당뇨쥐 상악골에서 즉시 임플란트 후 골유착에 대한 면역조직학적 연구
Immunohistochemical Study of Bone Response to the Immediate Implantation in the Rat Maxilla
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Purpose: Dental implants are used successfully for restoration of dental function. However, the impact of diabetes on the healing of bone associated with immediately placed dental implants is not completely understood. And little is known about the expression of biological molecules involved in bone healing around implants. The purpose of this study was to investigate the bone response around immediately placed titanium implants in the maxilla of ovariectomized rats by immunohistochemical method. Materials and methods: 36 rats were divided into the control, insulin-treated and diabetic groups. Rats received streptozotocin (60 mg/kg) to induce diabetes; animals in the insulin-treated group also received 3 units of subcutaneous slow-release insulin. Two titanium implants (1.2×3 mm) were placed in the extraction socket of maxillary first molars of animals and specimens were harvested at 3 days, 1, 2 and 4 weeks. The expression levels of bone morphogenetic protein (BMP)-4, transforming growth factor (TGF)-β and osteonectin (ON) were measured. Result: The BMP-4 expression increased immediately all groups at day 3, week 1 and week 2. However, staining intensity of diabetic group was weaker than the control and the insulin-treated group but not significant. At week 4, in all groups, the mount of BMP-4 expression decreased. The TGF-β expression instantly showed at day 3 in control group only. At week 1 and 2, TGF-β staining gradually increased in all group. But in the all group staining intensity decreased at 4 week. And we found the expression of ON in the control and the insulin-treated group were higher than that of the diabetic group at 1 weeks and 4 weeks, indicated that bone formation be active in those groups. Conclusion: In this study, insulin treatment increased expression of biologic molecules in the bone around the immediate placed titanium implants in the maxilla of diabetic rat. It is expected that insulin might reverse the reduced predictability of success of immediate implantation with the uncontrolled diabetes patients.
Abstract
Purpose: Dental implants are used successfully for restoration of dental function. However, the impact of diabetes on the healing of bone associated with immediately placed dental implants is not completely understood. And little is known about the expression of biological molecules involved in bone healing around implants. The purpose of this study was to investigate the bone response around immediately placed titanium implants in the maxilla of ovariectomized rats by immunohistochemical method. Materials and methods: 36 rats were divided into the control, insulin-treated and diabetic groups. Rats received streptozotocin (60 mg/kg) to induce diabetes; animals in the insulin-treated group also received 3 units of subcutaneous slow-release insulin. Two titanium implants (1.2×3 mm) were placed in the extraction socket of maxillary first molars of animals and specimens were harvested at 3 days, 1, 2 and 4 weeks. The expression levels of bone morphogenetic protein (BMP)-4, transforming growth factor (TGF)-β and osteonectin (ON) were measured. Result: The BMP-4 expression increased immediately all groups at day 3, week 1 and week 2. However, staining intensity of diabetic group was weaker than the control and the insulin-treated group but not significant. At week 4, in all groups, the mount of BMP-4 expression decreased. The TGF-β expression instantly showed at day 3 in control group only. At week 1 and 2, TGF-β staining gradually increased in all group. But in the all group staining intensity decreased at 4 week. And we found the expression of ON in the control and the insulin-treated group were higher than that of the diabetic group at 1 weeks and 4 weeks, indicated that bone formation be active in those groups. Conclusion: In this study, insulin treatment increased expression of biologic molecules in the bone around the immediate placed titanium implants in the maxilla of diabetic rat. It is expected that insulin might reverse the reduced predictability of success of immediate implantation with the uncontrolled diabetes patients.
