The Spread of Insertion Sequences Element and Transposons in Carbapenem Resistant Acinetobacter baumannii in a Hospital Setting in Southwestern Iran
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Acinetobacter baumannii is one of the most important hospital pathogenic bacteria that cause infectious diseases. The present study aimed to determine the frequency of carbapenem resistance genes in association with transposable elements and molecular typing of carbapenem-resistant A. baumannii bacteria collected from patients in Shiraz, Iran. Materials and methods: A total of 170 carbapenem-resistant A. baumannii isolates were obtained from different clinical specimens in two hospitals. The minimum inhibitory concentrations (MIC) of imipenem were determined and the prevalence of OXA Carbapenemases, Metallo-β-lactamases genes, insertion sequences (IS) elements, and transposons were evaluated by the polymerase chain reaction (PCR) method. Finally, molecular typing of the isolates was performed by the Enterobacterial Repetitive Intergenic Consensus-PCR method. Results: The MICs ranged from 16 to 1,024 µg/mL for imipenem-resistant A. baumannii isolates. Out of the 170 carbapenem resistant A. baumannii isolates, blaOXA-24-like (94, 55.3%) followed by blaOXA-23-like (71, 41.7%) were predominant. In addition, A. baumannii isolates carried blaVIM (71, 41.7%), blaGES (32, 18.8%), blaSPM (4, 2.3%), and blaKPC (1, 0.6%). Moreover, ISAba1 (94.2%) and Tn2009 (39.2%) were the most frequent transposable elements. Furthermore, (71, 44.0%) and (161, 94.7%) of the ISAba1 of the isolates were associated with blaOXA-23 and blaOXA-51 genes, respectively. Besides (3, 1.7%), (1, 0.6%) and (5, 2.9%) of blaOXA-23 were associated with IS18, ISAba4, and ISAba2, respectively. Considering an 80.0% cut off, clusters and four singletons were detected. Conclusion: According to the results, transposable elements played an important role in the development of resistance genes and resistance to carbapenems. The results also indicated carbapenem-resistant A. baumannii bacteria as a public health concern.
Abstract
Background: Acinetobacter baumannii is one of the most important hospital pathogenic bacteria that cause infectious diseases. The present study aimed to determine the frequency of carbapenem resistance genes in association with transposable elements and molecular typing of carbapenem-resistant A. baumannii bacteria collected from patients in Shiraz, Iran. Materials and methods: A total of 170 carbapenem-resistant A. baumannii isolates were obtained from different clinical specimens in two hospitals. The minimum inhibitory concentrations (MIC) of imipenem were determined and the prevalence of OXA Carbapenemases, Metallo-β-lactamases genes, insertion sequences (IS) elements, and transposons were evaluated by the polymerase chain reaction (PCR) method. Finally, molecular typing of the isolates was performed by the Enterobacterial Repetitive Intergenic Consensus-PCR method. Results: The MICs ranged from 16 to 1,024 µg/mL for imipenem-resistant A. baumannii isolates. Out of the 170 carbapenem resistant A. baumannii isolates, blaOXA-24-like (94, 55.3%) followed by blaOXA-23-like (71, 41.7%) were predominant. In addition, A. baumannii isolates carried blaVIM (71, 41.7%), blaGES (32, 18.8%), blaSPM (4, 2.3%), and blaKPC (1, 0.6%). Moreover, ISAba1 (94.2%) and Tn2009 (39.2%) were the most frequent transposable elements. Furthermore, (71, 44.0%) and (161, 94.7%) of the ISAba1 of the isolates were associated with blaOXA-23 and blaOXA-51 genes, respectively. Besides (3, 1.7%), (1, 0.6%) and (5, 2.9%) of blaOXA-23 were associated with IS18, ISAba4, and ISAba2, respectively. Considering an 80.0% cut off, clusters and four singletons were detected. Conclusion: According to the results, transposable elements played an important role in the development of resistance genes and resistance to carbapenems. The results also indicated carbapenem-resistant A. baumannii bacteria as a public health concern.
