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MG-63 세포주에서 Vascular Endothelial Growth Factor (VEGF) mRNA 발현에 대한Insulin-like Growth Factor I (IGF-I)의 효과에 대한 연구

The effects of insulin-like Growth Factor I (IGF-I) on expression of vascular endothelial growth factor (VEGF) mrna in MG-63 osteoblastlike cells

강나라|명훈|정필훈|서제덕
대한구강악안면외과학회 (Korean Association Of Oral And Maxillofacial Surgeons)|2005.10|대한구강악안면외과학회지 (Journal of the Korean Association of Oral and Maxillofacial Surgeons)|제31권 제5호|pp. 363-369

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초록

Abstract

Purpose: To determine the role of Insulin-like Growth Factor-I (IGF-I) in the regulation of Vascular Endothelial Growth Factor (VEGF) expression in MG-63 cells and then to find the mechanism b which this regulation occurs. Materials and methods: MG-63 cells were grown to confluence in 60-mm dishes. To determine the effects of IGF-I on expression of VEGF mRNA according to time and concentration, the cells were treated with 10 nM IGF-I, following isolation of total RNA and Northern blot analysis after 1, 2, 4, 8, 12, 24 hours and after 2 hours of treatment with 0.5, 2, 10, 25, 50 nM IGF-I respectively, isolation of total RNA and Northern blot analysis were followed. To determine the mechanism of action of IGF-I, inhibitors such as hydroxyurea (76.1 ㎍/㎖), actinomycin D (2.5 ㎍/㎖), cycloheximide (10 ㎍/㎖) were added 1 hour after treatment of 10 nM IGF-I. Results: 1. the expression of VEGF mRNA was increased with treatment of IGF-I. 2. The expression of VEGF mRNA was increased according to time- and concentration dependent manner of IGF-I. 3. The effect of IGF-I was decreased by hydroxyuera, actinomycin D, but not by cycloheximide. Conclusion: IGF-I regulate the expression of VEGF mRNA in the level of DNA synthesis and transcription. These results could suggest that IGF-I plays an important role in angiogenesis in the process of new bone formation and remodeling.

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