전이성 대장암종에서 Osteopontin 및 MMP-1의 유전자 발현 변화 및 연관 임상 소견 검증
Validation of Gene Expression Changes of Osteopontin and MMP-1 in Primary and Metastatic Colorectal Carcinomas
2
조회
0
다운로드
0
인용
0
참고문헌
초록
Background : Metastasis is one of the most important characteristics of cancer in terms of its impact on patient survival. Unfortunately, identification of altered genes during tumor metastasis is limited. Methods : Using high-throughput microarrays containing 19K spotted human oligonucleotides, gene expression of primary and matched metastatic colon cancer were compared in previous study. Although DNA microarray analysis did not demonstrate complete classification of primary and metastatic carcinoma, 80 differentially expressed genes were identified. Among these, expression of osteopontin, matrix metalloproteinase-1 (MMP-1) and serpin A1 was assessed using immunohistochemistry in a validation set containing 43 pairs from tissue microarrays. Results : The expression of osteopontin was significantly higher in metastatic carcinoma than in primary carcinoma, as indicated by mRNA expression. The expression of MMP-1 was significantly lower in metastatic carcinoma. Expression of serpin A1 was not correlated with the microarray results. Conclusions : Osteopontin and MMP-1 expression successfully classified primary and metastatic colorectal carcinomas and further studies on their clinical application is encouraged.
Abstract
Background : Metastasis is one of the most important characteristics of cancer in terms of its impact on patient survival. Unfortunately, identification of altered genes during tumor metastasis is limited. Methods : Using high-throughput microarrays containing 19K spotted human oligonucleotides, gene expression of primary and matched metastatic colon cancer were compared in previous study. Although DNA microarray analysis did not demonstrate complete classification of primary and metastatic carcinoma, 80 differentially expressed genes were identified. Among these, expression of osteopontin, matrix metalloproteinase-1 (MMP-1) and serpin A1 was assessed using immunohistochemistry in a validation set containing 43 pairs from tissue microarrays. Results : The expression of osteopontin was significantly higher in metastatic carcinoma than in primary carcinoma, as indicated by mRNA expression. The expression of MMP-1 was significantly lower in metastatic carcinoma. Expression of serpin A1 was not correlated with the microarray results. Conclusions : Osteopontin and MMP-1 expression successfully classified primary and metastatic colorectal carcinomas and further studies on their clinical application is encouraged.
참고문헌 (28)
- Kumar V, "Robbins and Cotran pathologic basis of disease. 7th ed" (2005), Elsevier Saunders
- Sunami E, "MMP-1 is a prognostic marker for hematogenous metastasis of colorectal cancer", Oncologist, 5, pp.108~114 (2000)
- Ramaswamy S, "A molecular signature of metastasis in primary solid tumors", Nat Genet, 33, pp.49~54 (2003)
- van’t Veer LJ, "Gene expression profiling predicts clinical outcome of breast cancer", Nature, 415, pp.530~536 (2002)
- Bertucci F, "Gene expression profiling and clinical outcome in breast cancer", OMICS, 10, pp.429~443 (2006)
- Bertucci F, "Gene expression profiling of colon cancer by DNA microarrays and correlation with histoclinical parameters", Oncogene, 23, pp.1377~1391 (2004)
- Koh KH, "Differential gene expression profiles of metastases in paired primary and metastatic colorectal carcinomas", Oncology, 75, pp.92~101 (2008)
- Ward DG, "Identification of serum biomarkers for colon cancer by proteomic analysis", Br J Cancer, 94, pp.1898~905 (2006)
- Wai PY, "Osteopontin:regulation in tumor metastasis", Cancer Metastasis Rev, 27, pp.103~118 (2008)
- Senger DR, "Adhesive properties of osteopontin:regulation by a naturally occurring thrombin-cleavage in close proximity to the GRGDS cell-binding domain", Mol Biol Cell, 5, pp.565~574 (1994)
- Tuck AB, "Osteopontin induces increased invasiveness and plasminogen activator expression of human mammary epithelial cells", Oncogene, 18, pp.4237~4246 (1999)
- Ue T, "Co-expression of osteopontin and CD44v9 in gastric cancer", Int J Cancer, 79, pp.127~132 (1998)
- Chambers AF, "Osteopontin expression in lung cancer", Lung Cancer, 15, pp.311~323 (1996)
- Thalmann GN, "Osteopontin:possible role in prostate cancer progression", Clin Cancer Res, 5, pp.2271~2277 (1999)
- Pan HW, "Overexpression of osteopontin is associated with intrahepatic metastasis,early recurrence,and poorer prognosis of surgically resected hepatocellular carcinoma", Cancer, 98, pp.119~127 (2003)
- Agrawal D, "Osteopontin identified as lead marker of colon cancer progression,using pooled sample expression profiling", J Natl Cancer Inst, 94, pp.513~521 (2002)
- Yoneda T, "Promotion of angiogenesis and enhancement of breast cancer metastasis to bone", Bone, 23, pp.201~ (1998)
- Oates AJ, "The identification of osteopontin as a metastasis-related gene product in a rodent mammary tumour model", Oncogene, 13, pp.97~104 (1996)
- Wu Y, "Osteopontin is required for full expression of the transformed phenotype by the ras oncogene", Br J Cancer, 83, pp.156~163 (2000)
- El-Tanani MK, "Regulatory region of metastasis-inducing DNA is the binding site for T cell factor-4", Oncogene, 20, pp.1793~1797 (2001)
- Rohde F, "Expression of osteopontin,a target gene of de-regulated Wnt signaling,predicts survival in colon cancer", Int J Cancer, 121, pp.1717~1723 (2007)
- Deryugina EI, "Matrix metalloproteinases and tumor metastasis", Cancer Metastasis Rev, 25, pp.9~34 (2006)
- Nikkola J, "High serum levels of matrix metalloproteinase-9 and matrix metalloproteinase-1 are associated with rapid progression in patients with metastatic melanoma", Clin Cancer Res, 11, pp.5158~5166 (2005)
- Murray GI, "Matrix metalloproteinase-1 is associated with poor prognosis in colorectal cancer", Nat Med, 2, pp.461~462 (1996)
- Crystal RG, "The alpha 1-antitrypsin gene and its deficiency states", Trends Genet, 5, pp.411~417 (1989)
- de Sá SV, "Serpin peptidase inhibitor clade A member 1 as a potential marker for malignancy in insulinomas", Clin Cancer Res, 13, pp.5322~5330 (2007)
- Duplantier MM, "Serpin A1 is overexpressed in ALK+ anaplastic large cell lymphoma and its expression correlates with extranodal dissemination", Leukemia, 20, pp.1848~1854 (2006)
- Patz EF Jr, "Panel of serum biomarkers for the diagnosis of lung cancer", J Clin Oncol, 25, pp.5578~5583 (2007)