Anti-nociceptive and anti-inflammatory activities of the essential oil isolated from Cupressus arizonica Greene fruits
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Background: Cupressus arizonica Greene is a coniferous tree with great importance in fragrance and pharmaceutical industries. Essential oils from C. arizonica (EC) have shown potential antioxidant, and anti-microbial activities. This study aimed at investigating the anti-nociceptive and anti-inflammatory effects/mechanisms of EC. Methods: The EC was evaluated for anti-nociceptive and anti-inflammatory activities on male Wistar rats using a formalin test and carrageenan-induced paw edema, respectively. Also, we pre-treated some of the animals with naloxone and flumazenil in the formalin test to find out the possible contributions of opioid and benzodiazepine receptors to EC anti-nociceptive effects. Finally, gas chromatography/mass spectrometry (GC/MS) analysis was used to identify the EC’s constituents. Results: EC in intraperitoneal doses of 0.5 and 1 g/kg significantly decrease the nociceptive responses in both early and late phases of the formalin test. From a mechanistic point of view, flumazenil administration 20 minutes before the most effective dose of EC (1 g/kg) showed a meaningful reduction in the associated antinociceptive responses during the early and late phases of the formalin test. Naloxone also reduced the anti-nociceptive role of EC in the late phase. Furthermore, EC at the doses of 1, 0.5, and 0.25 g/kg significantly reduced paw edema from 0.5 hours after carrageenan injection to 4 hours. GC/MS analysis showed that isolated EC is a monoterpene-rich oil with the major presence of α-pinene (71.92%), myrcene (6.37%), δ-3-carene (4.68%), β-pinene (3.71%), and limonene (3.34%). Conclusions: EC showed potent anti-nociceptive and anti-inflammatory activities with the relative involvement of opioid and benzodiazepine receptors.
Abstract
Background: Cupressus arizonica Greene is a coniferous tree with great importance in fragrance and pharmaceutical industries. Essential oils from C. arizonica (EC) have shown potential antioxidant, and anti-microbial activities. This study aimed at investigating the anti-nociceptive and anti-inflammatory effects/mechanisms of EC. Methods: The EC was evaluated for anti-nociceptive and anti-inflammatory activities on male Wistar rats using a formalin test and carrageenan-induced paw edema, respectively. Also, we pre-treated some of the animals with naloxone and flumazenil in the formalin test to find out the possible contributions of opioid and benzodiazepine receptors to EC anti-nociceptive effects. Finally, gas chromatography/mass spectrometry (GC/MS) analysis was used to identify the EC’s constituents. Results: EC in intraperitoneal doses of 0.5 and 1 g/kg significantly decrease the nociceptive responses in both early and late phases of the formalin test. From a mechanistic point of view, flumazenil administration 20 minutes before the most effective dose of EC (1 g/kg) showed a meaningful reduction in the associated antinociceptive responses during the early and late phases of the formalin test. Naloxone also reduced the anti-nociceptive role of EC in the late phase. Furthermore, EC at the doses of 1, 0.5, and 0.25 g/kg significantly reduced paw edema from 0.5 hours after carrageenan injection to 4 hours. GC/MS analysis showed that isolated EC is a monoterpene-rich oil with the major presence of α-pinene (71.92%), myrcene (6.37%), δ-3-carene (4.68%), β-pinene (3.71%), and limonene (3.34%). Conclusions: EC showed potent anti-nociceptive and anti-inflammatory activities with the relative involvement of opioid and benzodiazepine receptors.
