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다중시설을 이용한 시민의 손에서 분리된 황색포도알균의 항생제 내성 및 메티실린 내성 황색포도알균의 분자 역학적 특성

Profile of Antimicrobial Resistance of Staphylococcus aureus and Molecular Epidemiologic Characterization of Methicillin-resistant Staphylococcus aureus (MRSA) Isolated from Hands of People Using Multitude Facilities

김선희|김민지|김은선|문용운|서진종|김태순|기혜영|류필열|하동룡
대한감염학회 (The Korean Society Of Infectious Diseases)|2012.08|Infection and Chemotherapy|제44권 제4호|pp. 289-298

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Antimicrobial resistanceEnterotoxinMultilocus sequence typingMethicillin-resistant S. aureusSCCmectypePanton-Valentine Leukocidin
주제분류내과학

초록

Background: The purpose of this study was to investigate perception of hand hygiene and actual hand washing practices of people who used public facilities as well as the presence of indicator bacteria and food-borne pathogens on their hands. Data from this study will be used as a tool for public education and provide basic information on the potential risk for the spread of infectious disease by hands. Materials and Methods: Sixty S. aureus and 15 methicillin-resistant S. aureus (MRSA)were recovered from 500 swab samples from hands of people in public places, including super markets and amusement facilities in Gwangju Metropolitan City during February to May 2011. Using conventional methods and the Vitek system, all of the isolates were confirmed as Staphylococcus auerus (S. aureus). Antimicrobial susceptibility was determined by performing disk diffusion testing according to the Clinical Laboratory Standard Institute guidelines. The minimum inhibition concentrations (MICs) of MRSA isolates were tested using E-test strips. To confirm the MRSA, polymerase chain reaction (PCR) for the S.aureus-specific gene and mecA gene was performed. Gene detection using PCR, SCCmec typing, Panton-Valentine Leukocidin (PVL), and Multilocus sequence typing (MLST) were performed on all isolates of MRSA. Results: Of 60 S. aureus isolates, 48 (80%) harbored at least one type of enterotoxin gene: two, three, four, and five types of enterotoxin gene were found in 16 (26.7%),seven (11.7%), 10 (16.7%), and eight (13.3%) isolates, respectively. The most prevalent antimicrobial resistance observed in the S. aureus isolates was to penicillin (92%, 55/60),followed by erythromycin (35%, 21/60), oxacillin (32%, 19/60), and ampicillin (23%,14/60). No resistance was observed against vancomycin, clindamycin, linazolid, rifampin,imipenem, trimethoprim/sulfamethoxazole, and telithromycin. In this study, based on molecular characterization of MRSA isolates, all MRSA, except for one isolate, belonged to ST72 and SCCmec type IV. Eleven of 15 (78.6%) MRSA were ST72:SCCmecIV:t324. Conclusions: In this study, we detected serious pathogens, including S. aureus and MRSA, from swab samples of peoples’ hands. Most S. aureus isolates harbored the enterotoxin gene and the types of MRSA isolated in this study were communityassociated MRSA, indicating the importance of washing hands for public health.

Abstract

Background: The purpose of this study was to investigate perception of hand hygiene and actual hand washing practices of people who used public facilities as well as the presence of indicator bacteria and food-borne pathogens on their hands. Data from this study will be used as a tool for public education and provide basic information on the potential risk for the spread of infectious disease by hands. Materials and Methods: Sixty S. aureus and 15 methicillin-resistant S. aureus (MRSA)were recovered from 500 swab samples from hands of people in public places, including super markets and amusement facilities in Gwangju Metropolitan City during February to May 2011. Using conventional methods and the Vitek system, all of the isolates were confirmed as Staphylococcus auerus (S. aureus). Antimicrobial susceptibility was determined by performing disk diffusion testing according to the Clinical Laboratory Standard Institute guidelines. The minimum inhibition concentrations (MICs) of MRSA isolates were tested using E-test strips. To confirm the MRSA, polymerase chain reaction (PCR) for the S.aureus-specific gene and mecA gene was performed. Gene detection using PCR, SCCmec typing, Panton-Valentine Leukocidin (PVL), and Multilocus sequence typing (MLST) were performed on all isolates of MRSA. Results: Of 60 S. aureus isolates, 48 (80%) harbored at least one type of enterotoxin gene: two, three, four, and five types of enterotoxin gene were found in 16 (26.7%),seven (11.7%), 10 (16.7%), and eight (13.3%) isolates, respectively. The most prevalent antimicrobial resistance observed in the S. aureus isolates was to penicillin (92%, 55/60),followed by erythromycin (35%, 21/60), oxacillin (32%, 19/60), and ampicillin (23%,14/60). No resistance was observed against vancomycin, clindamycin, linazolid, rifampin,imipenem, trimethoprim/sulfamethoxazole, and telithromycin. In this study, based on molecular characterization of MRSA isolates, all MRSA, except for one isolate, belonged to ST72 and SCCmec type IV. Eleven of 15 (78.6%) MRSA were ST72:SCCmecIV:t324. Conclusions: In this study, we detected serious pathogens, including S. aureus and MRSA, from swab samples of peoples’ hands. Most S. aureus isolates harbored the enterotoxin gene and the types of MRSA isolated in this study were communityassociated MRSA, indicating the importance of washing hands for public health.

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