Differential expression of microRNAs in the saliva of patients with aggressive periodontitis: a pilot study of potential biomarkers for aggressive periodontitis
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Purpose: The aim of this study was to compare microRNA (miRNA) gene expression in saliva using miRNA polymerase chain reaction (PCR) arrays in healthy and aggressive periodontitis (AP) patients. Methods: PCR arrays of 84 miRNAs related to the human inflammatory response and autoimmunity from the saliva samples of 4 patients with AP and 4 healthy controls were performed. The functions and diseases related to the miRNAs were obtained using TAM 2.0. Experimentally validated targets of differentially expressed miRNAs were obtained from mirTarBase. Gene ontology terms and pathways were analyzed using ConsensusPathDB. Results: Four downregulated miRNAs (hsa-let-7a-5p, hsa-let-7f-5p, hsa-miR-181b-5p, and hsa-miR-23b-3p) were identified in patients with AP. These miRNAs are associated with cell death and innate immunity, and they target genes associated with osteoclast development and function. Conclusions: This study is the first analysis of miRNAs in the saliva of patients with AP. Identifying discriminatory human salivary miRNA biomarkers reflective of periodontal disease in a non-invasive screening assay is crucial for the development of salivary diagnostics. These data provide a first step towards the discovery of key salivary miRNA biomarkers for AP.
Abstract
Purpose: The aim of this study was to compare microRNA (miRNA) gene expression in saliva using miRNA polymerase chain reaction (PCR) arrays in healthy and aggressive periodontitis (AP) patients. Methods: PCR arrays of 84 miRNAs related to the human inflammatory response and autoimmunity from the saliva samples of 4 patients with AP and 4 healthy controls were performed. The functions and diseases related to the miRNAs were obtained using TAM 2.0. Experimentally validated targets of differentially expressed miRNAs were obtained from mirTarBase. Gene ontology terms and pathways were analyzed using ConsensusPathDB. Results: Four downregulated miRNAs (hsa-let-7a-5p, hsa-let-7f-5p, hsa-miR-181b-5p, and hsa-miR-23b-3p) were identified in patients with AP. These miRNAs are associated with cell death and innate immunity, and they target genes associated with osteoclast development and function. Conclusions: This study is the first analysis of miRNAs in the saliva of patients with AP. Identifying discriminatory human salivary miRNA biomarkers reflective of periodontal disease in a non-invasive screening assay is crucial for the development of salivary diagnostics. These data provide a first step towards the discovery of key salivary miRNA biomarkers for AP.
참고문헌 (38)
- Huntzinger E, "Gene silencing by microRNAs : contributions of translational repression and mRNA decay", Nat Rev Genet, 12, pp.99~110 (2011)
- Pasquinelli AE, "MicroRNAs and their targets: recognition, regulation and an emerging reciprocal relationship", Nat Rev Genet, 13, pp.271~282 (2012)
- Bartel DP, "MicroRNAs: genomics, biogenesis, mechanism, and function", Cell, 116, pp.281~297 (2004)
- Selbach M, "Widespread changes in protein synthesis induced by microRNAs", Nature, 455, pp.58~63 (2008)
- Irwandi RA, "The role of microRNA in periodontal tissue : a review of the literature", Arch Oral Biol, 72, pp.66~74 (2016)
- Kim SH, "MicroRNAs as biomarkers for dental diseases", Singapore Dent J, 36, pp.18~22 (2015)
- Eulalio A, "The mammalian microRNA response to bacterial infections", RNA Biol, 9, pp.742~750 (2012)
- Pauley KM, "MicroRNA in autoimmunity and autoimmune diseases", J Autoimmun, 32, pp.189~194 (2009)
- Alexander M, "Noncoding RNAs and chronic inflammation : micro-managing the fire within", BioEssays, 37, pp.1005~1015 (2015)
