본문 바로가기
KERD
논문KCI 등재

Biodistribution of a Promising Probiotic, Bifidobacterium longum subsp. longum Strain BBMN68, in the Rat Gut

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류생물학

초록

Bifidobacterium longum subsp. longum BBMN68, isolated from centenarians in Guangxi, China, has been proved to be a promising probiotic strain for its health benefits. In this study, the biodistribution of this strain in the rat gut was first investigated using the quantitative realtime PCR assay and propidium monoazide. Strain-specific primers were originally designed based on the BBMN68 genome sequence. Healthy rats were orally inoculated with either a single dose of BBMN68 (1010 colony-forming units/kg), or with one dose per day for 7 days and bacterial concentrations were analyzed in detail from the intestinal contents and feces of four different gut locations, including stomach, small intestine, colon, and rectum. Results indicated that strain BBMN68 could overcome the rigors of passage through the upper gastrointestinal tract and transiently accumulate in the colon, even though survival in the stomach and small intestine was not high. A good level of BBMN8 could stay in vivo for 72 h following a 7-day oral administration, and a daily administration is suggested for a considerable and continuous population of BBMN68 to be maintained in the host intestine.

Abstract

Bifidobacterium longum subsp. longum BBMN68, isolated from centenarians in Guangxi, China, has been proved to be a promising probiotic strain for its health benefits. In this study, the biodistribution of this strain in the rat gut was first investigated using the quantitative realtime PCR assay and propidium monoazide. Strain-specific primers were originally designed based on the BBMN68 genome sequence. Healthy rats were orally inoculated with either a single dose of BBMN68 (1010 colony-forming units/kg), or with one dose per day for 7 days and bacterial concentrations were analyzed in detail from the intestinal contents and feces of four different gut locations, including stomach, small intestine, colon, and rectum. Results indicated that strain BBMN68 could overcome the rigors of passage through the upper gastrointestinal tract and transiently accumulate in the colon, even though survival in the stomach and small intestine was not high. A good level of BBMN8 could stay in vivo for 72 h following a 7-day oral administration, and a daily administration is suggested for a considerable and continuous population of BBMN68 to be maintained in the host intestine.

참고문헌 (42)

