Assessment of antinociceptive property of Cynara scolymus L. and possible mechanism of action in the formalin and writhing models of nociception in mice
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Cynara scolymus has bioactive constituents and has been used for therapeutic actions. The present study was undertaken to investigate the mechanisms underlying pain-relieving effects of the hydroethanolic extract of C. scolymus (HECS). Methods: The antinociceptive activity of HECS was assessed through formalin and acetic acid-induced writhing tests at doses of 50, 100 and 200 mg/kg intraperitoneally. Additionally, naloxone (non-selective opioid receptors antagonist, 2 mg/kg), atropine (non-selective muscarinic receptors antagonist, 1 mg/kg), chlorpheniramine (histamine H1-receptor antagonist, 20 mg/kg), cimetidine (histamine H2-receptor antagonist, 12.5 mg/kg), flumazenil (GABAA/BDZ receptor antagonist, 5 mg/kg) and cyproheptadine (serotonin receptor antagonist, 4 mg/kg) were used to determine the systems implicated in HECS-induced analgesia. Impact of HECS on locomotor activity was executed by open-field test. Determination of total phenolic content (TPC) and total flavonoid content (TFC) was done. Evaluation of antioxidant activity was conducted employing 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Results: HECS (50, 100 and 200 mg/kg) significantly indicated dose dependent antinociceptive activity against pain-related behavior induced by formalin and acetic acid (P < 0.001). Pretreatment with naloxone, atropine and flumazenil significantly reversed HECS-induced analgesia. Antinociceptive effect of HECS remained unaffected by chlorpheniramine, cimetidine and cyproheptadine. Locomotor activity was not affected by HECS. TPC and TFC of HECS were 59.49 ± 5.57 mgGAE/g dry extract and 93.39 ± 17.16 mgRE/g dry extract, respectively. DPPH free radical scavenging activity (IC50) of HECS was 161.32 ± 0.03 μg/mL. Conclusions: HECS possesses antinociceptive activity which is mediated via opioidergic, cholinergic and GABAergic pathways.
Abstract
Background: Cynara scolymus has bioactive constituents and has been used for therapeutic actions. The present study was undertaken to investigate the mechanisms underlying pain-relieving effects of the hydroethanolic extract of C. scolymus (HECS). Methods: The antinociceptive activity of HECS was assessed through formalin and acetic acid-induced writhing tests at doses of 50, 100 and 200 mg/kg intraperitoneally. Additionally, naloxone (non-selective opioid receptors antagonist, 2 mg/kg), atropine (non-selective muscarinic receptors antagonist, 1 mg/kg), chlorpheniramine (histamine H1-receptor antagonist, 20 mg/kg), cimetidine (histamine H2-receptor antagonist, 12.5 mg/kg), flumazenil (GABAA/BDZ receptor antagonist, 5 mg/kg) and cyproheptadine (serotonin receptor antagonist, 4 mg/kg) were used to determine the systems implicated in HECS-induced analgesia. Impact of HECS on locomotor activity was executed by open-field test. Determination of total phenolic content (TPC) and total flavonoid content (TFC) was done. Evaluation of antioxidant activity was conducted employing 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Results: HECS (50, 100 and 200 mg/kg) significantly indicated dose dependent antinociceptive activity against pain-related behavior induced by formalin and acetic acid (P < 0.001). Pretreatment with naloxone, atropine and flumazenil significantly reversed HECS-induced analgesia. Antinociceptive effect of HECS remained unaffected by chlorpheniramine, cimetidine and cyproheptadine. Locomotor activity was not affected by HECS. TPC and TFC of HECS were 59.49 ± 5.57 mgGAE/g dry extract and 93.39 ± 17.16 mgRE/g dry extract, respectively. DPPH free radical scavenging activity (IC50) of HECS was 161.32 ± 0.03 μg/mL. Conclusions: HECS possesses antinociceptive activity which is mediated via opioidergic, cholinergic and GABAergic pathways.