참고문헌 (37)
- Jiang L, "Molecular epidemiology and genetic characterisation of carbapenem-resistant Acinetobacter baumannii isolates from Guangdong Province, South China", J Glob Antimicrob Resist, 17, pp.84~89 (2019)
- Hashemi B, "Frequency of metalo beta lactamase genes, bla IMP1, INT 1 in Acinetobacter baumanii isolated from burn patients North of Iran", Gene Reports, 21, pp.100800~ (2020)
- Simo Tchuinte PL, "Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar", Antimicrob Resist Infect Control, 8, pp.31~ (2019)
- Pagano M, "Mobile genetic elements related to carbapenem resistance in Acinetobacter baumannii", Braz J Microbiol, 47, pp.785~792 (2016)
- Mugnier PD, "Worldwide dissemination of the blaOXA-23 carbapenemase gene of Acinetobacter baumannii", Emerg Infect Dis, 16, pp.35~40 (2010)
- Chen Y, "Spread of the blaOXA-23-Containing Tn2008 in Carbapenem-Resistant Acinetobacter baumannii Isolates Grouped in CC92 from China", Front Microbiol, 8, pp.163~ (2017)
- Zhao Y, "Outbreak of carbapenem-resistant Acinetobacter baumannii carrying the carbapenemase OXA-23 in ICU of the eastern Heilongjiang Province, China", BMC Infect Dis, 19, pp.452~ (2019)
- Mahon CR, "Textbook of diagnostic microbiology: e-book" (2018), Elsevier Health Sciences
- Performance standards for antimicrobial susceptibility testing; Twenty-first informational supplement, "Clinical and Laboratory Standards Institute (CLSI)", CLSI, ~, pp.CLSI document M100-S21 (2011)
- Stehling EG, "Molecular typing and biological characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Brazil", Braz J Infect Dis, 14, pp.462~467 (2010)
- Tavakol M, "Genotyping and distribution of putative virulence factors and antibiotic resistance genes of Acinetobacter baumannii strains isolated from raw meat", Antimicrob Resist Infect Control, 7, pp.120~ (2018)
- Nazari M, "Detection of carbapenem resistance and virulence genes among Acinetobacter baumannii isolated from hospital environments in center of Iran", J Curr Biomed Rep, 2, pp.14~ (2021)
- Farshadzadeh Z, "Growth rate and biofilm formation ability of clinical and laboratory-evolved colistin-resistant strains of Acinetobacter baumannii", Front Microbiol, 9, pp.153~ (2018)
- Xie R, "Analysis of global prevalence of antibiotic resistance in Acinetobacter baumannii infections disclosed a faster increase in OECD countries", Emerg Microbes Infect, 7, pp.31~ (2018)
- Coelho JM, "Occurrence of carbapenem-resistant Acinetobacter baumannii clones at multiple hospitals in London and Southeast England", J Clin Microbiol, 44, pp.3623~3627 (2006)
- Rahimi S, "The relationship between antibiotic resistance phenotypes and biofilm formation capacity in clinical isolates of Acinetobacter baumannii", Jundishapur J Microbiol, 11, pp.e74315~ (2018)
- Al-Agamy MH, "Molecular characterization of carbapenem-insensitive Acinetobacter baumannii in Egypt", Int J Infect Dis, 22, pp.49~54 (2014)
- Elabd FM, "Molecular characterization of oxacillinases among carbapenem-resistant Acinetobacter baumannii nosocomial isolates in a Saudi hospital", J Infect Public Health, 8, pp.242~247 (2015)
- Cicek AC, "OXA-and GES-type β-lactamases predominate in extensively drug-resistant Acinetobacter baumannii isolates from a Turkish University Hospital", Clin Microbiol Infect, 20, pp.410~415 (2014)
