Isolation and Proteomic Analysis of a Chlamydomonas reinhardtii Mutant with Enhanced Lipid Production by the Gamma Irradiation Method
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In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.
Abstract
In this study, an enhanced lipid-producing mutant strain of the microalga Chlamydomonas reinhardtii was developed by gamma irradiation. To induce the mutation, C. reinhardtii was gamma irradiated at a dose of 400 Gy. After irradiation, the surviving cells were stained with Nile red. The mutant (Cr-4013) accumulating 20% more lipid than the wild type was selected. Thin-layer chromatography revealed the triglyceride and free fatty acid contents to be markedly increased in Cr-4013. The major fatty acids identified were palmitic acid, oleic acid, linoleic acid, and linolenic acid. Random amplified polymeric DNA analysis showed partial genetic modifications in Cr-4013. To ascertain the changes of protein expression in the mutant strain, two-dimensional electrophoresis was conducted. These results showed that gamma radiation could be used for the development of efficient microalgal strains for lipid production.
참고문헌 (40)
- Ahloowalia BS, "Induced mutations –a new paradigm in plant breeding", Euphytica, 118, pp.167~173 (2001)
- Ahmad AL, "Microalgae as a sustainable energy source for biodiesel production: a review", Renew. Sustain. Energy Rev, 15, pp.584~593 (2011)
- Amaro HM, "Advances and perspectives in using microalgae to produce biodiesel", Appl. Energy, 88, pp.3402~3410 (2011)
- Beer LL, "Engineering algae for biohydrogen and biofuel production", Curr. Opin. Biotechnol, 20, pp.264~271 (2009)
- Berberoglu H, "Radiation characteristics of Botryococcus braunii, Chlorococcum littorale, and Chlorella sp. used for CO2 fixation and biofuel production", J. Quant. Spectrosc. Radiat. Transf, 110, pp.1879~1893 (2009)
- Bligh EG, "A rapid method of total lipid extraction and purification", Can. J. Biochem. Physiol, 37, pp.911~917 (1959)
- Brennan L, "Biofuels from microalgae - a review of technologies for production, processing, and extractions of biofuels and co-products", Renew. Sustain. Energy Rev, 14, pp.557~577 (2010)
- Browse J, "Fatty acid composition of leaf lipids determined after combined digestion and fatty acid methyl ester formation from freshtissue", Anal. Biochem, 152, pp.141~145 (1986)
- Brune DE, "Microalgal biomass for greenhouse gas reductions: potential for replacement of fossil fuels and animal feeds", J. Environ. Eng, 135, pp.1136~1144 (2009)
- Chen W, "A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae", J. Microbiol. Methods, 77, pp.41~47 (2009)
- Demirbas A, "Importance of biodiesel as transportation fuel", Energy Policy, 35, pp.4661~4670 (2007)
- Dismukes GC, "Aquatic phototrophs: efficient alternatives to landbased crops for biofuels", Curr. Opin. Biotechnol, 19, pp.235~240 (2008)
- Duffy JE, "Biofuels:algae", Science, 326, pp.1345~ (2009)
- Ghirardi ML, "Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms", Annu. Rev. Plant Biol, 58, pp.71~91 (2007)
- Gillet S, "Cadmium response and redoxin targets in Chlamydomonas reinhardtii: a proteomic approach", Photosynth. Res, 89(2-3), pp.201~211 (2006)
- Goodenough UW, "Green yeast", Cell, 70, pp.533~538 (1992)
- Hankamer B, "The solar bio-fuels consortium: developing advanced biofuel production systems", Photosynth. Res, 91, pp.136~136 (2007)
- Horling F, "Divergent light-, ascorbate-, and oxidative stress-dependent regulation of expression of the peroxiredoxin gene family in Arabidopsis", Plant Physiol, 131, pp.317~325 (2003)
- Hu Q, "Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances", Plant J, 54, pp.621~639 (2008)
- Illman AM, "Increase in Chlorella strains calorific values when grown in low nitrogen medium", Enzyme Microb. Technol, 27, pp.631~635 (2000)
- 강시온, "A Cost Analysis of Microalgal Biomass and Biodiesel Production in Open Raceways Treating Municipal Wastewater and under Optimum Light Wavelength", Journal of Microbiology and Biotechnology, 25(1), pp.109~118 (2015), 한국미생물·생명공학회[DOI]
- Kromina KA, "Role of peptidyl-prolyl-cis", trans-isomerases in pathologic processes, 2008, pp.2, 195~202
- Kurisu G, "Structure of the cytochrome b6f complex of oxygenic photosynthesis : tuning the cavity", Science, 302, pp.1009~1014 (2003)
- Lepage G, "Improved recovery of fatty acid through direct transesterification without prior extraction or purification", J. Lipid Res, 25, pp.1391~1396 (1984)
- Mayfield SP, "Expression of the nuclear gene encoding oxygen-evolving enhancer protein 2 is required for high levels of photosynthetic oxygen evolution in Chlamydomonas reinhardtii", Proc. Natl. Acad. Sci. USA, 84, pp.749~753 (1987)
- McGinnis KM, "Characterization of the growth and lipid content of the diatom Chaetoceros muelleri", J. Appl. Phycol, 9, pp.19~24 (1997)
- Mofijur M, "Comparative evaluation of performance and emission characteristics of Moringa oleifera and palm oil based biodiesel in a diesel engine", Ind. Crops Prod, 53, pp.78~84 (2014)
- Ni Z, "Altered circadian rhythms regulate growth vigour in hybrids and allopolyploids", Nature, 457, pp.327~331 (2008)
- Peltier JB, "Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction", Plant Cell Online, 14, pp.211~236 (2002)
- Pienkos PT, "The promise and challenges of microalgal-derived biofuels", Biofuels Bioproducts Biorefining, 3, pp.431~440 (2009)
- Sato N, "Dual role of methionine in the biosynthesis of diacylglyceryl-trimethylhomoserine in Chlamydomonas reinhardtii", Plant Physiol, 86, pp.931~934 (1998)
- Scurlock JMO, "Utilising biomass crops as an energy source: a European perspective", Water Air Soil Pollut, 70, pp.499~518 (1993)
- Siaut M, "Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves", BMC Biotechnol, 11, pp.7~ (2011)
- Suda Y, "Isolation and characterization of a novel antistress gene from Chlamydomonas sp. W80", J. Biosci. Bioeng, 107, pp.352~354 (2009)
- Tucker WC, "Role of the ATP synthase α-subunit in conferring sensitivity to tentoxin", Biochemistry, 40, pp.7542~7548 (2001)
- Wang L, "Proteomic analysis of leaves from a diploid cybrid produced by protoplast fusion between Satsuma mandarin and pummelo", Plant Cell Tissue Organ Cult, 103, pp.165~174 (2010)
- Work VH, "Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complementedstrains. Eukaryot", Cell, 9, pp.1251~1261 (2010)
- Lei Wu, "Screening and Characterization of Oleaginous Microalgal Species from Northern Xinjiang", Journal of Microbiology and Biotechnology, 25(6), pp.910~917 (2015), 한국미생물·생명공학회[DOI]
- Yoon M, "Proteomic analysis of Spirogyra varians mutant with high starch content and growth rate induced by gamma irradiation", Bioprocess Biosyst. Eng, 36, pp.765~774 (2013)
- Yanlin Zheng, "Lipid Production by a CO2-Tolerant Green Microalga, Chlorella sp. MRA-1", Journal of Microbiology and Biotechnology, 24(5), pp.683~689 (2014), 한국미생물·생명공학회[DOI]
