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Group B Streptococcus Colonization, Antibiotic Susceptibility, and Serotype Distribution among Saudi Pregnant Women

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Background: Group B Streptococcus (GBS) comprises the normal flora of the female urogenital tract and can be transferred to neonates during delivery, causing invasive diseases. This study was performed to investigate the colonization rate, antibiotic susceptibility, and serotype of GBS among Saudi pregnant women. Materials and Methods: In this cross-sectional study, vagino-rectal swabs from 400 pregnant women were collected over a period of one year. Identification of GBS isolates and determination of their antibiotic susceptibility were performed using the Microscan Walk Away system. The isolates were then typed using both latex agglutination and capsular genebased multiplex polymerase chain reaction assays. Results: Sixty (15.0%) subjects were colonized by GBS, with serotype Ia as the dominant type (30.0%) followed by serotype III and V (25.0%, each). Only 43 (71.7%) isolates were typed by latex agglutination, whereas the remaining isolates were not typable or were non-specifically typed as compared to the genotyping assay, which revealed the specific type of each GBS isolate. The highest resistance rates were observed for erythromycin and clindamycin (16.7%, each), which were mainly restricted to the prevalent serotypes. Conclusion: This study is the first to report the distribution of GBS serotypes based on molecular genotyping in Saudi Arabia. GBS colonization was evident among pregnant women, and resistance to erythromycin and clindamycin was predominant among serotypes Ia, III, and V. Molecular genotyping using capsular gene-based multiplex PCR provided reliable typing of the investigated GBS isolates in terms of sensitivity and specificity as compared to conventional serotyping using latex agglutination.

Abstract

Background: Group B Streptococcus (GBS) comprises the normal flora of the female urogenital tract and can be transferred to neonates during delivery, causing invasive diseases. This study was performed to investigate the colonization rate, antibiotic susceptibility, and serotype of GBS among Saudi pregnant women. Materials and Methods: In this cross-sectional study, vagino-rectal swabs from 400 pregnant women were collected over a period of one year. Identification of GBS isolates and determination of their antibiotic susceptibility were performed using the Microscan Walk Away system. The isolates were then typed using both latex agglutination and capsular genebased multiplex polymerase chain reaction assays. Results: Sixty (15.0%) subjects were colonized by GBS, with serotype Ia as the dominant type (30.0%) followed by serotype III and V (25.0%, each). Only 43 (71.7%) isolates were typed by latex agglutination, whereas the remaining isolates were not typable or were non-specifically typed as compared to the genotyping assay, which revealed the specific type of each GBS isolate. The highest resistance rates were observed for erythromycin and clindamycin (16.7%, each), which were mainly restricted to the prevalent serotypes. Conclusion: This study is the first to report the distribution of GBS serotypes based on molecular genotyping in Saudi Arabia. GBS colonization was evident among pregnant women, and resistance to erythromycin and clindamycin was predominant among serotypes Ia, III, and V. Molecular genotyping using capsular gene-based multiplex PCR provided reliable typing of the investigated GBS isolates in terms of sensitivity and specificity as compared to conventional serotyping using latex agglutination.

참고문헌 (40)

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관련 논문AI추천

Recent Epidemiological Changes in Group B Streptococcus Among Pregnant Korean Women강지은, 최성진, Uh Young대한진단검사의학회 · Annals of Laboratory Medicine2021 · 제41권 제4호A Retrospective National Study on Colonization Rate and Antimicrobial Susceptibility of Streptococcus agalactiae in Pregnant Korean Women, 2018–2020어영, 김영진, 배혜경, 이경렬, 이경원, 최성진, 홍정미연세대학교의과대학 · Yonsei Medical Journal2022 · 제63권 제8호Risk Factors Associated with Group B Streptococcus Resistant to Clindamycin and Erythromycin in Pregnant Korean Women기모란, 김문영, 김은주, 류현미, 신정환, 양재혁, 오관영, 옥지형, Betsy Foxman대한감염학회 · Infection and Chemotherapy2013 · 제45권 제3호임신부의 Group B Streptococci 보균율, 혈청형 및 Macrolide-Lincosamide-StreptograminB 내성형의 분포어영, 권오건, 윤갑준, 이관수, 장인호, 조현미, 최성진대한임상미생물학회 · Annals of Clinical Microbiology2009 · 제12권 제4호Usefulness of a Rapid Real-time PCR Assay in Prenatal Screening for Group B Streptococcus Colonization김영문, 김의종, 박성섭, 박정수, 성문우, 신손문, 양재혁, 조동희대한진단검사의학회 · Annals of Laboratory Medicine2013 · 제33권 제1호Prevalence of group B streptococcus colonization in pregnant women in a tertiary care center in Korea김기태, 김다현, 김영남, 민병진, 변정미, 성문수, 이경복, 정대훈, 정은정대한산부인과학회 · Obstetrics & Gynecology Science2018 · 제61권 제5호

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