Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model
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Purpose: The aim of this study was to assess the possible paracrine effect of bone morphogeneticprotein-2 (BMP-2) at the experimental site on the adjacent control site for validating a rabbit calvarial defect model as a means of verifying the effect of BMP-2. Methods: Sixteen rabbits were divided into two groups (n=8 in each) according to whether or not BMP-2 would be used. Two circular defects (8 mm in diameter) were created side by side, 2 mm apart, in the calvarium of all of the rabbits. In each animal, one of the defects was grafted with either BMP-2-loaded carrier or carrier material alone. The control defects adjacent to these grafted defects, designated CB (the nongrafted defect adjacent BMP-2-loaded carrier-grafted defect) and CC (the nongrafted defect adjacent to carrier only-grafted defect), respectively, were the focus of this study, and were filled only with a blood clot in all of the animals. Histologic observation and histomorphometric analysis were performed at 2 and 8 weeks (n=4 animals per point in time) after surgery. Results: There was no noteworthy difference in the healing pattern, and no statistically significant differences in histomorphometric parameters such as the defect closure, new bone area, or total augmented area between the CC and CB groups. Conclusions: The results of this study suggest that rabbit calvarial defects separated by a distance of 2 mm are suitable for evaluating the effects of BMP-2 and the control defect can be regarded not to be affected by BMP-2 applied defect.
Abstract
Purpose: The aim of this study was to assess the possible paracrine effect of bone morphogeneticprotein-2 (BMP-2) at the experimental site on the adjacent control site for validating a rabbit calvarial defect model as a means of verifying the effect of BMP-2. Methods: Sixteen rabbits were divided into two groups (n=8 in each) according to whether or not BMP-2 would be used. Two circular defects (8 mm in diameter) were created side by side, 2 mm apart, in the calvarium of all of the rabbits. In each animal, one of the defects was grafted with either BMP-2-loaded carrier or carrier material alone. The control defects adjacent to these grafted defects, designated CB (the nongrafted defect adjacent BMP-2-loaded carrier-grafted defect) and CC (the nongrafted defect adjacent to carrier only-grafted defect), respectively, were the focus of this study, and were filled only with a blood clot in all of the animals. Histologic observation and histomorphometric analysis were performed at 2 and 8 weeks (n=4 animals per point in time) after surgery. Results: There was no noteworthy difference in the healing pattern, and no statistically significant differences in histomorphometric parameters such as the defect closure, new bone area, or total augmented area between the CC and CB groups. Conclusions: The results of this study suggest that rabbit calvarial defects separated by a distance of 2 mm are suitable for evaluating the effects of BMP-2 and the control defect can be regarded not to be affected by BMP-2 applied defect.
참고문헌 (20)
- Kim JW, "Bone formation of block and particulated biphasic calcium phosphate lyophilized with Escherichia coli-derived recombinant human bone morphogenetic protein 2 in rat calvarial defects", ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 112(3), pp.298~306 (2011), MOSBY-ELSEVIER
- Kim JW, "Volumetric bone regenerative efficacy of biphasic calcium phosphate-collagen composite block loaded with rhBMP-2 in vertical bone augmentation model of a rabbit calvarium", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 100A(12), pp.3304~3313 (2012), WILEY-LISS DIV JOHN WILEY & SONS INC 111 RIVER ST HOBOKEN AMERICA NJ 07030
- Kuhn LT, "Fibroblast growth factor-2 and bone morphogenetic protein-2 have a synergistic stimulatory effect on bone formation in cell cultures from elderly mouse and human bone", J Gerontol A Biol Sci Med Sci, 68, pp.1170~1180 (2013)
- Li P, "Synergistic and sequential effects of BMP-2, bFGF and VEGF on osteogenic differentiation of rat osteoblasts", J Bone Miner Metab (2013)
- de Freitas RM, "Alveolar ridge and maxillary sinus augmentation using rhBMP-2: a systematic review", Clin Implant Dent Relat Res (2013)
- Hwang JW, "Comparative evaluation of three calcium phosphate synthetic block bone graft materials for bone regeneration in rabbit calvaria", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 100(8), pp.2044~2052 (2012), WILEY-LISS
- Lim HC, "Osteoconductive effects of calcium phosphate glass cement grafts in rabbit calvarial defects", Journal Of Biomedical Materials Research Part B-Applied Biomaterials, 95(1), pp.47~52 (2010)
- 손주연, "Spontaneous healing capacity of rabbit cranial defects of various sizes", Journal of Periodontal & Implant Science, 40(4), pp.180~187 (2010), 대한치주과학회
- Guda T, "Methods to analyze bone regenerative response to different rhBMP-2 doses in rabbit craniofacial defects", Tissue Eng Part C Methods (2014)
- Ahn SH, "Effect of recombinant human bone morphogenetic protein-4 with carriers in rat calvarial defects", J Periodontol, 74, pp.787~797 (2003)
- Pang EK, "Effect of recombinant human bone morphogenetic protein-4 dose on bone formation in a rat calvarial defect model", J Periodontol, 75, pp.1364~1370 (2004)
- Gilsanz V, "Effect of sex steroids on peak bone density of growing rabbits", Am J Physiol, 255(4 Pt 1), pp.E416~E421 (1988)
- Castaneda S, "Bone mineral measurements of subchondral and trabecular bone in healthy and osteoporotic rabbits", Skeletal Radiol, 35, pp.34~41 (2006)
- Caplanis N, "Effect of allogeneic freeze-dried demineralized bone matrix on regeneration of alveolar bone and periodontal attachment in dogs", J Clin Periodontol, 25, pp.801~806 (1998)
- Schmitz JP, "The critical size defect as an experimental model for craniomandibulofacial nonunions", Clin Orthop Relat Res, (205), pp.299~308 (1986)
- Hassanein AH, "Experimental comparison of cranial particulate bone graft, rhBMP-2, and split cranial bone graft for inlay cranioplasty", Cleft Palate Craniofac J, 50, pp.358~362 (2013)
- Schmidlin PR, "Evaluation of moldable, in situ hardening calcium phosphate bone graft substitutes", Clin Oral Implants Res, 24, pp.149~157 (2013)
- Jiang ZQ, "Repair of calvarial defects in rabbits with platelet-rich plasma as the scaffold for carrying bone marrow stromal cells", Oral Surg Oral Med Oral Pathol Oral Radiol, 113, pp.327~333 (2012)
- Tannoury CA, "Complications with the use of bone morphogenetic protein 2 (BMP-2) in spine surgery", Spine J, 14, pp.552~559 (2014)
- Deutsch H, "High-dose bone morphogenetic protein-induced ectopic abdomen bone growth", Spine J, 10, pp.e1~e4 (2010)