참고문헌 (35)
- Turk DC, "Pain and behavioral medicine: a cognitive-behavioral perspective" (1983), Guilford Press
- Marazziti D, "Pain and psychiatry : a critical analysis and pharmacological review", Clin Pract Epidemiol Ment Health, 2, pp.31~ (2006)
- Fakhri S, "The antinociceptive mechanisms of melatonin : role of L-arginine", nitric oxide, KATP channel signaling pathway, pp.Behav Pharmacol, 2020[DOI]
- Fakhri S, "On the therapeutic targets and pharmacological treatments for pain relief following spinal cord injury : a mechanistic review", Biomed Pharmacother, 139, pp.111563~ (2021)
- Woolf CJ, "What is this thing called pain?", J Clin Invest, 120, pp.3742~3744 (2010)
- Singh BM, "Pain and inflammation : a review", Int J Pharm Sci Res, 3, pp.4697~4709 (2012)
- McCleane G, "Antidepressants as analgesics", CNS Drugs, 22, pp.139~156 (2008)
- Al-Hasani R, "Molecular mechanisms of opioid receptor-dependent signaling and behavior", Anesthesiology, 115, pp.1363~1381 (2011)
- Enna SJ, "The role of GABA in the mediation and perception of pain", Adv Pharmacol, 54, pp.1~27 (2006)
- Arome D, "Pain and inflammation : management by conventional and herbal therapy", Indian J Pain, 28, pp.5~12 (2014)
- Rastegari-Pouyani M, "Antiangiogenesis effect of β-D-mannuronic acid(M2000)as a novel NSAID with immunosuppressive properties under experimental model", Clin Exp Pharmacol Physiol, 45, pp.370~376 (2018)
- Deraedt R, "Release of prostaglandins E and F in an algogenic reaction and its inhibition", Eur J Pharmacol, 61, pp.17~24 (1980)
- Wilhelmsen M, "Analgesic effects of melatonin : a review of current evidence from experimental and clinical studies", J Pineal Res, 51, pp.270~277 (2011)
- Hassanpouraghdam MB, "α-Pinene-and β-myrcene-rich volatile fruit oil of Cupressus arizonica Greene from northwest Iran", Nat Prod Res, 25, pp.634~639 (2011)
- Dubuisson D, "The formalin test : a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats", Pain, 4, pp.161~174 (1977)
- Pandpazir M, "Anti-inflammatory effect and skin toxicity of aqueous extract of Dorema ammoniacum gum in experimental animals", Res J Pharmacogn, 5, pp.1~8 (2018)
- Morris CJ, "Carrageenan-induced paw edema in the rat and mouse", Methods Mol Biol, 225, pp.115~121 (2003)
- quadrupole mass spectroscopy, "Identification of essential oil components by gas chromatography", pp.2001, Adams RP.
- Babushok VI, "Retention indices for frequently reported compounds of plant essential oils", J Phys Chem Ref Data, 40, pp.043101~ (2011)
- Moradi N, "The anti-nociceptive activity of naringenin passes through L-arginine", NO, KATP channel pathway and opioid receptors, pp.Behav Pharmacol, 2021[DOI]
- Taherian AA, "Assessment of aqueous extract of seed of Cuminum cyminum L. on neurogenic and inflammatory pain in mice", J. Med. Plants, 6, pp.44~50 (2007)
- Morteza-Semnani K, "Anti-inf lammatory, analgesic activity and acute toxicity of Glaucium grandiflorum extract", J Ethnopharmacol, 80, pp.181~186 (2002)
- Popović V, "Antinociceptive and antiedematous activities of the essential oils of two Balkan endemic Laserpitium species", Nat Prod Commun, 9, pp.125~128 (2014)
- Emami SA, "Chemical composition and antioxidant activities of the essential oils of different parts of Cupressus arizonica Greene", J Essent Oil Res, 22, pp.193~199 (2010)
- Flamini G, "GC", MS and 13C-NMR integrated analyses of the essential oils from leaves, branches and female cones of Cupressus arizonica from Italy, 2003, pp.15, 302~304
- Biswas SK, "Does the interdependence between oxidative stress and inflammation explain the antioxidant paradox", Oxid Med Cell Longev, 2016, pp.5698931~ (2016)
- Masotti V, "Seasonal and phenological variations of the essential oil from the narrow endemic species Artemisia molinieri and its biological activities", J Agric Food Chem, 51, pp.7115~7121 (2003)
- Chéraif I, "Chemical composition and antimicrobial activity of essential oils of Cupressus arizonica Greene", Biochem Syst Ecol, 35, pp.813~820 (2007)
- Dragomanova S, "Analgesic and anti-inflammatory activity of monoterpenoid Myrtenal in rodents", J IMAB, 25, pp.2406~2413 (2019)
- Kim DS, "Alpha-pinene exhibits anti-inflammatory activity through the suppression of MAPKs and the NF-κB pathway in mouse peritoneal macrophages", Am J Chin Med, 43, pp.731~742 (2015)
- Rufino AT, "Anti-inflammatory and chondroprotective activity of(+)-α-pinene : structural and enantiomeric selectivity", J Nat Prod, 77, pp.264~269 (2014)
- Him A, "Antinociceptive activity of alpha-pinene and fenchone", Pharmacologyonline, 3, pp.363~369 (2008)
- Orhan I, "Bioassayguided evaluation of anti-inflammatory and antinociceptive activities of pistachio, Pistacia vera L", J Ethnopharmacol, 105, pp.235~240 (2006)
- Guimarães AG, "Monoterpenes with analgesic activity--a systematic review", Phytother Res, 27, pp.1~15 (2013)
- Yoon WJ, "Limonene suppresses lipopolysaccharide-induced production of nitric oxide, prostaglandin E2, and pro-inflammatory cytokines in RAW 264.7 macrophages", J Oleo Sci, 59, pp.415~421 (2010)