참고문헌 (32)
- Lazzara RJ, "Immediate implant placement into extraction sites:surgical and restorative advantages", Int J Periodontics Restorative Dent, 9, pp.332~343 (1989)
- Wagenberg BD, "Immediate implant placement on removal of the natural tooth: retrospective analysis of 1,081 implants", Compend Contin Educ Dent, 22, pp.399~404 (2001)
- Becker W, "Immediate implant placement: treatment planning and surgical steps for successful outcome", Periodontology, 47, pp.79~89 (2008)
- Marder MZ, "Medical conditions affecting the success of dental implants", Compend Contin Educ Dent, 25, pp.739~742 (2004)
- Oczakir C, "Implant-prosthodontic treatment for special care patients: a case series study", Int J Prosthodont, 18, pp.383~389 (2005)
- McMahon MM, "Host defenses and susceptibilityin patients with diabetes mellitus", Infect Dis Clin North Am, 9, pp.1~10 (1995)
- Kotsovilis S, "A comprehensive and critical review of dental implant placement in diabetic animals and patients", Clin Oral Implants Res, 17, pp.587~599 (2006)
- Mombelli A, "Systemic diseases affecting osseointegration therapy", Clin Oral Implants Res, 17, pp.S97~S103 (2006)
- Olson JW, "Dental endosseous implant assessments in a type 2 diabetic population: a prospective study", Int J Oral Maxillofac Implants, 15, pp.811~818 (2000)
- van Steenberghe D, "The relative impact of local and endogenous patient-related factors on implant failure up to the abutment stage", Clin Oral Implants Res, 13, pp.617~622 (2002)
- 표성운, "당뇨쥐에서 임플란트 즉시 식립 후 조직계측학적 연구를 통한 임플란트 골유착 비교", 대한통합치과학회지, 5(2), pp.61~66 (2016), 대한통합치과학회[DOI]
- Fiorellini JP, "The effect of insulin therapy on osseointegration in a diabetic rat model", Clin Oral Implants Res, 10, pp.362~368 (1999)
- McCracken MS, "Bone associated with implants in diabetic and insulin-treated rats", Clin Oral Implants Res, 17, pp.495~500 (2006)
- Takeshita F, "The effects ofdiabetes on the interface between hydroxyapatite implants and bone in rat tibia", J Periodontol, 68, pp.180~185 (1997)
- Siqueira JT, "Bone formation around titanium implants in the rat tibia: role of insulin", Implant Dent, 12, pp.242~251 (2003)
- Hasegawa H, "Type 2 diabetes impairs implant osseointegration capacity in rats", Int J Oral Maxillofac Implants, 23, pp.237~246 (2008)
- Kwon PT, "Maintenance of osseointegration utilizing insulin therapy in a diabetic rat model", J Periodontol, 76, pp.621~626 (2005)
- Suhyun Park, "Biological markers around immediately placed titanium implant in the extraction socket of diabetic and insulin-treated rat maxilla", 대한구강악안면외과학회지, 38(4), pp.204~211 (2012), 대한구강악안면외과학회[DOI]
- Morris HF, "Implant survival in patients with type 2 diabetes: placement to 36 months", Ann Periodontol, 5, pp.157~165 (2000)
- Fiorellini JP, "A retrospective study of dental implants in diabetic patients", Int J Periodontics Restorative Dent, 20, pp.366~373 (2000)
- Goodman WG, "Diminished bone formation in experimental diabetes. Relationship to osteoid maturation and mineralization", Diabetes, 33, pp.825~831 (1984)
- Verhaeghe J, "Bone mineral homeostasis in spontaneously diabetic BB rats. II. Impaired bone turnover and decreased osteocalcin synthesis", Endocrinology, 124, pp.573~582 (1989)
- Reddi AH, "Bone morphogenetic proteins: From basic science to clinical applications", J Bone Joint Surg Am, 83, pp.S63~S69 (2001)
- Katagiri T, "Regulatory mechanisms of osteoblast and osteoclast differentiation", Oral Dis, 8, pp.147~159 (2002)
- Ahrens M, "Expression of human bone morphogenetic proteins-2 or -4 in murine mesenchymal progenitor C3H10T1", 2 cells induces differentiation into distinct mesenchymal cell lineages, 1993, pp.12, 871~880
- Carrington JL, "Accumulation, localization, and compartmentation of transforming growth factor-B during endochondral bone development", J Cell Biol, 107, pp.1969~1975 (1988)
- Bonewald L, "Principles of Bone Biology" (1996), Academic Press
- Rosier RN, "The potential role of transforming growth factor beta in fracture healing", Clin OrthopRelat Res, 355, pp.S294~S300 (1998)
- Schierano G, "Biological factors involved in the osseointegration of oral titanium implants with different surfaces: a pilot study in minipigs", J Periodontol, 76, pp.1710~1720 (2005)
- Boskey AL, "Osteopontin-hydroxyapatite interactions in vitro: inhibition of hydroxy-apatite formation and growth in a gelatin-gel", Bone Miner, 22, pp.147~159 (1993)
- Shirakura M, "Tissue response to titanium implantation in the rat maxilla, with special reference to the effects of surface conditions on bone formation", Clin Oral Implants Res, 14, pp.687~696 (2003)
- Shyng YC, "Bone formation around immediately placed oral implants in diabetic rats", Int J Prosthodont, 19, pp.513~514 (2006)