High In Vitro Infectivity of a Doxycycline-Insensitive Strain of Orientia tsutsugamushi
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We compared the infectivity and growth rates of 12 strains of Orientia tsutsugamushi in a cell culture system. ECV304 cellswere infected with O. tsutsugamushi strains for 4 hr, and the culture was maintained for 72 hr. Immunofluorescence (IF) stainingwas performed at 4, 24, 48, and 72 hr after inoculation (hpi), and IF-positive foci were enumerated. The AFSC-4 strain, which isknown to be insensitive to doxycycline, showed higher numbers of IF-positive foci than the other 11 strains at 4, 24, 48, and 72hpi, which may explain its apparent insensitivity to antibiotics.
Abstract
We compared the infectivity and growth rates of 12 strains of Orientia tsutsugamushi in a cell culture system. ECV304 cellswere infected with O. tsutsugamushi strains for 4 hr, and the culture was maintained for 72 hr. Immunofluorescence (IF) stainingwas performed at 4, 24, 48, and 72 hr after inoculation (hpi), and IF-positive foci were enumerated. The AFSC-4 strain, which isknown to be insensitive to doxycycline, showed higher numbers of IF-positive foci than the other 11 strains at 4, 24, 48, and 72hpi, which may explain its apparent insensitivity to antibiotics.
참고문헌 (13)
- 정문현, "Persistence of Orientia tsutsugamushi in Humans", Journal of Korean Medical Science, 27(3), pp.231~235 (2012), 대한의학회
- Rajapakse S, "Drug treatment ofscrub typhus", Trop Doct, 41, pp.1~4 (2011)
- Watt G, "Scrub typhus infections poorly responsive toantibiotics in northern Thailand", Lancet, 348, pp.86~89 (1996)
- Watt G, "Azithromycin activities against Orientiatsutsugamushi strains isolated in cases of scrub typhusin Northern Thailand", Antimicrob Agents Chemother, 43, pp.2817~2818 (1999)
- Strickman D, "In vitro effectiveness of azithromycinagainst doxycycline-resistant and -susceptible strains ofRickettsia tsutsugamushi, etiologic agent of scrub typhus", Antimicrob Agents Chemother, 39, pp.2406~2410 (1995)
- Kelly DJ, "Scrub typhus:the geographic distribution of phenotypic and genotypicvariants of Orientia tsutsugamushi", Clin InfectDis, 48(3), pp.S203~S230 (2009)
- Mi Jeong Kim, "Improved Antibiotic Susceptibility Test of Orientia tsutsugamushi by Flow Cytometry Using Monoclonal Antibody", Journal of Korean Medical Science, 22(1), pp.1~6 (2007), 대한의학회
- Kelly DJ, "Rickettsiatsutsugamushi infection in cell culture: antibiotic susceptibilitydetermined by flow cytometry", Am J TropMed Hyg, 53, pp.602~606 (1995)
- Groves MG, "Characterization of factors determiningRickettsia tsutsugamushi pathogenicity for mice", Infect Immun, 57, pp.1476~1482 (1989)
- 김은실, "국내 환자에서 분리된 Orientia tsutsugamushi에서 doxycycline 내성", Infection and Chemotherapy, 40(5), pp.259~265 (2008), 대한감염학회
- 정혜원, "Phylogenetic Analysis of the 56-kDa Type-Specific Protein Genes of Orientia tsutsugamushi in Central Korea", Journal of Korean Medical Science, 27(11), pp.1315~1319 (2012), 대한의학회
- Watt G, "Doxycycline and rifampicinfor mild scrub-typhus infections in northern Thailand:a randomised trial", Lancet, 356, pp.1057~1061 (2000)
- Kim YS, "Controlled trial of doxycycline versus rifampin fortreatment of mild to moderate scrub typhus", 석사, pp.2010