골막기원세포에서 발현되는 혈관내피세포성장인자 관련 자가성장
VEGF-RELATED AUTOCRINE GROWTH IN PERIOSTEAL-DERIVED CELLS
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periostealderived cells. Materials and methods: Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. Results: The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells. Conclusion: These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.
Abstract
Purpose: The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periostealderived cells. Materials and methods: Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. Results: The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells. Conclusion: These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.
참고문헌 (18)
- Mayer H, "Vascular endothelial growth factor (VEGF-A) expression in human mesenchymal stem cells: autocrine and paracrine role on osteoblastic and endothelial differentiation", J Cell Biochem, 95, pp.827~839 (2005)
- Neufeld G, "Gengrinovitch S, Poltorak Z. Vascular endothelial growth factor (VEGF) and its receptors", FASEB J, 19, pp.9~22 (1999)
- 박봉욱, "배양된 인간 골막기원세포의 조골세포 분화과정에서 골기질 형성정도와 혈관내피세포 성장인자 신호와의 상관관계", 대한악안면성형재건외과학회지, 29(3), pp.197~205 (2007), 대한악안면성형재건외과학회
- 박봉욱, "고용량의 Dexamethasone 존재하에서 골막기원세포에서 발현되는 혈관신생인자의 평가", 대한악안면성형재건외과학회지, 30(3), pp.217~224 (2008), 대한악안면성형재건외과학회
- Masood R, "Vascular endothelial growth factor (VEGF) is an autocrine growth factor for VEGF receptor-positive human tumors", Blood, 98, pp.1904~1913 (2001)
- Strizzi L, "Vascular endothelial growth factor is an autocrine growth factor in human malignant mesothelioma", J Pathol, 193, pp.468~475 (2001)
- Tian X, "Vascular endothelial growth factor: acting as an autocrine growth factor for human gastric adenocarcinoma cell MGC803", Biochem Biophys Res Commun, 286, pp.505~512 (2001)
- 박봉욱, "배양된 인간 골막기원세포의 조골활성 및 골기질 형성의 평가", 대한악안면성형재건외과학회지, 28(6), pp.511~519 (2006), 대한악안면성형재건외과학회
- Furumatsu T, "Vascular endothelial growh factor prinicipally acts as the main angiogenic factor in the early stage of human osteoblastogenesis", J Biochem, 133, pp.633~639 (2003)
- Gerber HP, "VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation", Nat Med, 5, pp.623~628 (1999)
- Maes C, "Impaired angiogenesis and endochondral bone formation in mice lacking the vascular endothelial growth factor isoforms VEGF164 and VEGF188", Mech Dev, 111, pp.61~73 (2002)
- Wang DS, "Anabolic effects of 1,25-dihydroxyvitamin D3 on osteoblasts are enhanced by vascular endothelial growth factor produced by osteoblasts and by growth factors produced by endothelial cells", Endocrinology, 138, pp.2953~2962 (1997)
- Gee MF, "Vascular endothelial growth factor acts in an autocrine manner in rhabdomyosarcoma cell lines and can be inhibited with all-trans-retinoic acid", Oncogene, 24, pp.8025~8037 (2005)
- Millanta F, "The role of vascular endothelial growth factor and its receptor Flk-1", KDR in promoting tumour angiogenesis in feline and canine mammary carcinomas: a preliminary study of autocrine and paracrine loops, 2006, pp.81, 350~357
- Weigand M, "Autocrine vascular endothelial growth factor signalling in breast cancer. Evidence from cell lines and primary breast cancer cultures in vitro", Angiogenesis, 8, pp.197~204 (2005)
- 김종렬, "배양된 인간 골막기원세포의 조골활성에 대한 덱사메타손 농도의 효과", 대한악안면성형재건외과학회지, 31(4), pp.287~293 (2009), 대한악안면성형재건외과학회
- Haper J, "Neuropilin-1 expression in osteogenic cells: down-regulation during differentiation of osteoblasts into osteocytes", J Cell Biochem, 81, pp.82~92 (2001)
- Tao O, "Characterization of a new alternatively spliced neuropilin-1 isoform", Angiogenesis, 6, pp.39~45 (2003)