본문 바로가기
KERD
논문KCI 등재

Purification and Identification of a Novel Antifungal Protein Secreted by Penicillium citrinum from the Southwest Indian Ocean

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류생물학

초록

A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 culture supernatants with ammonium sulfate and ion-exchange chromatography, an antifungal protein (PcPAF) was purified. The N-terminal amino acid sequence analysis showed that PcPAF might be an unknown antifungal protein. PcPAF displayed antifungal activity against Trichoderma viride, Fusarium oxysporum, Paecilomyces variotii, and Alternaria longipes at minimum inhibitory concentrations of 1.52, 6.08, 3.04, and 6.08 μg/disc, respectively. PcPAF possessed high thermostability and had a certain extent of protease and metal ion resistance. The results suggested that PcPAF may represent a novel antifungal protein with potential application in controlling plant pathogenic fungal infection.

Abstract

A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 culture supernatants with ammonium sulfate and ion-exchange chromatography, an antifungal protein (PcPAF) was purified. The N-terminal amino acid sequence analysis showed that PcPAF might be an unknown antifungal protein. PcPAF displayed antifungal activity against Trichoderma viride, Fusarium oxysporum, Paecilomyces variotii, and Alternaria longipes at minimum inhibitory concentrations of 1.52, 6.08, 3.04, and 6.08 μg/disc, respectively. PcPAF possessed high thermostability and had a certain extent of protease and metal ion resistance. The results suggested that PcPAF may represent a novel antifungal protein with potential application in controlling plant pathogenic fungal infection.

참고문헌 (31)

  1. Bhadury P, "The current status of natural products from marine fungi and their potential as anti-infective agents", J. Ind. Microbiol. Biotechnol, 33, pp.325~337 (2006)
  2. Brown GD, "Hidden killers: human fungal infections", Sci. Trans. Med, 4 (2012)
  3. Chen Z, "Purification and characterization of a novel antifungal protein secreted by Penicillium chrysogenum from an Arctic sediment", Appl. Microbiol. Biotechnol, 97, pp.10381~10390 (2013)
  4. Das S, "Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel", Int. J. Biol. Macromol, 48, pp.38~43 (2011)
  5. Davidson BS., "New dimensions in natural products research: cultured marine microorganisms", Curr. Opin. Biotechnol, 6, pp.284~291 (1995)
  6. Gallo ML, "Antibiotic longchain and α,β-unsaturated aldehydes from the culture of the marine fungus Cladosporium sp", Biochem. Syst. Ecol, 32, pp.545~551 (2004)
  7. Geisen R., "P. nalgiovense carries a gene which is homologus to the paf gene of P. chrysogenum which codes for an antifungal peptide", Int. J. Food Microbiol, 62, pp.95~101 (2000)
  8. Gun Lee D, "Isolation and characterization of a novel antifungal peptide from Aspergillus niger", Biochem. Biophys. Res. Commun, 263, pp.646~651 (1999)
  9. Hegedus N, "Antifungal proteins: more than antimicrobials?", Fungal Biol. Rev, 26, pp.132~145 (2013)
  10. Ivey FD, "Identification of a protective antigen of Coccidioides immitis by expression library immunization", Vaccine, 21, pp.4359~4367 (2003)
  11. Kaiserer L, "Characterization of the Penicillium chrysogenum antifungal protein PAF", Arch. Microbiol, 180, pp.204~210 (2003)
  12. Kim E-K, "Draft genome sequence of Gluconobacter morbifer G707T, a pathogenic gut bacterium isolated from Drosophila melanogaster intestine", J. Bacteriol, 194, pp.1245~ (2012)
  13. Marx F., "Small, basic antifungal proteins secreted from filamentous ascomycetes: a comparative study regarding expression, structure, function and potential application", Appl. Microbiol. Biotechnol, 65, pp.133~142 (2004)
  14. Marx F, "The Penicillium chrysogenum antifungal protein PAF, a promising tool for the development of new antifungal therapies and fungal cell biology studies", Cell. Mol. Life Sci, 65, pp.445~454 (2008)
  15. Meyer V., "A small protein that fights fungi: AFP as a new promising antifungal agent of biotechnological value", Appl. Microbiol. Biotechnol, 78, pp.17~28 (2008)
  16. Moreno AB, "Pathogen-induced production of the antifungal AFP protein from Aspergillus giganteus confers resistance to the blast fungus Magnaporthe grisea in transgenic rice", Mol. Plant Microbe Interact, 18, pp.960~972 (2005)
  17. Muñoz A, "Understanding the mechanism of action of cell-penetrating antifungal peptides using the rationally designed hexapeptide PAF26 as a model", Fungal Biol. Rev, 26, pp.146~155 (2013)
  18. Odell EW., "Peptide antibiotics. Discovery, modes of action and applications", J. Antimicrobial Chemother, 50, pp.149~ (2002)
  19. Olson BH, "Alpha sarcin, a new antitumor agent. I. Isolation, purification, chemical composition, and the identity of a new amino acid", Appl. Microbiol, 13, pp.314~321 (1965)
  20. Osusky M, "Transgenic potatoes expressing a novel cationic peptide are resistant to late blight and pink rot", Transgenic Res, 13, pp.181~190 (2004)
  21. Pennisi E, "The push to pit genomics against fungal pathogens", Science, 292, pp.2273~2274 (2001)
  22. Rodríguez-Martín A, "Characterization of the novel antifungal protein PgAFP and the encoding gene of Penicillium chrysogenum", Peptides, 31, pp.541~547 (2010)
  23. Skouri-Gargouri H, "First isolation of a novel thermostable antifungal peptide secreted by Aspergillus clavatus", Peptides, 29, pp.1871~1877 (2008)
  24. Skouri-Gargouri H, "Factors affecting production and stability of the AcAFP antifungal peptide secreted by Aspergillus clavatus", Appl. Microbiol. Biotechnol, 86, pp.535~543 (2010)
  25. Theis T, "Antifungal proteins: targets, mechanisms and prospective applications", Cell. Mol. Life Sci, 61, pp.437~455 (2004)
  26. Theis T, "The antifungal protein from Aspergillus giganteus causes membrane permeabilization", Antimicrobial Agents Chemother, 47, pp.588~593 (2003)
  27. Uchiya K, "Comparative genome analysis of Mycobacterium avium revealed genetic diversity in strains that cause pulmonary and disseminated disease", PLOS One, 8, pp.71831~ (2013)
  28. Woo JH, "An antifungal protein from the marine bacterium Streptomyces sp. strain AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp", Appl. Environ. Microbiol, 68, pp.2666~2675 (2002)
  29. Wang S, "First report of a novel plant lysozyme with both antifungal and antibacterial activities", Biochem. Biophys. Res. Commun, 327, pp.820~827 (2005)
  30. Wang S, "Isolation of a thermostable legume chitinase and study on the antifungal activity", Appl. Microbiol. Biotechnol, 85, pp.313~321 (2009)
  31. Zasloff M, "Antimicrobial peptides of multicellular organisms", Nature, 415, pp.389~395 (2002)

관련 논문AI추천

이 간행물의 다른 논문 (12건)