참고문헌 (48)
- Sreekeesoon DP, "Ethnopharmacological analysis of medicinal plants and animals used in the treatment and management of pain in Mauritius", J Ethnopharmacol, 157, pp.181~200 (2014)
- Olorukooba AB, "Elucidation of the possible mechanism of analgesic action of methanol stem bark extract of Uapaca togoensis pax in mice", J Ethnopharmacol, 245, pp.112156~ (2019)
- Mohammadifard F, "Chemical composition and role of opioidergic system in antinociceptive effect of Ziziphora Clinopodioides essential oil", Basic Clin Neurosci, 9, pp.357~366 (2018)
- Koohsari S, "Antinociceptive and antineuropathic effects of cuminaldehyde, the major constituent of Cuminum cyminum seeds : possible mechanisms of action", J Ethnopharmacol, 255, pp.112786~ (2020)
- Ben Salem M, "Pharmacological studies of artichoke leaf extract and their health benefits", Plant Foods Hum Nutr, 70, pp.441~453 (2015)
- Mejri F, "Phytochemical analysis and in vitro and in vivo evaluation of biological activities of artichoke (Cynara scolymus L.) floral stems: towards the valorization of food by-products", Food Chem, 333, pp.127506~ (2020)
- Salekzamani S, "The antioxidant activity of artichoke(Cynara scolymus) : a systematic review and meta-analysis of animal studies", Phytother Res, 33, pp.55~71 (2019)
- Ben Salem M, "Protective effects of Cynara scolymus leaves extract on metabolic disorders and oxidative stress in alloxan-diabetic rats", BMC Complement Altern Med, 17, pp.328~ (2017)
- Mocelin R, "Hypolipidemic and antiatherogenic effects of Cynara scolymus in cholesterol-fed rats", Rev Bras Farmacognosia, 26, pp.233~239 (2016)
- Ben Salem M, "Chemicals compositions, antioxidant and anti-inflammatory activity of Cynara scolymus leaves extracts, and analysis of major bioactive polyphenols by HPLC", Evid Based Complement Alternat Med, 2017, pp.4951937~ (2017)
- Alahmoradi M, "Protective effect of Cynara scolymus L. on blood biochemical parameters and liver histopathological changes in phenylhydrazine-induced hemolytic anemia in rats", Pharm Biomed Res, 5, pp.53~62 (2019)
- Allahmoradi M, "Amelioration of lipid peroxidation and antioxidant enzymes status in the serum and erythrocytes of phenylhydrazine-induced anemic male rats: the protective role of artichoke extract (Cynara scolymus L.)", Iran J Vet Med, 14, pp.315~328 (2020)
- Zimmermann M, "Ethical guidelines for investigations of experimental pain in conscious animals", Pain, 16, pp.109~110 (1983)
- Kavaliers M, "Daily rhythms of analgesia in mice : effects of age and photoperiod", Brain Res, 279, pp.387~393 (1983)
- Montiel-Ruiz RM, "Synergistic interactions between the antinociceptive effect of Rhodiola rosea extract and B vitamins in the mouse formalin test", Phytomedicine, 20, pp.1280~1287 (2013)
- Zendehdel M, "Antinociceptive mechanisms of Bunium persicum essential oil in the mouse writhing test : role of opioidergic and histaminergic systems", Vet Med, 60, pp.63~70 (2015)
- Oliveira AS, "The ethanol extract of Leonurus sibiricus L. induces antioxidant, antinociceptive and topical anti-inflammatory effects", J Ethnopharmacol, 206, pp.144~151 (2017)
- Zhishen J, "The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals", Food Chem, 64, pp.555~559 (1999)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Khalil M, "Antisteatotic and antioxidant activities of Thymbra spicata L. extracts in hepatic and endothelial cells as in vitro models of non-alcoholic fatty liver disease", J Ethnopharmacol, 239, pp.111919~ (2019)
- Tamaddonfard E, "Effect of crocin on the morphine-induced antinociception in the formalin test in rats", Phytother Res, 24, pp.410~413 (2010)