- Romaine SP, "MicroRNAs in cardiovascular disease : an introduction for clinicians", Heart, 101, pp.921~928 (2015)
- Peng Y, "The role of MicroRNAs in human cancer", Signal Transduct Target Ther, 1, pp.15004~ (2016)
- Luan X, "MicroRNAs and immunity in periodontal health and disease", Int J Oral Sci, 10, pp.24~ (2018)
- Wang J, "MicroRNA as biomarkers and diagnostics", J Cell Physiol, 231, pp.25~30 (2016)
- Ai J, "Circulating microRNA-1 as a potential novel biomarker for acute myocardial infarction", Biochem Biophys Res Commun, 391, pp.73~77 (2010)
- Mitchell PS, "Circulating microRNAs as stable blood-based markers for cancer detection", Proc Natl Acad Sci U S A, 105, pp.10513~10518 (2008)
- Xie Z, "Salivary microRNAs show potential as a noninvasive biomarker for detecting resectable pancreatic cancer", Cancer Prev Res(Phila), 8, pp.165~173 (2015)
- Park NJ, "Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection", Clin Cancer Res, 15, pp.5473~5477 (2009)
- Alevizos I, "MicroRNA expression profiles as biomarkers of minor salivary gland inflammation and dysfunction in Sjögren's syndrome", Arthritis Rheum, 63, pp.535~544 (2011)
- Xie ZJ, "Saliva supernatant miR-21 : a novel potential biomarker for esophageal cancer detection", Asian Pac J Cancer Prev, 13, pp.6145~6149 (2012)
- Xie YF, "Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues", Int J Oral Sci, 3, pp.125~134 (2011)
- Stoecklin-Wasmer C, "MicroRNAs and their target genes in gingival tissues", J Dent Res, 91, pp.934~940 (2012)
- Ogata Y, "MicroRNA expression in inflamed and noninflamed gingival tissues from Japanese patients", J Oral Sci, 56, pp.253~260 (2014)
- Lee YH, "Comparison of inflammatory microRNA expression in healthy and periodontitis tissues", Biocell, 35, pp.43~49 (2011)
- Perri R, "MicroRNA modulation in obesity and periodontitis", J Dent Res, 91, pp.33~38 (2012)
- Na HS, "Elevated microRNA-128 in periodontitis mitigates tumor necrosis factor-α response via p38 signaling pathway in macrophages", J Periodontol, 87, pp.e173~82 (2016)
- Barad O, "MicroRNA expression detected by oligonucleotide microarrays : system establishment and expression profiling in human tissues", Genome Res, 14, pp.2486~2494 (2004)
- Chen Y, "Reproducibility of quantitative RT-PCR array in miRNA expression profiling and comparison with microarray analysis", BMC Genomics, 10, pp.407~ (2009)
- Mestdagh P, "High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA", Nucleic Acids Res, 36, pp.e143~ (2008)
- Fujimori K, "Detection of salivary miRNAs reflecting chronic periodontitis : a pilot study", Molecules, 24, pp.1034~ (2019)
- Van der Velden U, "What exactly distinguishes aggressive from chronic periodontitis: is it mainly a difference in the degree of bacterial invasiveness?", Periodontol 2000, 75, pp.24~44 (2017)
- Amaral SA, "Comparison of miRNA expression profiles in individuals with chronic or aggressive periodontitis", Oral Dis, 25, pp.561~568 (2019)
- Ghotloo S, "Assessment of microRNA-146a in generalized aggressive periodontitis and its association with disease severity", J Periodontal Res, 54, pp.27~32 (2019)
- Lang N, "Consensus report : aggressive periodontitis", Ann Periodontol, 4, pp.53~ (1999)
- Yoshizawa JM, "Salivary microRNAs and oral cancer detection", Methods Mol Biol, 936, pp.313~324 (2013)
- Li J, "TAM 2.0: tool for MicroRNA set analysis", Nucleic Acids Res, 46, pp.W180~W185 (2018)
- Chou CH, "miRTarBase update 2018 : a resource for experimentally validated microRNA-target interactions", Nucleic Acids Res, 46, pp.D296~D302 (2018)
- Kamburov A, "The ConsensusPathDB interaction database : 2013 update", Nucleic Acids Res, 41, pp.D793~D800 (2013)
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