흰쥐 초산 유도 십이지장 궤양 치유과정에서 TGF-beta 및 그 수용체의 발현

Abstract

Background : Many works have been written about the transforming growth factor-beta 1 (TGF-β1) which is closely associated with fibrosis in the inflammatory conditions of different organs. TGF-β1 exerts its biological effects by interacting with specific cell surface receptors, namely, transforming growth factor-beta receptor type I and II (TGF βRI and TGF βRII). Methods : To investigate the temporal expressions and localizations of TGF-β1, TGF βRI, and TGF βRII in acetic acid-induced duodenal ulcerated tissues, we performed in situ hybridization and immunohistochemical techniques. Results : Under in situ hybridization, TGF-β1, TGF βRI, and TGF βRII mRNA signals increased in the experimental groups (1, 3, 7, and 14 day groups) compared to those of the control group. The signals on day 14 decreased slightly compared to those of days 1, 3, and 7, but they were higher than those of the control group. Under immunohistochemical study, TGF-β1, TGF βRI, and TGF βRII were localized in the mucosal epithelial cells and in the macrophages, vascular endothelial cells, and fibroblasts of the lamina propria and granulation tissue. As in the case of the in situ hybridization, it revealed that the expression of three proteins increased in the experimental groups compared to that of the control group. The expression on day 14 decreased compared to those of days 1, 3, and 7, but it was more intense than that of the control group. Conclusions : This study suggests that TGF-β1, TGF βRI, and TGF βRII contribute to the early stage healing of duodenal ulcer.