본문 바로가기
KERD
논문KCI 등재

Chemical Genetics Approach Reveals Importance of cAMP and MAP Kinase Signaling to Lipid and Carotenoid Biosynthesis in Microalgae

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류생물학

초록

In this study, we attempted to understand signaling pathways behind lipid biosynthesis by employing a chemical genetics approach based on small molecule inhibitors. Specific signaling inhibitors of MAP kinase or modulators of cAMP signaling were selected to evaluate the functional roles of each of the key signaling pathways in three different microalgal species: Chlamydomonas reinhardtii, Chlorella vulgaris, and Haematococcus pluvialis. Our results clearly indicate that cAMP signaling pathways are indeed positively associated with microalgal lipid biosynthesis. In contrast, MAP kinase pathways in three microalgal species are all negatively implicated in both lipid and carotenoid biosynthesis.

Abstract

In this study, we attempted to understand signaling pathways behind lipid biosynthesis by employing a chemical genetics approach based on small molecule inhibitors. Specific signaling inhibitors of MAP kinase or modulators of cAMP signaling were selected to evaluate the functional roles of each of the key signaling pathways in three different microalgal species: Chlamydomonas reinhardtii, Chlorella vulgaris, and Haematococcus pluvialis. Our results clearly indicate that cAMP signaling pathways are indeed positively associated with microalgal lipid biosynthesis. In contrast, MAP kinase pathways in three microalgal species are all negatively implicated in both lipid and carotenoid biosynthesis.

참고문헌 (24)

  1. Alaimo PJ, "Chemical genetic approaches for the elucidation of signaling pathways", Curr. Opin. Chem. Biol, 5, pp.360~367 (2001)
  2. Blaby IK, "Systems-level analysis of nitrogen starvation-induced modifications of carbon metabolism in a Chlamydomonas reinhardtii starchless mutant", Plant Cell, 25, pp.4305~4323 (2013)
  3. Choi Y-E, "MVE1, a velvet gene in Mycosphaerella graminicola is associated with melanin biosynthesis, and dimorphic change, and pathogenicity", Appl. Environ. Microbiol, 77, pp.942~953 (2011)
  4. Choi Y-E, "Functional characterization of Fusarium verticillioides CPP1, a gene encoding putative protein phosphatase 2A catalytic subunit", Microbiology, 154, pp.326~336 (2008)
  5. Choi Y-E, "The cAMP signaling pathway in Fusarium verticillioides is important for conidiation, plant infection, and stress responses but not fumonisin production", Mol. Plant Microbe Interact, 23, pp.522~533 (2010)
  6. Choi Y-E, "Evaluation of factors promoting the astaxanthin production by a unicellular green alga, Haematococcus pluvialis, with fractional factorial design", Biotechnol. Prog, 18, pp.1170~1175 (2002)
  7. Cooksey KE, "Fluorometic determination of the neutral lipid content of microalgal cells using Nile Red", J. Microbiol. Methods, 6, pp.333~345 (1987)
  8. Davies BH, "Chemistry and Biochemistry of Plant Pigments, Vol. 2", pp.38~155 (1976), Academic Press
  9. Demirbas A, "Oily products from mosses and algae via pyrolysis", Energ. Source. A, 28, pp.933~940 (2006)
  10. D’Souza C, "Conserved cAMP signaling cascades regulate fungal development and virulence", FEMS Microbiol. Rev, 25, pp.349~364 (2001)
  11. Folch J, "A simple method for the isolation and purification of total lipides from animal tissues", J. Biol. Chem, 226, pp.497~509 (1957)
  12. Goodenough UW, "Cyclic AMP enhances the sexual agglutinability of Chlamydomonas flagella", J. Cell Biol, 109, pp.247~252 (1989)
  13. Goodenough U, "The path to triacylglyceride obesity in the sta6 strain of Chlamydomonas reinhardtii", Eukaryot. Cell, 13, pp.591~613 (2014)
  14. Gustin MC, "Map kinase pathways in the yeast Saccharomyces cerevisiae", Microbiol. Mol. Biol. Rev, 62, pp.1264~1300 (1998)
  15. Lengeler KB, "Signal transduction cascades regulating fungal development and virulence", Microbiol. Mol. Biol. Rev, 64, pp.746~785 (2000)
  16. Lichtenthaler HK, "Chlorophylls and carotenoids:pigments of photosynthetic biomembranes", Methods Enzymol, 148, pp.350~382 (1987)
  17. Lorenz RT, "Commercial potential for Haematococcus microalgae as a natural source of astaxanthin", Trends Biotechnol, 18, pp.160~167 (2000)
  18. Meskiene I, "MAP kinase pathways: molecular plug-and-play chips for the cell", Plant Mol. Biol, 42, pp.791~806 (2000)
  19. Nakamura DN, "Journally speaking: the mass appeal of biomass", Oil Gas J, 104, pp.15~ (2006)
  20. Park S-J, "Serial optimization of biomass production using microalga Nannochloris oculata and corresponding lipid biosynthesis", Bioprocess Biosyst. Eng, 35, pp.3~9 (2011)
  21. Seely GR, "Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide", Mar. Biol, 12, pp.184~188 (1972)
  22. Skamnioti P, "Magnaporthe grisea cutinase2mediates appressorium differentiation and host penetration and is required for full virulence", Plant Cell, 19, pp.2674~2689 (2007)
  23. Stockwell BR, "Chemical genetics: ligand-based discovery of gene function", Nat. Rev. Genet, 1, pp.116~125 (2000)
  24. Wase N, "Integrated quantitative analysis of nitrogen stress response in Chlamydomonas reinhardtii using metabolite and protein profiling", J. Proteome Res, 13, pp.1373~1396 (2014)

관련 논문AI추천

Increasing the Triacylglycerol Content in Dunaliella tertiolecta through Isolation of Starch-Deficient MutantsAnchalee Sirikhachornkit, Anongpat Suttangkakul, Kittisak Yokthongwattana, Kunn Kangvansaichol, Metha Meetam, Piyada Juntawong, Prayad Pokethitiyook, Supachai Vuttipongchaikij한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제5호Biotechnological Approaches for Biomass and Lipid Production Using Microalgae Chlorella and Its Future PerspectivesJe Sujeong, YASUYO YAMAOKA한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2022 · 제32권 제11호CGI-58 Protein Acts as a Positive Regulator of Triacylglycerol Accumulation in Phaeodactylum tricornutumHu Hanhua, Pan Yufang, Shu Qin한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2023 · 제33권 제2호Isolation and Proteomic Analysis of a Chlamydomonas reinhardtii Mutant with Enhanced Lipid Production by the Gamma Irradiation Method박현, 임상용, Jaewon Baek, Jong-Il Choi, Si Jae Park한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제12호Enhanced Production of Fatty Acids via Redirection of Carbon Flux in Marine Microalga Tetraselmis sp.이철균, 이후근, 조병관, 최형균, 한미애, 홍성주, Z-Hun Kim한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제2호Overexpression of S-Adenosylmethionine Synthetase in Recombinant Chlamydomonas for Enhanced Lipid Production최종일, Ahn Joon Woo, Kim Jeong Hyeon, Park Eun-Jeong한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2023 · 제33권 제3호

이 간행물의 다른 논문 (14건)