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백서 척수후근절의 슈반세포 배양을 위한 Ara-C 분열억제제의 최적 효과에 대한 연구

The most appropriate antimitotic treatment of Ara-C in Schwann cell-enriched culture from dorsal root ganglia of new born rat

이종호|장정원|김성민
대한구강악안면외과학회 (Korean Association Of Oral And Maxillofacial Surgeons)|2006.02|대한구강악안면외과학회지 (Journal of the Korean Association of Oral and Maxillofacial Surgeons)|제32권 제1호|pp. 42-51

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참고문헌

주제분류치의학

초록

Abstract

Schwann cell, one of important components of peripheral nervous system, interact with neurons to mutually support the growth and replication of embryonal nerves and to maintain the different functions of adult nerves. The Ara-C, known as an antimitotic agent, have been used to have high effectiveness in eliminating fibroblasts during Schwann cell culture period. This enrichment effect is also known to be cummulative with each successive pulse of Ara-C applied and is due to a progressive loss of fibroblasts. But the cytotoxicity by Ara-C is also cummulative and noticeable over the period. To determine the most effective application time and interval of Ara-C in the Schwann cell culture, we observed the Schwann cell purity and density with the Ara-C treatment in plain and three-dimensional culture from dorsal root ganglion of new born rat. By culturing dispersed dorsal root ganglia, we can repeatedly generate homogenous Schwann cells, and cellular morphology and cell count with mean percentages were evaluated in the plain culture dishes and in the immunostainings of S-100 and GFAP in the three-dimensional culture. The Ara-C treated cultures showed a higher Schwann cell percentage (31.0%±8.09% in P4 group to 65.5%±24.08% in P2 group), compared with that obtained in the abscence of Ara-C (17.6%±6.03%) in the plain culture after 2 weeks. And in the three-dimensional culture, S-100 positive cells increased to 56.22%±0.67% and GFAP positive cells to 66.46%±1.83% in G2 group (p<0.05), higher yield than other groups with Ara-C application. Therefore, we concluded that the Ara-C treatment is effective for the proliferation of Schwann cells contrast to the fibroblasts in vitro culture, and the first application after 24 hours from cell harvesting and subsequent 2 pulse treatment (P2 group in plain culture and G2 group in three-dimensional culture) was more effective than other application protocols.

참고문헌 (25)

  1. "Schwann cells as regula-tors of nerve development", 96, pp.17~24 (2002)
  2. "Making of a Schwann", 12, pp.84~86 (1996)
  3. "Effect of Schwanncells in the enhancement of peripheral-nerve regeneration", 12, pp.439~446 (1996)
  4. "The influence of heregulins onhuman Schwann cell proliferation", 15, pp.1329~1340 (1995)
  5. "Studies on culture ratSchwann cells.I.Establishment of purified populationsfrom cultures of peripheral nerve", 165, pp.105~118 (1979)
  6. "Purification and expansion of human Schwann cells invitro", 1, pp.80~83 (1995)
  7. "Selective expansion and long-term culture ofhuman Schwann cell from sural nerve biopsies", 38, pp.674~678 (1995)
  8. "Axolemma is amitogen for human Schwann cells", 15, pp.449~452
  9. "Schwann cell-enriched cultures from adult human periph-eral nerve a technique combining short enzymatic dissoci-The most appropriate antimitotic treatment of Ara-C in Schwann cell-enriched culture from dorsal root ganglia of new born rat 51ation and treatment with cytosine arabinoside", 114, pp.1~8 (2002)
  10. "Isolation of activatedadult Schwann cells and a spontaneously immortalSchwann cell clone", 33, pp.231~238 (1992)
  11. "Improved protocol forschwann cell isolation and proliferation from rat dorsalroot ganglia in vitro", 25, pp.265~272 (2003)
  12. "Culture of animal cells a manual of basictechnique", pp.89~104 (2000), Wiley-Liss Inc
  13. "Fabrication and implanta-tion of gel-filled nerve guidance channels. Methods inMolecular Medicine,18, in Morgan JR, Yarmush ML. Tissueengineering methods and protocols", pp.101~119 (1999), Humana PressInc
  14. "Functional recovery following nerve injury and repairby silicon tubulization comparison of laminin-fibronectin", 26, pp.117~122 (1999)
  15. "Two-dimensional versus three-dimen-sional cell counting:a practical perspective", 24, pp.11~17 (2001)
  16. "Three-dimensional counting:an ac-curate and direct method to estimate numbers of cells insectioned material", 278, pp.344~352 (1988)
  17. "Density dependent regulation of hu-man Schwann cell proliferation", 30, pp.165~177 (2000)
  18. "Improved method forharvesting human Schwann cells from mature peripheralnerve and expansion in vitro", 17, pp.327~338 (1996)
  19. "Successfully culturingSchwann cells from adult peripheral nerve", 30, pp.15~19 (1998)
  20. "Characterization of the technique involved inisolating Schwann cells from adult human peripheralnerve", 68, pp.21~26 (1996)
  21. "Viability of cultured nerve grafts:an assessment of pro-liferation of Schwann cells and fibroblasts", 19, pp.356~363 (1999)
  22. "Influence of collagen andlaminin gels concentration on nerve regeneration after re-section and tube repair", 149, pp.243~252 (1998)
  23. "A Schwann cell-seeded intrinsic framework and its satisfactory biocompatibility for a bioartificial nerve graft", 21, pp.6~11 (2001)
  24. "Isolation and serum-free culture of primary Schwann cells from human fetal peripheral nerve", 158, pp.1~8 (1999)
  25. "In vivo predegeneration of peripheral nerves:an effective technique to obtain activated Schwann cells for nerve conduits", 89, pp.17~24 (1999)

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