Fusarium mangiferae as New Cell Factories for Producing Silver Nanoparticles
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Finding a safe and broad-spectrum medication is a goal of scientists, pharmacists, and physicians, but developing and fabricating the right medicine can be challenging. The current study describes the formation of silver nanoparticles (AgNPs) by Fusarium mangiferae. It involves the antibiofilm activity of the nanoparticles against Staphylococcus aureus. It also involves cytotoxic effect against mammalian cell lines. Well-dispersed nanoparticles are formed by F. mangiferae. The sizes of the nanoparticles were found to range from 25 to 52 nm, and UV-Vis scan showed absorption around 416-420 nm. SEM, TEM, and AFM results displayed spherical and oval shapes. Furthermore, the FTIR histogram detected amide I and amide II compounds responsible for the stability of AgNPs in an aqueous solution. AgNPs were observed to decrease the formation of biofilm at 75% (v/v). DNA reducing, smearing, and perhaps fragmentation were noticed after treating the bacterial cells with 50% (v/v). Additionally, cell lysis was detected releasing proteins in the supernatant. It was also observed that the AgNPs have the ability to cause 59% cervical cancer cell line (HeLa) deaths at 25% (v/v), however, they showed about 31% toxicity against rat embryo fibroblast transformed cell lines (REF). The results of this study prove the efficiency of AgNPs as an antibiofilm against S. aureus, suggesting that AgNPs could be an alternative to antibiotics. It must also be emphasized that AgNPs displayed cytotoxic behavior against mammalian cell lines. Further studies are needed for assessing risk in relation to the possible benefit of prescribing AgNPs.
Abstract
Finding a safe and broad-spectrum medication is a goal of scientists, pharmacists, and physicians, but developing and fabricating the right medicine can be challenging. The current study describes the formation of silver nanoparticles (AgNPs) by Fusarium mangiferae. It involves the antibiofilm activity of the nanoparticles against Staphylococcus aureus. It also involves cytotoxic effect against mammalian cell lines. Well-dispersed nanoparticles are formed by F. mangiferae. The sizes of the nanoparticles were found to range from 25 to 52 nm, and UV-Vis scan showed absorption around 416-420 nm. SEM, TEM, and AFM results displayed spherical and oval shapes. Furthermore, the FTIR histogram detected amide I and amide II compounds responsible for the stability of AgNPs in an aqueous solution. AgNPs were observed to decrease the formation of biofilm at 75% (v/v). DNA reducing, smearing, and perhaps fragmentation were noticed after treating the bacterial cells with 50% (v/v). Additionally, cell lysis was detected releasing proteins in the supernatant. It was also observed that the AgNPs have the ability to cause 59% cervical cancer cell line (HeLa) deaths at 25% (v/v), however, they showed about 31% toxicity against rat embryo fibroblast transformed cell lines (REF). The results of this study prove the efficiency of AgNPs as an antibiofilm against S. aureus, suggesting that AgNPs could be an alternative to antibiotics. It must also be emphasized that AgNPs displayed cytotoxic behavior against mammalian cell lines. Further studies are needed for assessing risk in relation to the possible benefit of prescribing AgNPs.
