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가토에서 자가유래 골아줄기세포를 이용한 상악동 골 이식술시 비계체로서 Bio-Oss.의 효과에 관한 연구

The effects of Bio-Oss. as a scaffolds during sinus bone graft using mesenchymal stem cells in rabbit

김은철|최재영|장재덕|차수련|이준|성대혁|최성림
대한구강악안면외과학회 (Korean Association Of Oral And Maxillofacial Surgeons)|2007.10|대한구강악안면외과학회지 (Journal of the Korean Association of Oral and Maxillofacial Surgeons)|제33권 제5호|pp. 405-418

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참고문헌

주제분류치의학

초록

Abstract

Mesenchymal stem cells (MSCs) have been though to be multipotent cells that can replicate that have the potential to differentiate into lineages of mesenchymal tissue including the bone, cartilage, fat, tendon, muscle, and marrow stroma. Especially, scaffolds to support cell-based tissue engineering are critical determinants of clinical efforts to regenerate and repair the body. Selection of a matrix carrier imvolves consideration of the matrix’s role as a scaffold for physical support and host tissue integration as well as its ability to support of synergize the osteoinductive program of the implanted mesenchymal stem cell. The aim of this study is to evaluate the effect of autobone and Bio-Oss. to adherent mesenchymal stem cells as scaffolds on sinus augmentation with fibrin glue mixture in a rabbit model. 16 New Zealand White rabbits were divided randomly into 4 groups based on their time of sacrifice (1, 2, 4 and 8 weeks). First, mesenchymal stem cells were isolated from iliac crest marrow of rabbits and expanded in vitro. Cell culture was performed in accordance with the technique described by Tsutsumi et al. In the present study, the animals were sacrificed at 1, 2, 4 and 8 weeks after transplantation, and the bone formation ability of each sides was evaluated clinically, radiologically, histologically and histomorphologically. According to the histological observations, autobone scaffolds group showed integrated graft bone with host bone from sinus wall. At 2 and 4 weeks, it showed active newly formed bone and neovascularization. At 8 weeks, lamellae bone was observed in sinus graft material area. Radiologically, autobone with stem cell showed more radiopaque than Bio-Oss. scaffolds group. there were significant differences in bone volume between 4 and 8 weeks (p<0.05).

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관련 논문AI추천

가토 상악동에 이식된 β-TCP의 골치유에 관한 실험적 연구마평수, 박정하, 박창주, 백승삼, 심광섭, 최용수, 황경균대한구강악안면외과학회 · 대한구강악안면외과학회지2006 · 제32권 제2호자가골과 BioOss.를 이용한 상악동골이식술: 자가골 함량에 따른 치유 비교김수관, 김영균, 윤필영, 임성철대한구강악안면외과학회 · 대한구강악안면외과학회지2007 · 제33권 제6호가토 두개골 결손부에서 비흡수성 차단막의 유지 기간에 따른 골조직 형성효과김병옥, 장현선, 정민구대한치주과학회 · Journal of Periodontal & Implant Science2007 · 제37권 제3호상악동거상술시 자가골 복합이식이 골재생에 미치는 영향허익, 권영혁, 나의성, 박준봉대한치주과학회 · Journal of Periodontal & Implant Science2004 · 제34권 제2호가토 두개골에서 β-TCP와 자가골 이식에 관한 실험적 연구백승삼, 송승일, 심광섭, 이성훈, 한지영, 황경균대한구강악안면외과학회 · 대한구강악안면외과학회지2004 · 제30권 제4호가토의 두개골 결손부에서 골재생에 끼치는 골막의 역할김병옥, 김상목, 박주철, 장현선대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제4호

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