interleukin-genotype as a severity factor in adult periodontal disease", J Clin Periodontol, 24, pp.72~77 (1997)
- 정하나, "한국인에서 치주질환과 관상동맥질환의 관련성에 대한 염증표지자와 IL-1 유전자 다변성의 영향", The Journal of the Korean Academy of Periodontology (JPIS), 34(3), pp.607~622 (2004), 대한치주과학회
- 방선정, "한국인 전반적 급진성 치주염 환자에서 IL-6 유전자 다변성에 관한 연구", The Journal of the Korean Academy of Periodontology (JPIS), 38(4), pp.579~588 (2008), 대한치주과학회
- 류지선, "한국인 전반적 급진성 치주염 환자에서 IL-10 promoter 유전자 다변성에 관한 연구", The Journal of the Korean Academy of Periodontology (JPIS), 37(3), pp.563~573 (2007), 대한치주과학회
- Rutter JL, "A single nucleotide polymorphism in the matrix metalloproteinase-1 promoter creates an Ets binding site and augments transcription", Cancer Res, 58, pp.5321~5325 (1998)
- Jurajda M, "A newly identifiednucleotide polymorphism in the promoter of the matrix metalloproteinase-1 gene", Mol Cell Probes, 16, pp.63~66 (2002)
- Hinoda Y, "Association of functional polymorphisms of matrix metalloproteinase (MMP)-1 and MMP-3 genescolorectal cancer", Int J Cancer, 102, pp.526~529 (2002)
- Ghilardi G, "Matrix metalloproteinase- 1 and matrix metalloproteinase-3 gene promoterpolymorphisms are associated with carotid artery stenosis", Stroke, 33, pp.2408~2412 (2002)
- Terashima M, "Stromelysin promoter 5A", 6A polymorphism is associated with acute myocardial infarction, 1999, pp.99, 2717~2719
- Kanamori Y, "Correlation between expression of the matrix metalloproteinase-1 gene in ovarian cancers and an insertion", deletion polymorphism in its promoter region, 1999, pp.59, 4225~4227
- de Souza AP, "MMP-1 promoter polymorphism: association with chronic periodontitis severity in a Brazilian population", J Clin Periodontol, 30, pp.154~158 (2003)
- Santos MC, "Analysis of MMP-1 and MMP-9 promoter polymorphisms in early osseointegrated implant failure", Int J Oral Maxillofac Implants, 19, pp.38~43 (2004)
- Hollá LI, "Genetic variations in the matrix metalloproteinase-1 promoter and risk of susceptibility and or severity of chronic periodontitis in the Czech population", J Clin Periodontol, 31, pp.685~690 (2004)
- Itagaki M, "Matrix metalloproteinase- 1 and -3 gene promoter polymorphisms in Japanese patients with periodontitis", J Clin Periodontol, 31, pp.764~769 (2004)
- Armitage GC, "Development of a classification system for periodontal diseases and conditions", Ann Periodontol, 4, pp.1~6 (1999)
- Trevilatto PC, "Use of buccal epithelial cells for PCR amplification of large DNA fragments", J Forensic Odontostomatol, 18, pp.6~9 (2000)
- Matrisian LM, "Metalloproteinase and their inhibitors in matrix remodelling", Trends in Genetics, 6, pp.121~125 (1990)
- 류상호, "단순 만성 치주염 환자와 제 2형 당뇨병을 동반한 만성 치주염 환자에서 Stromelysin-1와 Membrane type-MMP-1 Expressions", The Journal of the Korean Academy of Periodontology (JPIS), 38(4), pp.629~638 (2008), 대한치주과학회
- Ye S, "Polymorphism in matrix metalloproteinase gene promoters: implication in regulation of gene expression and susceptibility of various diseases", Matrix Biology, 19, pp.623~629 (2000)
- Astolfi CM, "Genetic polymorphisms in the MMP-1 and MMP-3 gene may contribute to chronic periodontitis in a Brazilian population", J Clin Periodontol, 33, pp.699~703 (2006)
- Cao Z, "Association of matrix metalloproteinase- 1 promoter polymorphism with generalized aggressive periodontitis in a Chinese population", J Periodontal Res, 40, pp.427~431 (2005)
- Ju W, "Promoter polymorphism in the matrix metalloproteinase-1 and risk of cervical cancer in Korean women", Cancer Lett, 20, pp.191~196 (2005)
- Nho YK, "Matrix metalloproteinase-1 promoter is associated with body mass index in Korean population with aged greater or equal to 50 years", Clin Chim Acta, 396, pp.14~17 (2008)
- Sternlicht MD, "How matrix metalloproteinases regulate cell behavior", Annu Rev Cell Dev Biol, 17, pp.463~516 (2001)
- Zhou Z, "Impaired endochondral ossification and angiogenesis in mice deficient in membrane- type matrix metalloproteinase I", Proc Natl Acad Sci USA, 97, pp.4052~4057 (2000)
- Pirhan D, "Effect of MMP-1 promoter polymorphisms on GCF MMP-1 levels andoutcome of periodontal therapy in patients with severe chronic periodontitis", J Clin Periodontol, 35, pp.862~870 (2008)
- Cao Z, "MMP-1 promoter gene polymorphism and susceptibility to chronic periodontitis in a Chinese population", Tissue Antigens, 68, pp.38~43 (2006)