Loss of aquaporin-1 expression is associated with worse clinical outcomes in clear cell renal cell carcinoma: an immunohistochemical study
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Aquaporin (AQP) expression has been investigated in various malignant neoplasms, and the overexpression of AQP is related to poor prognosis in some malignancies. However, the expression of AQP protein in clear cell renal cell carcinoma (ccRCC) has not been extensively investigated by immunohistochemistry with large sample size. Methods: We evaluated the AQP expression in 827 ccRCC with immunohistochemical staining in tissue microarray blocks and classified the cases into two categories, high and low expression. Results: High expression of aquaporin-1 (AQP1) was found in 320 cases (38.7%), but aquaporin-3 was not expressed in ccRCC. High AQP1 expression was significantly related to younger age, low TNM stage, low World Health Organization/International Society of Urologic Pathology nuclear grade, and absence of distant metastasis. Furthermore, high AQP1 expression was also significantly associated with longer overall survival (OS; p<.001) and progression-specific survival (PFS; p<.001) and was an independent predictor of OS and PFS in ccRCC. Conclusions: Our study revealed the prognostic significance of AQP1 protein expression in ccRCC. These findings could be applied to predict the prognosis of ccRCC.
Abstract
Background: Aquaporin (AQP) expression has been investigated in various malignant neoplasms, and the overexpression of AQP is related to poor prognosis in some malignancies. However, the expression of AQP protein in clear cell renal cell carcinoma (ccRCC) has not been extensively investigated by immunohistochemistry with large sample size. Methods: We evaluated the AQP expression in 827 ccRCC with immunohistochemical staining in tissue microarray blocks and classified the cases into two categories, high and low expression. Results: High expression of aquaporin-1 (AQP1) was found in 320 cases (38.7%), but aquaporin-3 was not expressed in ccRCC. High AQP1 expression was significantly related to younger age, low TNM stage, low World Health Organization/International Society of Urologic Pathology nuclear grade, and absence of distant metastasis. Furthermore, high AQP1 expression was also significantly associated with longer overall survival (OS; p<.001) and progression-specific survival (PFS; p<.001) and was an independent predictor of OS and PFS in ccRCC. Conclusions: Our study revealed the prognostic significance of AQP1 protein expression in ccRCC. These findings could be applied to predict the prognosis of ccRCC.
참고문헌 (16)
- Agre P, "Aquaporin water channels : from atomic structure to clinical medicine", J Physiol, 542, pp.3~16 (2002)
- Chen J, "Aquaporin 3 promotes prostate cancer cell motility and invasion via extracellular signal-regulated kinase 1", 2-mediated matrix metalloproteinase-3 secretion, 2015, pp.11, 2882~2888
- Kusayama M, "Critical role of aquaporin 3on growth of human esophageal and oral squamous cell carcinoma", Cancer Sci, 102, pp.1128~1136 (2011)
- Yang J, "Effects of AQP5 gene silencing on proliferation, migration and apoptosis of human glioma cells through regulating EGFR", ERK, Oncotarget, pp.2017, 8[DOI]
- Kang BW, "Expression of aquaporin-1, aquaporin-3, and aquaporin-5 correlates with nodal metastasis in colon cancer", Oncology, 88, pp.369~376 (2015)
- Moosavi MS, "Aquaporins 1, 3 and 5 in different tumors, their expression, prognosis value and role as new therapeutic targets", Pathol Oncol Res, 26, pp.615~625 (2020)
- Ticozzi-Valerio D, "Differential expression of AQP1 in microdomain-enriched membranes of renal cell carcinoma", Proteomics Clin Appl, 1, pp.588~597 (2007)
- Huang Y, "Expression of aquaporin 1 in primary renal tumors : a prognostic indicator for clear-cell renal cell carcinoma", Eur Urol, 56, pp.690~698 (2009)
- Otto W, "Loss of aquaporin 3 protein expression constitutes an independent prognostic factor for progression-free survival : an immunohistochemical study on stage pT1 urothelial bladder cancer", BMC Cancer, 12, pp.459~ (2012)
- Bedford JJ, "Aquaporin expression in normal human kidney and in renal disease", J Am Soc Nephrol, 14, pp.2581~2587 (2003)
- Sato K, "Different prognostic implications of aquaporin-1 and aquaporin-5 expression among different histological types of ovarian carcinoma", Pathol Oncol Res, 26, pp.263~271 (2020)
- Zou W, "AQP1 and AQP3 expression are associated with severe symptoms and poor-prognosis of the pancreatic ductal adenocarcinoma", Appl Immunohistochem Mol Morphol, 27, pp.40~47 (2019)
- Li C, "Elevated AQP1 expression is associated with unfavorable oncologic outcome in patients with hilar cholangiocarcinoma", Technol Cancer Res Treat, 16, pp.421~427 (2017)
- Xu WH, "Prognostic implications of aquaporin 9expression in clear cell renal cell carcinoma", J Transl Med, 17, pp.363~ (2019)
- Dorward HS, "Pharmacological blockade of aquaporin-1 water channel by AqB013 restricts migration and invasiveness of colon cancer cells and prevents endothelial tube formation in vitro", J Exp Clin Cancer Res, 35, pp.36~ (2016)
- Jiang B, "miR-874 Inhibits cell proliferation, migration and invasion through targeting aquaporin-3 in gastric cancer", J Gastroenterol, 49, pp.1011~1025 (2014)