참고문헌 (50)
- Ahmed AA, "Analyzing formation of silver nanoparticles from the filamentous fungus Fusarium oxysporum and their antimicrobial activity", Turk. J. Biol, 42, pp.54~62 (2018)
- Miller JK, "Nanoparticle deposition onto biofilms", Ann. Biomed. Eng, 41, pp.53~67 (2013)
- Radzig MA, "Antibacterial effects of silver nanoparticles on gram-negative bacteria: influence on the growth and biofilms formation, mechanisms of action", Colloids Surf. B Biointerfaces, 102, pp.300~306 (2013)
- Costerton JW, "Bacterial biofilms : a common cause of persistent infections", Science, 284, pp.1318~1322 (1999)
- Syed MA, "Antibacterial effects of silver nanoparticles on the bacterial strains isolated from catheterized urinary tract infection cases", J. Biomed. Nanotechnol, 5, pp.209~214 (2009)
- Franci G, "Silver nanoparticles as potential antibacterial agents", Molecules, 20, pp.8856~8874 (2015)
- Archer NK, "Staphylococcus aureus biofilms : properties, regulation, and roles in human disease", Virulence, 2, pp.445~459 (2011)
- Alt V, "A n in v itro a ssessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement", Biomaterials, 25, pp.4383~4391 (2004)
- Beyth N, "Alternative antimicrobial approach: nano-antimicrobial materials", J. Evid. Based Complementary Altern. Med, 2015, pp.246012~ (2015)
- Prabhu S, "Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects", Int. Nano. Lett, 2, pp.32~ (2012)
- Hwang IS, "Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved", J. Med. Microbiol, 61, pp.1719~1726 (2012)
- Feng QL, "A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus", J. Biomed. Mater. Res, 52, pp.662~668 (2000)
- Salaheldin TA, "Evaluation of the cytotoxic behavior of fungal extracellular synthesized Ag nanoparticles using confocal laser scanning microscope", Int. J. Mol. Sci, 17, pp.329~ (2016)
- Sriram MI, "Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model", Int. J. Nanomedicine, 5, pp.753~762 (2010)
- Arora S, "Silver nanoparticles protect human keratinocytes against UVB radiation-induced DNA damage and apoptosis : potential for prevention of skin carcinogenesis", Nanomedicine, 11, pp.1265~1275 (2015)
- Stensberg MC, "Toxicological studies on silver nanoparticles : challenges and opportunities in assessment, monitoring and imaging", Nanomedicine, 6, pp.879~898 (2011)
- Hussain SM, "In vitro toxicity of nanoparticles in BRL 3A rat liver cells", Toxicol. In Vitro, 19, pp.975~983 (2005)
- Almofti MR, "Silver ion induces a cyclosporine a-insensitive permeability transition in rat liver mitochondria and release of apoptogenic cytochrome C", J. Biochem, 134, pp.43~49 (2003)
- Carlson C, "Unique cellular interaction of silver nanoparticles: size-dependent generation of reactive oxygen species", J. Phys. Chem. B, 112, pp.13608~13619 (2008)
- Soni N, "Efficacy of fungus mediated silver and gold nanoparticles against Aedes aegypti larvae", Parasitol. Res, 110, pp.175~184 (2012)
- Mishra A, "Bio-synthesis of gold and silver nanoparticles from Candida guilliermondii and their antimicrobial effect against pathogenic bacteria", J. Nanosci. Nanotechnol, 11, pp.243~248 (2011)
- Ahmad A, "Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum", Colloids Surf. B Biointerfaces, 28, pp.313~318 (2003)
- Ingle A, "Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria", Curr. Nanosci, 4, pp.141~144 (2008)
- Dasaratrao Sawle B, "Biosynthesis and stabilization of Au and Au-Ag alloy nanoparticles by fungus, Fusarium semitectum", Sci. Technol. Adv. Mater, 9, pp.035012~ (2008)
- Ingle A, "Fusarium solani:a novel biological agent for the extracellular synthesis of silver nanoparticles", J. Nanopart. Res, 11, pp.2079~2085 (2009)
- Bawaskar M, "A new report on mycosynthesis of silver nanoparticles by Fusarium culmorum", Curr. Nanosci, 6, pp.376~380 (2010)
