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Sinus augmentation with poly(ε) caprolactone-β tricalcium phosphate scaffolds, mesenchymal stem cells and platelet rich plasma for one-stage dental implantation in minipigs

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Purpose: This study evaluated the efficacy of a tube-shaped poly(ε) caprolactone - β tricalcium phosphate (PCL-TCP) scaffold with the incorporation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) and platelet-rich plasma (PRP) for bone regeneration in the procedure of single-stage sinus augmentation and dental implantation in minipigs. Methods: Implants were placed in the bilateral sides of the maxillary sinuses of 5 minipigs and allocated to a PCL-TCP+hUCMSCs+PRP group (n=5), a PCL-TCP+PRP group (n=5), and a PCL-TCP-only group (n=6). After 12 weeks, bone regeneration was evaluated with soft X-rays, micro-computed tomography, fluorescence microscopy, and histomorphometric analysis. Results: Four implants failed (2 each in the PCL-TCP+hUCMSCs+PRP and PCLTCP+hUCMSC groups). An analysis of the grayscale levels and bone-implant contact ratio showed significantly higher mean values in the PCL-TCP+hUCMSCs+PRP than in the PCL-TCP group (P=0.045 and P=0.016, respectively). In fluoromicroscopic images, new bone formation around the outer surfaces of the scaffolds was observed in the PCLTCP+hUCMSCs+PRP group, suggesting a tenting effect of the specially designed scaffolds. Bone regeneration at the scaffold-implant interfaces was observed in all 3 groups. Conclusions: Using a tube-shaped, honeycombed PCL-TCP scaffold with hUCMSCs and PRP may serve to enhance bone formation and dental implants’ osseointegration in the procedure of simultaneous sinus lifting and dental implantation.

Abstract

Purpose: This study evaluated the efficacy of a tube-shaped poly(ε) caprolactone - β tricalcium phosphate (PCL-TCP) scaffold with the incorporation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) and platelet-rich plasma (PRP) for bone regeneration in the procedure of single-stage sinus augmentation and dental implantation in minipigs. Methods: Implants were placed in the bilateral sides of the maxillary sinuses of 5 minipigs and allocated to a PCL-TCP+hUCMSCs+PRP group (n=5), a PCL-TCP+PRP group (n=5), and a PCL-TCP-only group (n=6). After 12 weeks, bone regeneration was evaluated with soft X-rays, micro-computed tomography, fluorescence microscopy, and histomorphometric analysis. Results: Four implants failed (2 each in the PCL-TCP+hUCMSCs+PRP and PCLTCP+hUCMSC groups). An analysis of the grayscale levels and bone-implant contact ratio showed significantly higher mean values in the PCL-TCP+hUCMSCs+PRP than in the PCL-TCP group (P=0.045 and P=0.016, respectively). In fluoromicroscopic images, new bone formation around the outer surfaces of the scaffolds was observed in the PCLTCP+hUCMSCs+PRP group, suggesting a tenting effect of the specially designed scaffolds. Bone regeneration at the scaffold-implant interfaces was observed in all 3 groups. Conclusions: Using a tube-shaped, honeycombed PCL-TCP scaffold with hUCMSCs and PRP may serve to enhance bone formation and dental implants’ osseointegration in the procedure of simultaneous sinus lifting and dental implantation.

참고문헌 (37)

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관련 논문AI추천

Bone regeneration potential of poly(ε-caprolactone)/β-tricalcium phosphate polymer scaffolds compared with collagen-reinforced synthetic and xenogenic scaffolds in rabbit calvarial defectsByung-Hoon Kim, Jae-Ha Baek, Keon-Il Yang, Sang-Joun Yu, Seok-Hwan Jeong, Seol Kim, Seung-Hwan Seol치의학연구원 · Oral Biology Research2025 · 제49권 제3호Effects of Polycaprolactone-Tricalcium Phosphate, Recombinant Human Bone Morphogenetic Protein-2 and Dog Mesenchymal Stem Cells on Bone Formation: Pilot Study in Dogs김명래, 김선종, 신상완, 오진섭, 한인호연세대학교의과대학 · Yonsei Medical Journal2009 · 제50권 제6호Late-term healing in an augmented sinus with different ratios of biphasic calcium phosphate: a pilot study using a rabbit sinus model이중석, 임현창, 정의원, 최성호, 홍지연대한치주과학회 · Journal of Periodontal & Implant Science2016 · 제46권 제1호Rabbit maxillary sinus augmentation model with simultaneous implant placement: differential responses to the graft materials김경화, 김수환, 김영성, 설양조, 이영규, 이용무, 진민주대한치주과학회 · Journal of Periodontal & Implant Science2012 · 제42권 제6호Sinus augmentation using rhBMP-2-loaded synthetic bone substitute with simultaneous implant placement in rabbits임현창, 정의원, 주명재, 차재국, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2017 · 제47권 제2호Bone formation around rhBMP-2- coated implants in rabbit sinuses with or without absorbable collagen sponge grafting백원선, 윤소라, 이중석, 임현창, 정의원, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2015 · 제45권 제6호

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