- Li S, "Molecular characteristics of carbapenem-resistant Acinetobacter spp. from clinical infection samples and fecal survey samples in Southern China", BMC Infect Dis, 19, pp.900~ (2019)
- Vijayakumar S, "Molecular characterization of invasive carbapenem-resistant Acinetobacter baumannii from a tertiary care hospital in South India", Infect Dis Ther, 5, pp.379~387 (2016)
- Alcántar-Curiel MD, "Carbapenem-resistant Acinetobacter baumannii in three tertiary care hospitals in Mexico : virulence profiles, innate immune response and clonal dissemination", Front Microbiol, 10, pp.2116~ (2019)
- Pragasam AK, "Molecular characterisation of antimicrobial resistance in Pseudomonas aeruginosa and Acinetobacter baumannii during 2014 and 2015 collected across India", Indian J Med Microbiol, 34, pp.433~441 (2016)
- Alkasaby NM, "Molecular Study of Acinetobacter baumannii isolates for metallo-β-Lactamases and extended-spectrum-β-lactamases genes in intensive care unit, Mansoura University Hospital, Egypt", Int J Microbiol, 2017, pp.3925868~ (2017)
- Noori M, "High prevalence of metallo-beta-lactamase producing Acinetobacter baumannii isolated from two hospitals of Tehran, Iran", Arch Pediatr Infect Dis, 2, pp.e15439~ (2014)
- Peymani A, "High prevalence of metallo-beta-lactamase-producing acinetobacter baumannii in a teaching hospital in Tabriz, Iran", Jpn J Infect Dis, 64, pp.69~71 (2011)
- Abouelfetouh A, "Phenotypic and genotypic characterization of carbapenemresistant Acinetobacter baumannii isolates from Egypt", Antimicrob Resist Infect Control, 8, pp.185~ (2019)
- Owrang M, "Identification and isolation of insertion sequences, in carbapenem resistant clinical isolates of Acinetobacter baumannii from Tehran hospitals", Jundishapur J Microbiol, 11, pp.e58251~ (2018)
- Wang D, "Characterization of bla(OxA-23)gene regions in isolates of Acinetobacter baumannii", J Microbiol Immunol Infect, 48, pp.284~290 (2015)
- Lee HY, "Wide spread of Tn2006 in an AbaR4-type resistance island among carbapenem-resistant Acinetobacter baumannii clinical isolates in Taiwan", Int J Antimicrob Agents, 40, pp.163~167 (2012)
- Solgi H, "Molecular characterization of intestinal carriage of carbapenem-resistant Enterobacteriaceae among inpatients at two Iranian university hospitals : first report of co-production of bla NDM-7 and bla OXA-48", Eur J Clin Microbiol Infect Dis, 36, pp.2127~2135 (2017)
- Turton JF, "The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii", FEMS Microbiol Lett, 258, pp.72~77 (2006)
- Zhou H, "Dissemination of imipenem-resistant Acinetobacter baumannii strains carrying the ISAba1 blaOXA-23 genes in a Chinese hospital", J Med Microbiol, 56, pp.1076~1080 (2007)
- Merkier AK, "Polyclonal spread of bla(OXA-23)and bla(OXA-58)in Acinetobacter baumannii isolates from Argentina", J Infect Dev Ctries, 2, pp.235~240 (2008)
- Lee MH, "Dissemination of multidrug-resistant Acinetobacter baumannii carrying BlaOxA-23 from hospitals in central Taiwan", J Microbiol Immunol Infect, 46, pp.419~424 (2013)
- Poirel L, "Multiplex PCR for detection of acquired carbapenemase genes", Diagn Microbiol Infect Dis, 70, pp.119~123 (2011)
- Labuschagne Cde J, "Emergence of class 1 integron-associated GES-5 and GES-5-like extended-spectrum beta-lactamases in clinical isolates of Pseudomonas aeruginosa in South Africa", Int J Antimicrob Agents, 31, pp.527~530 (2008)