- Gaikwad SC, "Screening of different Fusarium species to select potential species for the synthesis of silver nanoparticles", J. Braz. Chem. Soc, 24, pp.1974~1982 (2013)
- Gamliel-Atinsky E, "Infection dynamics of Fusarium mangiferae, causal agent of mango malformation disease", Phytopathology, 99, pp.775~781 (2009)
- Omar NH, "Characterization and pathogenicity of Fusarium species associated with leaf spot of mango (Mangifera indica L.)", Microb. Pathog, 114, pp.362~368 (2018)
- Kaiser TD, "Modification of the Congo red agar method to detect biofilm production by Staphylococcus epidermidis", Diagn. Microbiol. Infect. Dis, 75, pp.235~239 (2013)
- Mathur T, "Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods", Indian J. Med. Microbiol, 24, pp.25~29 (2006)
- 김수환, "Antibacterial Activity of Silver-nanoparticles Against Staphylococcus aureus and Escherichia coli", 한국미생물·생명공학회지, 39(1), pp.77~85 (2011), 한국미생물·생명공학회
- Thottappilly G, "Identification and differentiation of isolates of Colletotrichum gloeosporioides from yam by random amplified polymorphic DNA markers", Afr. Crop Sci. J, 7, pp.195~205 (1999)
- Onasanya A, "Genetic fingerprinting and phylogenetic diversity of Staphylococcus aureus isolates from Nigeria", Afr. J. Biotechnol, 2, pp.246~250 (2003)
- Betancur-Galvis LA, "Antitumor and antiviral activity of Colombian medicinal plant extracts", Mem. Inst. Oswaldo Cruz, 94, pp.531~535 (1999)
- Mather JP, "Introduction to Cell and Tissue Culture: Theory and Technique", pp.180~181 (1998), Plenum Press
- Durán N, "Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains", J. Nanobiotech, 3, pp.8~ (2005)
- Gurunathan S, "Multidimensional effects of biologically synthesized silver nanoparticles in Helicobacter pylori, Helicobacter felis, and human lung (L132) and lung carcinoma A549 cells", Nanoscale Res. Lett, 10, pp.35~ (2015)
- Rodríguez-León E, "Synthesis of silver nanoparticles using reducing agents obtained from natural sources (Rumex hymenosepalus extracts)", Nanoscale Res. Lett, 8, pp.318~ (2013)
- Kumar CM, "Biological synthesis of silver nanoparticles from Adansonia digitata L. fruit pulp extract, characterization, and its antimicrobial properties", J. Intercult. Ethnopharmacol, 5, pp.79~85 (2016)
- Gopinath PM, "Mycosynthesis, characterization and antibacterial properties of AgNPs against multidrug resistant (MDR) bacterial pathogens of female infertility cases", Asian J. Pharm. Sci, 10, pp.138~145 (2015)
- Basavaraja SS, "Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum", Mater. Res. Bull, 43, pp.1164~1170 (2008)
- Majeed S, "In vitro study of the antibacterial and anticancer activities of silver nanoparticles synthesized from Penicillium brevicompactum (MTCC-1999)", J. Taibah Univ. Sci, 10, pp.614~620 (2016)
- Chen M, "Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel", Int. J. Nanomedicine, 6, pp.2873~2877 (2011)
- 김성민, "Phase Variation of Biofilm Formation in Staphylococcus aureus by IS 256 Insertion and Its Impact on the Capacity Adhering to Polyurethane Surface", Journal of Korean Medical Science, 19(6), pp.779~782 (2004), 대한의학회
- Milanov D, "Slime production and biofilm forming ability by Staphylococcus aureus bovine mastitis isolates", Acta Vet. Brno, 60, pp.217~226 (2010)
- Abdeen SH, "HeLa-S3 cell growth conditions in serum-free medium and adaptability for proliferation in suspension culture", J. Biol. Sci, 11, pp.124~134 (2011)
- Gillet JP, "The clinical relevance of cancer cell lines", J. J. Natl. Cancer Inst, 105, pp.452~458 (2013)
- Kaba SI, "In vitro studies of the toxic effects of silver nanoparticles on HeLa and U937 cells", Nanotechnol. Sci Appl, 8, pp.19~29 (2015)
- Sambale F, "Investigations of the toxic effect of silver nanoparticles on mammalian cell lines", J. Nanomater, 16, pp.6~ (2015)