Effects of fresh mineralized dentin and cementum on socket healing: a preliminary study in dogs
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Objectives: Dentin is composed of many minerals and growth factors. Based on this composition, we studied its effect as a possible regenerative ma-terial for alveolar healing.Materials and Methods: This study was conducted using four 2.5-year-old mongrel dogs (male; weight, 25 to 30 kg). The third mandibular premo-lars were carefully mobilized with a dental elevator and then removed using forceps. The crown portions of the extracted teeth were removed with cut-ters, and the root portions of the remaining teeth were collectively trimmed as closely as possible to 350 to 500 μm. Dentin and cementum (DC) chips harvested from the extracted teeth were soaked in blood and packed into the fresh sockets (autograft). Biopsies were performed at the ends of day 14 and day 56 following implantation. Data were expressed as mean±standard deviation and compared with t-test results.Results: The ratio of SA(bone) to total area of each probe was determined and was 170±16 μm2 for the control group and 71±14 μm2 for the DC group, a significant diference (P<0.05). Conclusion: DC particulate grafts offered no improvement in bone regeneration in alveolar extraction sockets.
Abstract
Objectives: Dentin is composed of many minerals and growth factors. Based on this composition, we studied its effect as a possible regenerative ma-terial for alveolar healing.Materials and Methods: This study was conducted using four 2.5-year-old mongrel dogs (male; weight, 25 to 30 kg). The third mandibular premo-lars were carefully mobilized with a dental elevator and then removed using forceps. The crown portions of the extracted teeth were removed with cut-ters, and the root portions of the remaining teeth were collectively trimmed as closely as possible to 350 to 500 μm. Dentin and cementum (DC) chips harvested from the extracted teeth were soaked in blood and packed into the fresh sockets (autograft). Biopsies were performed at the ends of day 14 and day 56 following implantation. Data were expressed as mean±standard deviation and compared with t-test results.Results: The ratio of SA(bone) to total area of each probe was determined and was 170±16 μm2 for the control group and 71±14 μm2 for the DC group, a significant diference (P<0.05). Conclusion: DC particulate grafts offered no improvement in bone regeneration in alveolar extraction sockets.
참고문헌 (26)
- Irinakis T, "Rationale for socket preservation after extraction of a single-rooted tooth when planning for future implant placement", J Can Dent Assoc, 72, pp.917~922 (2006)
- Botticelli D, "Hard-tissue alterations following immediate implant placement in extraction sites", J Clin Periodontol, 31, pp.820~828 (2004)
- Tan WL, "systematic review of post-extractional alveolar hard and soft tissue dimensional changes in humans", Clin Oral Implants Res, 23(Suppl 5), pp.1~21 (2012)
- Dimova C, "Socket preservation procedure after tooth extraction", Key Engineering Materials, 587, pp.325~330 (2014)
- Vignoletti F, "Surgical protocols for ridge preservation after tooth extraction. A systematic review", Clin Oral Implants Res, 23(Suppl 5), pp.22~38 (2012)
- Sattayasanskul W, "Dense hydroxyapatite root replica implantation: measurement of mandibular ridge preservation", Int J Oral Maxillofac Implants, 3, pp.203~207 (1988)
- Jung RE, "Postextraction tissue management: a soft tissue punch technique", Int J Periodontics Restorative Dent, 24, pp.545~553 (2004)
- Kim YK, "Analysis of the inorganic component of autogenous tooth bone graft material", J Nanosci Nanotechnol, 11, pp.7442~7445 (2011)
- Huggins C, "Transformation of fibroblasts by allogeneic and xenogeneic transplants of demineralized tooth and bone", J Exp Med, 132, pp.1250~1258 (1970)
- Finkelman RD, "Quantitation of growth factors IGF-I, SGF", IGF-II, and TGF-beta in human dentin, 1990, pp.5, 717~723
- Bang G, "Bone induction in excavation chambers in matrix of decalcified dentin", Arch Surg, 94, pp.781~789 (1967)
- Bessho K, "Purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction", J Oral Maxillofac Surg, 48, pp.162~169 (1990)
- Butler WT, "Noncollagenous proteins of a rat dentin matrix possessing bone morphogenetic activity", J Dent Res, 56, pp.228~232 (1977)
- Murata M, "Human acid-insoluble dentin with BMP2 accelerates bone induction in subcutaneous and intramuscular tissues", J Ceram Soc Jpn, 118, pp.438~441 (2010)
- Yeomans JD, "Bone induction by decalcified dentine implanted into oral, osseous and muscle tissues", Arch Oral Biol, 12, pp.999~1008 (1967)
- Murata M, "Bone engineering using human demineralized dentin matrix and recombinant human BMP2", J Hard Tissue Biol, 14, pp.80~81 (2005)
- Reed MG, "One-stop stereology: the estimation of 3D parameters using isotropic rulers", J Microsc, 239, pp.54~65 (2010)
- Wang X, "Cancellous bone lamellae strongly affect microcrack propagation and apparent mechanical properties: separation of patients with osteoporotic fracture from normal controls using a 2D nonlinear finite element method (biomechanical stereology)", Bone, 42, pp.1184~1192 (2008)
- Lewandrowski KU, "Improved osteoinduction of cortical bone allografts:a study of the effects of laser perforation and partial demineralization", J Orthop Res, 15, pp.748~756 (1997)
- Reddi AH, "Bone matrix in the solid state: geometric influence on differentiation of fibroblasts", Adv Biol Med Phys, 15, pp.1~18 (1974)
- Kim YK, "Development of a novel bone grafting material using autogenous teeth", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 109, pp.496~503 (2010)
- Nampo T, "A new method for alveolar bone repair using extracted teeth for the graft material", J Periodontol, 81, pp.1264~1272 (2010)
- 정혜린, "미니피그에서 자가치아뼈 이식의 골형성 효과에 대한 연구", 대한구강악안면외과학회지, 37(5), pp.375~379 (2011), 대한구강악안면외과학회
- Devecioğlu D, "Biomaterials in periodontal regenerative surgery: effects of cryopreserved bone, commercially available coral, demineralized freeze-dried dentin, and cementum on periodontal ligament fibroblasts and osteoblasts", J Biomater Appl, 19, pp.107~120 (2004)
- Gomes MF, "Optical density of bone repair after implantation of homogenous demineralized dentin matrix in diabetic rabbits", Braz Oral Res, 22, pp.275~280 (2008)
- Urist MR, "Transmembrane bone morphogenesis across multiple-walled diffusion chambers. New evidence for a diffusible bone morphogenetic property", Arch Surg, 112, pp.612~619 (1977)