Expression of Claudin-1, p53 and E-cadherin in Pseudoepitheliomatous Hyperplasia and Squamous Cell Carcinoma of the Head and Neck
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Background : Pseudoepitheliomatous hyperplasia (PEH) is a reactive proliferation of surface epithelium and can be confused with invasive squamous cell carcinoma (SCC) in head and neck biopsy specimens. To distinguish PEH from invasive SCC, immunohistochemical staining for claudin-1, E-cadherin and p53 was performed. Methods : Eighteen cases of PEH and 29 invasive SCC from head and neck lesions were immunostained and examined. Results : The invasive SCC showed increased staining of claudin-1 (p<0.001) and p53 (p<0.001) and decreased staining of E-cadherin (p=0.005) compared to the PEH specimens. The combined score calculated by adding the positive sum of claudin-1 and p53 and subtracting E-cadherin was useful for the differentiation of SCC from PEH (89.7% sensitivity and 88.9% specificity, p<0.001). Conclusions : The combined immunostaining for claudin-1, p53 and E-cadherin may help differentiate PEH from invasive SCC. The results of this study suggest that the increased expression of claudin-1 and p53 and the decreased expression of E-cadherin maybe markers for the aggressive growth of invasive SCC.
Abstract
Background : Pseudoepitheliomatous hyperplasia (PEH) is a reactive proliferation of surface epithelium and can be confused with invasive squamous cell carcinoma (SCC) in head and neck biopsy specimens. To distinguish PEH from invasive SCC, immunohistochemical staining for claudin-1, E-cadherin and p53 was performed. Methods : Eighteen cases of PEH and 29 invasive SCC from head and neck lesions were immunostained and examined. Results : The invasive SCC showed increased staining of claudin-1 (p<0.001) and p53 (p<0.001) and decreased staining of E-cadherin (p=0.005) compared to the PEH specimens. The combined score calculated by adding the positive sum of claudin-1 and p53 and subtracting E-cadherin was useful for the differentiation of SCC from PEH (89.7% sensitivity and 88.9% specificity, p<0.001). Conclusions : The combined immunostaining for claudin-1, p53 and E-cadherin may help differentiate PEH from invasive SCC. The results of this study suggest that the increased expression of claudin-1 and p53 and the decreased expression of E-cadherin maybe markers for the aggressive growth of invasive SCC.
참고문헌 (28)
- Sagi A, "Atypical pseudoepitheliomatous hyperplasia in squamous cell carcinoma in chronic wounds", Plast Reconstr Surg, 74, pp.315~316 (1984)
- Lee YS, "p53 expression in pseudoepitheliomatous hyperplasia, keratoacanthoma, and squamous cell carcinoma of skin", Cancer, 73, pp.2317~2323 (1994)
- Zarovnaya E, "Distinguishing pseudoepitheliomatous hyperplasia from squamous cell carcinoma in mucosal biopsy specimens from the head and neck", Arch Pathol Lab Med, 129, pp.1032~1036 (2005)
- 오훈규, "거짓상피종증식을 동반한 코형 NK", T세포림프종 - 1예 보고 -, 2006, pp.40(3), 250~254[DOI]
- Al Kassam D, "Diagnostic value of E-cadherin, MMP-9, activated MMP-13 and anti-p53 antibodies in squamous cell carcinomas of head and neck", Med Clin (Barc), 129, pp.761~765 (2007)
- Impola U, "Expression of matrix metalloproteinase (MMP)-7 and MMP-13 and loss of MMP-19 and p16 are associated with malignant progression in chronic wounds", Br J Dermatol, 152, pp.720~726 (2005)
- 정 윤, "만성피부궤양과 편평세포암의 MMP-7과 MMP-13 발현에 관한 면역조직화학적 연구", 대한피부과학회지, 44(11), pp.1284~1289 (2006), 대한피부과학회
- Gonzalez-Mariscal L, "Tight junction proteins", Prog Biophys Mol Biol, 81, pp.1~44 (2003)
- Morita K, "Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands", Proc Natl Acad Sci U S A, 96, pp.511~516 (1999)
- Tebbe B, "Tight junction proteins: a novel class of integral membrane proteins. Expression in human epidermis and in HaCaT keratinocytes", Arch Dermatol Res, 294, pp.14~18 (2002)
- Sobel G, "Increased expression of claudins in cervical squamous intraepithelial neoplasia and invasive carcinoma", Hum Pathol, 36, pp.162~169 (2005)
- Kinugasa T, "Selective up-regulation of claudin-1 and claudin-2 in colorectal cancer", Anticancer Res, 27, pp.3729~3734 (2007)
- Scott G, "Pseudoepitheliomatous hyperplasia in Spitz nevi. A possible source of confusion with squamous cell carcinoma", Arch Pathol Lab Med, 113, pp.61~63 (1989)
- Biswas A, "Recurrent primary cutaneous lymphoma with florid pseudoepitheliomatous hyperplasia masquerading as squamous cell carcinoma", Histopathology, 52, pp.755~758 (2008)
- Calhoun KH, "Pseudoepitheliomatous hyperplasia mistaken for cancer after delayed reconstruction", South Med J, 88, pp.454~457 (1995)
- Gacek MR, "Pseudoepitheliomatous hyperplasia versus squamous cell carcinoma of the external auditory canal", Laryngoscope, 108, pp.620~623 (1998)
- Galan A, "Langerhans cells in squamous cell carcinoma vs. pseudoepitheliomatous hyperplasia of the skin", J Cutan Pathol, 34, pp.950~952 (2007)
- Katoh M, "CLDN23 gene, frequently down-regulated in intestinal-type gastric cancer, is a novel member of CLAUDIN gene family", Int J Mol Med, 11, pp.683~689 (2003)
- Tsukita S, "Multifunctional strands in tight junctions", Nat Rev Mol Cell Biol, 2, pp.285~293 (2001)
- Ivanov AI, "Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment", Mol Biol Cell, 15, pp.176~188 (2004)
- Paschoud S, "Claudin-1 and claudin- 5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas", Mod Pathol, 20, pp.947~954 (2007)
- Cohn ML, "Loss of claudin-1 expression in tumor-associated vessels correlates with acquisition of metastatic phenotype in melanocytic neoplasms", J Cutan Pathol, 32, pp.533~536 (2005)
- Tokes AM, "Claudin-1, -3 and -4 proteins and mRNA expression in benign and malignant breast lesions: a research study", Breast Cancer Res, 7, pp.296~305 (2005)
- Kurtz KA, "Decreased E-cadherin but not beta-catenin expression is associated with vascular invasion and decreased survival in head and neck squamous carcinomas", Otolaryngol Head Neck Surg, 134, pp.142~146 (2006)
- Greenblatt MS, "Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis", Cancer Res, 54, pp.4855~4878 (1994)
- Batinac T, "p53 protein expression and cell proliferation in non-neoplastic and neoplastic proliferative skin diseases", Tumori, 90, pp.120~127 (2004)
- Oliveira LR, "Prognostic significance of p53 and p63 immunolocalisation in primary and matched lymph node metastasis in oral squamous cell carcinoma", Acta Histochem, 109, pp.388~396 (2007)
- Shin DM, "p53 expressions: predicting recurrence and second primary tumors in head and neck squamous cell carcinoma", J Natl Cancer Inst, 88, pp.519~529 (1996)