타이타늄 임플란트 골유착시 성장인자들의 발현 ;TGF-β, IGF-I, BMP2, BMP4
Expression of Growth factors during Osseointegration of Titanium Implant ;TGF-β, IGF-I, BMP2, BMP4
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Osseointegration is a result of bone formation and bone regeneration process, which take place at the interface between bone and implant and biologic determinants such as cytokine, growth factors, bone matrix proteins play an important role in osseointegration. The purpose of this study is to compare the expression of TGF-β, IGF-I, BMP2, BMP4 during osseointegration. We designed an experimental group which was inserted with a RBM surface titanium implants and machined surface, and compared with a control group which had a simple bone cavity and normal bone. Titanium implants were placed into tibia of 8 rabbits. We compared the expression of TGF-β, IGF-I, BMP2, BMP4 using RT-PCR (reverse transcriptase chain reaction) analysis in day 3, 7, 14 and 28 of implant insertion. According to the results, growth factors of experimental groups were more expressed than control groups. Among experimental groups, expression of TGF-β, IGF-I, BMP-4 of RBM group had tendency to increase more at 14th, 28th days than Machined surface group. Therefore, our results suggest that TGF-β, IGF-I, BMP4 are expressed within the bone around the implant and more increased around rough surface implants while osseointegration occurs after dental implant insertion.
Abstract
Osseointegration is a result of bone formation and bone regeneration process, which take place at the interface between bone and implant and biologic determinants such as cytokine, growth factors, bone matrix proteins play an important role in osseointegration. The purpose of this study is to compare the expression of TGF-β, IGF-I, BMP2, BMP4 during osseointegration. We designed an experimental group which was inserted with a RBM surface titanium implants and machined surface, and compared with a control group which had a simple bone cavity and normal bone. Titanium implants were placed into tibia of 8 rabbits. We compared the expression of TGF-β, IGF-I, BMP2, BMP4 using RT-PCR (reverse transcriptase chain reaction) analysis in day 3, 7, 14 and 28 of implant insertion. According to the results, growth factors of experimental groups were more expressed than control groups. Among experimental groups, expression of TGF-β, IGF-I, BMP-4 of RBM group had tendency to increase more at 14th, 28th days than Machined surface group. Therefore, our results suggest that TGF-β, IGF-I, BMP4 are expressed within the bone around the implant and more increased around rough surface implants while osseointegration occurs after dental implant insertion.
참고문헌 (18)
- Albrektsson TO, "Biological aspects of implant dentistry：osseointegration", J Periodontal, 4, pp.58~73 (2000)
- Davies JE, "Understanding peri-implant endosseous healing", J Dent Educ, 67, pp.932~949 (2003)
- Buzza EP, "Effects of electromagnetic field on bone healing around commercially pure titanium surface：histologic and mechanical study in rabbits", Implant Dent, 12, pp.182~187 (2003)
- Clokie ML, "Morphologenic and radioautographic studies of bone formation in relation to titanium implants using the rat tibia as a model", Jomi, 10, pp.1~20 (1995)
- Ysander M, "Intramedullary osseointegration：Development of a rodent model and study of histology and neuropeptide changes around titanium implants", Journal of rehabilitation research and development, 38, pp.183~190 (2001)
- Cooper LF, "Biologic determinants of bone formation for osseointegration： clues for clinical improvements", J Prosthet Dent, 80, pp.439~449 (1998)
- Koka S, "Bone growth factors. Potential for use as an Osseointegration enhancement technique", Periodont Abstr, 43, pp.97~104 (1995)
- Stenport VF, ". FGF-4 and implants：a pilot study in rabbit bone", Clin Oral Impl Res, 14, pp.363~368 (2003)
- Schwartz Z, "Underlying mechanism at the bone - surface interface during regeneration", J Periodont Res, 32, pp.166~171 (1997)
- Fiorellini JP, "Effect on bone healing of bone morphogenetic protein placed in combination with endosseous implants： A pilot study in beagle dogs", Int J Periodontics Restorative Dent, 21, pp.41~47 (2001)
- Ogawa T, "Modulated bone matrix-related gene expression is associated with differences in interfacial strength of different implant surface roughness", Journal of prosthodontics, 11, pp.241~247 (2002)
- Ogawa T, "Different bone integration profiles of turned and acid-etched associated with modulated expression of extracellular matrix genes", Int J Oral Maxillofacial Implants, 18, pp.200~210 (2003)
- Schliephake H, "Bone growth factors in maxillofacial skeletal reconstruction", Int J Oral&Maxillofacial Surgery, 31, pp.469~484 (2002)
- Nakase T, "Transient and Localized Expression of Bone Morphogenetic Protein 4 Messenger RNA During Fracture Healing", Journal of bone and mineral research, pp.651~659 (1994)
- Cochran DL, "Recombinant human bone morphogenetic protein-2 stimulation of bone formation around endosseous dental implants", J Periodontal, 70, pp.139~150 (1999)
- Xiang W, "The effect of bone morphogenetic protein on osseointegration of titnium implants", J Oral Maxillofac Surg, 51, pp.647~651 (1993)
- Clokie ML, "Recombinant human transforming growth factor β-1 and its effects on osseointegration", The journal of craniofacial surgery, 14(3), pp.268~277 (2003)
- Froesch ER, "Action of insulin-like growth factors", Ann Rev Physiol, 47, pp.443~ (1985)