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정상 치은 조직에서 배양된 각화세포에서 Cyclosporine A에 의한 MMP-1, TIMP-1, TGF-β1의 발현에 관한 연구

The mRNA expression of MMP-1, TIMP-1, TGF-β1 in gingival keratocytes from gingival hyperplasia induced by cyclosporine A

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Cyclosporine A (CsA) is a versatile immunosuppresive agent used to prevent graft rejection syndrome and treat autoimmune disease. One of the major side effects associated with CsA is the abnormal gingival hyperplasia. The purpose of this study was to investigate the relationship between the mRNA expression of the MMP-1, TIMP-1, and TGF-β1 and the concentration of CsA in cultured human gingival keratinocytes. Materials & Methods : Gingival keratocytes were obtained from gingival tissues of 4 healthy donors. The cultured gingival keratocytes were incubated with increasing concentrations of CsA (0-2000 ng/ml) for 24 hours and the expression of MMP-1, TIMP-1, and TGF-β1 were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results : The expressions of MMP-1 and TGF-β1 were not significantly different according to the concentrations of CsA. The expression of TIMP-1 was significantly increased at the CsA concentration of 500 ng/ml. Conclusion : We concluded that the gingival hyperplasia induced by CsA was more related with TIMP-1 than MMP-1 or TGF-β1 on gingival collagen metabolism in patients treated with CsA.

Abstract

Cyclosporine A (CsA) is a versatile immunosuppresive agent used to prevent graft rejection syndrome and treat autoimmune disease. One of the major side effects associated with CsA is the abnormal gingival hyperplasia. The purpose of this study was to investigate the relationship between the mRNA expression of the MMP-1, TIMP-1, and TGF-β1 and the concentration of CsA in cultured human gingival keratinocytes. Materials & Methods : Gingival keratocytes were obtained from gingival tissues of 4 healthy donors. The cultured gingival keratocytes were incubated with increasing concentrations of CsA (0-2000 ng/ml) for 24 hours and the expression of MMP-1, TIMP-1, and TGF-β1 were determined by reverse transcription-polymerase chain reaction (RT-PCR). Results : The expressions of MMP-1 and TGF-β1 were not significantly different according to the concentrations of CsA. The expression of TIMP-1 was significantly increased at the CsA concentration of 500 ng/ml. Conclusion : We concluded that the gingival hyperplasia induced by CsA was more related with TIMP-1 than MMP-1 or TGF-β1 on gingival collagen metabolism in patients treated with CsA.

참고문헌 (36)

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관련 논문AI추천

Cyclosporin A-induced Gingival Overgrowth is Closely Associated with Regulation Collagen Synthesis by the Beta Subunit of Prolyl 4-hydroxylase and Collagen Degradation by Testican 1- mediated Matrix Metalloproteinase- 2 Expression김재연, 김현정, 박광균, 박성희, 정원윤, 조규성, 최성호대한구강생물학회 · International Journal of Oral Biology2008 · 제33권 제4호A Novel Method to Study the Effects of Cyclosporine on Gingival Overgrowth in Children김종수, 한금아대한소아치과학회 · 대한소아치과학회지2018 · 제45권 제3호Azithromycin이 Cyclosporin-A에 의한 치은섬유아세포 과증식에 미치는 영향에 대한 in vitro 연구노현수, 박광균, 정원윤, 조규성, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제4호Cyclosporin A에 의한 치은 증식증에서 FGF-5와 FGF-7의 발현 양상에 대한 연구김성곤, 남동석, 윤경인, 정미향대한구강악안면외과학회 · 대한구강악안면외과학회지2006 · 제32권 제3호사람 치은 섬유모세포에서 Cyclosporin-A 유도 세포증식에 대한 항세포고사 기전김원재, 정태술, 정현주대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제3호CsA Affects the Rat Submandibular Glands via Regulating the CypA Expression김민석, 김선헌, 유홍일, 이은주, Jee-Hae Kang, So-Young Yang, Young-Gil Hong대한구강생물학회 · International Journal of Oral Biology2012 · 제37권 제4호

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