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육종에서 p53, c-kit과 CD34의 발현에 대한 조직 미세배열을 이용한 연구

Tissue Microarray Analysis of the Expression of p53, c-kit and CD34 in Sarcomas

박경신|강석진|강창석|유진영
대한병리학회|2004.08|Journal of Pathology and Translational Medicine|제38권 제4호|pp. 221-227

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참고문헌

주제분류병리학

초록

Abstract

Background : Our objectives in this study were to (1) evaluate the possible role of p53, c-kit and CD34 proteins in sarcomas and to determine their potential relationship; (2) use a tissue microarray to compare the immunohistochemical staining results on both the tissue microarrays and the corresponding whole tissue sections. Methods : Whole sections from 85 sarcomas were studied for the immunohistochemical expression of p53, c-kit and CD34. Tissue microarrays consisting of triplicate 2 mm cores from the corresponding blocks were constructed and stained according to the same protocols as those used for the whole sections. Results : On whole section analysis, p53 protein was expressed in 25 cases (29.4%). Expression of c-kit was observed in 31 specimens (36.5%), whereas CD34 expression was noted in 11 tumors (12.9%). The overall concordance between triplicates was 96% (217/226). The consensus score from the combined triplicates agreed with the results on the whole sections at 91.4% (233/255). The correlations between p53 and CD34, and between c-kit and CD34, were statistically significant (p=.028 and p=.010 respectively). Conclusions : p53 and c-kit express relatively frequently in sarcomas. Tissue microarrays are an effective alternative to whole sections; however, the presence of triplicate punches seems to improve the yield but not the concordance of data.

참고문헌 (29)

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