Fabrication of Biogenic Antimicrobial Silver Nanoparticles by Streptomyces aegyptia NEAE 102 as Eco-Friendly Nanofactory
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The current research was focused on the extracellular biosynthesis of bactericidal silvernanoparticles (AgNPs) using cell-free supernatant of a local isolate previously identified as anovel Streptomyces aegyptia NEAE 102. The biosynthesis of silver nanoparticles by Streptomycesaegyptia NEAE 102 was quite fast and required far less time than previously published strains. The produced particles showed a single surface plasmon resonance peak at 400 nm by UV–Visspectroscopy, which confirmed the presence of AgNPs. Response surface methodology waschosen to evaluate the effects of four process variables (AgNO3 concentration, incubationperiod, pH levels, and inoculum size) on the biosynthesis of silver nanoparticles byStreptomyces aegyptia NEAE 102. Statistical analysis of the results showed that the linear andquadratic effects of incubation period, initial pH, and inoculum size had a significant effect(p < 0.05) on the biosynthesis of silver nanoparticles by Streptomyces aegyptia NEAE 102. Themaximum silver nanoparticles biosynthesis (2.5 OD, at 400 nm ) was achieved in runs number5 and 14 under the conditions of 1 mM AgNO3 (1-1.5% (v/v)), incubation period (72-96 h),initial pH (9-10), and inoculum size (2-4% (v/v)). An overall 4-fold increase in AgNPsbiosynthesis was obtained as compared with that of unoptimized conditions. Thebiosynthesized silver nanoparticles were characterized using UV-VIS spectrophotometer andFourier transform infrared spectroscopy analysis, in addition to antimicrobial properties. Thebiosynthesized AgNPs significantly inhibited the growth of medically important pathogenicgram-positive (Staphylococcus aureus) and gram-negative bacteria (Pseudomonas aeruginosa) andyeast (Candida albicans).
Abstract
The current research was focused on the extracellular biosynthesis of bactericidal silvernanoparticles (AgNPs) using cell-free supernatant of a local isolate previously identified as anovel Streptomyces aegyptia NEAE 102. The biosynthesis of silver nanoparticles by Streptomycesaegyptia NEAE 102 was quite fast and required far less time than previously published strains. The produced particles showed a single surface plasmon resonance peak at 400 nm by UV–Visspectroscopy, which confirmed the presence of AgNPs. Response surface methodology waschosen to evaluate the effects of four process variables (AgNO3 concentration, incubationperiod, pH levels, and inoculum size) on the biosynthesis of silver nanoparticles byStreptomyces aegyptia NEAE 102. Statistical analysis of the results showed that the linear andquadratic effects of incubation period, initial pH, and inoculum size had a significant effect(p < 0.05) on the biosynthesis of silver nanoparticles by Streptomyces aegyptia NEAE 102. Themaximum silver nanoparticles biosynthesis (2.5 OD, at 400 nm ) was achieved in runs number5 and 14 under the conditions of 1 mM AgNO3 (1-1.5% (v/v)), incubation period (72-96 h),initial pH (9-10), and inoculum size (2-4% (v/v)). An overall 4-fold increase in AgNPsbiosynthesis was obtained as compared with that of unoptimized conditions. Thebiosynthesized silver nanoparticles were characterized using UV-VIS spectrophotometer andFourier transform infrared spectroscopy analysis, in addition to antimicrobial properties. Thebiosynthesized AgNPs significantly inhibited the growth of medically important pathogenicgram-positive (Staphylococcus aureus) and gram-negative bacteria (Pseudomonas aeruginosa) andyeast (Candida albicans).
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