  1. Ahlroos T, "Quantitative strain-specific detection of Lactobacillus rhamnosus GG in human faecal samples by real-time PCR", J. Appl. Microbiol, 106, pp.506~514 (2009)
  2. Annan N, "Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastro-intestinal conditions", Food Res. Int, 41, pp.184~193 (2008)
  3. Arakawa A, "Reduction of Clostridium perfringens by feed additive antibiotics in ceca of chickens infected with Eimeria tenella", Poult. Sci, 54, pp.1000~1007 (1975)
  4. Charteris W, "Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract", J. Appl. Microbiol, 84, pp.759~768 (1998)
  5. Conway PL, "Survival of lactic-acid bacteria in the human stomach and adhesion to intestinal-cells", J. Dairy Sci, 70, pp.1~12 (1987)
  6. Davis C, "Enumeration of probiotic strains: review of culture-dependent and alternative techniques to quantify viable bacteria", J. Microbiol. Methods, 103, pp.9~17 (2014)
  7. Fujimoto J, "Identification and quantification of Lactobacillus casei strain Shirota in human feces with strain-specific primers derived from randomly amplified polymorphic DNA", Int. J. Food Microbiol, 126, pp.210~215 (2008)
  8. Fujimoto J, "Identification and quantification of viable Bifidobacterium breve strain Yakult in human faeces by using strain-specific primers and propidium monoazide", J. Appl. Microbiol, 110, pp.209~217 (2011)
  9. Fujimoto J, "Quantitative detection of viable Bifidobacterium bifidum BF-1 cells in human feces by using propidium monoazide and strain-specific primers", Appl. Environ. Microbiol, 79, pp.2182~2188 (2013)
  10. Gilliland SE, "Health and nutritional benefits from lactic acid bacteria", FEMS Microbiol. Lett, 87, pp.175~188 (1990)
  11. Gonzalez-Rodriguez I, "Factors involved in the colonization and survival of bifidobacteria in the gastrointestinal tract", FEMS Microbiol. Lett, 340, pp.1~10 (2013)
  12. Hao Y, "Complete genome sequence of Bifidobacterium longum subsp. longum BBMN68, a new strain from a healthy Chinese centenarian", J. Bacteriol, 193, pp.787~788 (2011)
  13. Izquierdo E, "Resistance to simulated gastrointestinal conditions and adhesion to mucus as probiotic criteria for Bifidobacterium longum strains", Curr. Microbiol, 56, pp.613~618 (2008)
  14. Karjalainen H, "Real-time PCR assays for strain-specific quantification of probiotic strains in human faecal samples", Int. Dairy J, 27, pp.58~64 (2012)
  15. Kimoto H, "Survival of lactococci during passage through mouse digestive tract", Can. J. Microbiol, 49, pp.707~711 (2003)
  16. Marteau P, "Survival of lactic acid bacteria in a dynamic model of the stomach and small intestine: validation and the effects of bile", J. Dairy Sci, 80, pp.1031~1037 (1997)
  17. Marteau P, "Potential of using lactic acid bacteria for therapy and immunomodulation in man", FEMS Microbiol. Rev, 12, pp.207~220 (1993)
  18. Mathys S, "PCR and realtime PCR primers developed for detection and identification of Bifidobacterium thermophilum in faeces", BMC Microbiol, 8, pp.179~ (2008)
  19. Matsuki T, "Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria", Appl. Environ. Microbiol, 70, pp.167~173 (2004)
  20. Matsuki T, "Genus- and species-specific PCR primers for the detection and identification of bifidobacteria", Curr. Issues Intest. Microbiol, 4, pp.61~69 (2003)
  21. Maus JE, "Employment of stressful conditions during culture production to enhance subsequent cold- and acid-tolerance of bifidobacteria", J. Appl. Microbiol, 95, pp.146~154 (2003)
  22. Nocker A, "Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells", J. Microbiol. Methods, 67, pp.310~320 (2006)
  23. Nocker A, "Use of propidium monoazide for live", dead distinction in microbial ecology, 2007, pp.73, 5111~5117
  24. Parvez S, "Probiotics and their fermented food products are beneficial for health", J. Appl. Microbiol, 100, pp.1171~1185 (2006)
  25. Rowland IR, "Effect of Bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats", Carcinogenesis, 19, pp.281~285 (1998)
  26. Roy D., "Media for the isolation and enumeration of bifidobacteria in dairy products", Int. J. Food Microbiol, 69, pp.167~182 (2001)
  27. Sánchez B, "Low-pH adaptation and the acid tolerance response of Bifidobacterium longum biotype longum", Appl. Environ. Microbiol, 73, pp.6450~6459 (2007)
  28. Sattler VA, "Development of a strain-specific real-time PCR assay for enumeration of a probiotic Lactobacillus reuteri in chicken feed and intestine", PLoS One, 9, pp.e90208~ (2014)
  29. Schell D, "Fluidized bed microencapsulation of Lactobacillus reuteri with sweet whey and shellac for improved acid resistance and in-vitro gastro-intestinal survival", Food Res. Int, 62, pp.308~314 (2014)
  30. Senok AC, "Probiotics: facts and myths", Clin. Microbiol. Infect, 11, pp.958~966 (2005)
  31. Solieri L, "Development of a sequencecharacterized amplified region marker-targeted quantitative PCR assay for strain-specific detection of Oenococcus oeni during wine malolactic fermentation", Appl. Environ. Microbiol, 76, pp.7765~7774 (2010)
  32. Su P, "Prebiotics enhance survival and prolong the retention period of specific probiotic inocula in an in vivo murine model", J. Appl. Microbiol, 103, pp.2392~2400 (2007)
  33. Taverniti V, "Short-term daily intake of 6 billion live probiotic cells can be insufficient in healthy adults to modulate the intestinal bifidobacteria and lactobacilli", J. Funct. Foods, 6, pp.482~491 (2014)
  34. Toshimitsu T, "Strain-specific identification of Bifidobacterium bifidum OLB6378 by PCR", Biosci. Biotechnol. Biochem, 77, pp.572~576 (2013)
  35. Tripathi MK, "Probiotic functional foods:survival of probiotics during processing and storage", J. Funct. Foods, 9, pp.225~241 (2014)
  36. Turnbaugh PJ, "The human microbiome project: exploring the microbial part of ourselves in a changing world", Nature, 449, pp.804~ (2007)
  37. Turroni F, "Exploring the diversity of the bifidobacterial population in the human intestinal tract", Appl. Environ. Microbiol, 75, pp.1534~1545 (2009)
  38. Wells CL, "Role of intestinal anaerobic-bacteria in colonization resistance", Eur. J. Clin. Microbiol. Infect. Dis, 7, pp.107~113 (1988)
  39. Xiao M, "Oxidative stress-related responses of Bifidobacterium longum subsp. longum BBMN68 at the proteomic level after exposure to oxygen", Microbiology, 157, pp.1573~1588 (2011)
  40. Yang H, "Oral administration of live Bifidobacterium substrains isolated from centenarians enhances intestinal function in mice", Curr. Microbiol, 59, pp.439~445 (2009)
  41. Yang HY, "Oral administration of live Bifidobacterium substrains isolated from healthy centenarians enhanced immune function in BALB", c mice, 2009, pp.29, 281~289
  42. Zhu J, "Immunomodulatory effects of novel Bifidobacterium and Lactobacillus strains on murine macrophage cells", Afr. J. Microbiol Res, 5, pp.8~15 (2011)

관련 논문AI추천

Development of Strain-Specific Primers for Identification of Bifidobacterium bifidum BGN4박명수, 윤소연, 지근억, 한유리한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2017 · 제27권 제5호Isolation and Characterization of Bifidobacterium longum subsp. longum BCBR-583 for Probiotic Applications in Fermented Foods김유태, 김철홍, 신영섭, 이다혜, 이주훈한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제11호The Differences between Luminal Microbiota and Mucosal Microbiota in MiceGenshen Zhong, Jianmin Li, Mingyong Wang, Minna Wu, Puze Li, Yunying An한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2020 · 제30권 제2호Effect of Lactobacillus rhamnosus hsryfm 1301 on the Gut Microbiota and Lipid Metabolism in Rats Fed a High-Fat DietBoxing Yin, Dawei Chen, Feixiang Guo, Haiqing Zhao, Jiadi Huang, Ruixia Gu, Xia Chen, Yujun Huang, Yun Wu, Zhenquan Yang한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2015 · 제25권 제5호Gut Microbiota Community and Its Assembly Associated with Age and Diet in Chinese CentenariansDa Cai, Danlei Li, Fang Wang, Guohong Huang, Haiyan Su, Lian Yu, Mouming Zhao, Peihong Shen, Quanyang Li, Ruifeng Mao, Ting Yu, Xiaolin Liang, Yang Yang, Zhenjun Zhu한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2015 · 제25권 제8호The Functional Roles of Lactobacillus acidophilus in Different Physiological and Pathological ProcessesChen Xiatian, Gao Huijuan, Hai Deng, Li Peifeng, Li Xin, Wei Chuang, Zhang Lei한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2022 · 제32권 제10호

이 간행물의 다른 논문 (13건)