- Dubuisson D, "The formalin test : a quantitative study of the analgesic effects of morphine, meperidine, and brain stem stimulation in rats and cats", Pain, 4, pp.161~174 (1977)
- Abbott FV, "Options for management of acute pain in the rat", Vet Rec, 140, pp.553~557 (1997)
- Koster R, "Acetic acid for analgesic screening", Fed Proc, 18, pp.412~417 (1959)
- Hishe HZ, "Antinociceptive effect of methanol extract of leaves of Senna singueana in mice", J Ethnopharmacol, 217, pp.49~53 (2018)
- Yam MF, "General pathways of pain sensation and the major neurotransmitters involved in pain regulation", Int J Mol Sci, 19, pp.2164~ (2018)
- Hunskaar S, "The formalin test in mice : dissociation between inflammatory and non-inflammatory pain", Pain, 30, pp.103~114 (1987)
- Ribeiro RA, "Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice", Eur J Pharmacol, 387, pp.111~118 (2000)
- Karbab A, "Anti-inflammatory, analgesic activity, and toxicity of Pituranthos scoparius stem extract : an ethnopharmacological study in rat and mouse models", J Ethnopharmacol, 258, pp.112936~ (2020)
- Ferraz CR, "Therapeutic potential of flavonoids in pain and inflammation:mechanisms of action, pre-clinical and clinical data, and pharmaceutical development", Molecules, 25, pp.762~ (2020)
- Bagdas D, "Antinociceptive effect of chlorogenic acid in rats with painful diabetic neuropathy", J Med Food, 17, pp.730~732 (2014)
- dos Santos MD, "Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid", Biol Pharm Bull, 29, pp.2236~2240 (2006)
- Shan J, "Chlorogenic acid inhibits lipopolysaccharideinduced cyclooxygenase-2 expression in RAW264.7cells through suppressing NF-kappaB and JNK", AP-1activation, 2009, pp.9, 1042~1048
- Pinheiro MM, "Antinociceptive effect of the Orbignya speciosa Mart. (Babassu)leaves: evidence for the involvement of apigenin", Life Sci, 91, pp.293~300 (2012)
- Aziz N, "Anti-inflammatory effects of luteolin : a review of in vitro, in vivo, and in silico studies", J Ethnopharmacol, 225, pp.342~358 (2018)
- Miño J, "Antinociceptive effect of the aqueous extract of Balbisia calycina", J Ethnopharmacol, 79, pp.179~182 (2002)
- Filho AW, "Quercetin : further investigation of its antinociceptive properties and mechanisms of action", Arch Pharm Res, 31, pp.713~721 (2008)
- Toker G, "Flavonoids with antinociceptive and anti-inflammatory activities from the leaves of Tilia argentea(silver linden)", J Ethnopharmacol, 95, pp.393~397 (2004)
- Yousofvand N, "An overview of the effect of medicinal herbs on pain", Phytother Res, 37, pp.1057~1081 (2023)
- Bodnar RJ, "Endogenous opiates and behavior : 2018", Peptides, 132, pp.170348~ (2020)
- Eisenach JC, "Muscarinic-mediated analgesia", Life Sci, 64, pp.549~554 (1999)
- Naser PV, "Molecular, cellular and circuit basis of cholinergic modulation of pain", Neuroscience, 387, pp.135~148 (2018)
- Goudet C, "Metabotropic receptors for glutamate and GABA in pain", Brain Res Rev, 60, pp.43~56 (2009)
- Mahdian Dehkordi F, "The antinociceptive effect of artemisinin on the inflammatory pain and role of GABAergic and opioidergic systems", Korean J Pain, 32, pp.160~167 (2019)
- Hara K, "Chlorogenic acid administered intrathecally alleviates mechanical and cold hyperalgesia in a rat neuropathic pain model", Eur J Pharmacol, 723, pp.459~464 (2014)
- Obara I, "Histamine, histamine receptors, and neuropathic pain relief", Br J Pharmacol, 177, pp.580~599 (2020)
- Li P, "Cholinergic, noradrenergic, and serotonergic inhibition of fast synaptic transmission in spinal lumbar dorsal horn of rat", Brain Res Bull, 54, pp.639~647 (2001)
- Martinello K, "5-HT3 receptors in rat dorsal root ganglion neurons : Ca2+ entry and modulation of neurotransmitter release", Life (Basel), 12, pp.1178~ (2022)
