Hydrogel Dressing with a Nano-Formula against Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa Diabetic Foot Bacteria
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This study proposes an alternative approach for the use of chitosan silver-based dressing for the control of foot infection with multidrug-resistant bacteria. Sixty-five bacterial isolates were isolated from 40 diabetic patients. Staphylococcus aureus (37%) and Pseudomonas aeruginosa (18.5%) were the predominant isolates in the ulcer samples. Ten antibiotics were in vitro tested against diabetic foot clinical bacterial isolates. The most resistant S. aureus and P. aeruginosa isolates were then selected for further study. Three chitosan sources were tested individually for chelating silver nanoparticles. Squilla chitosan silver nanoparticles (Sq. Cs-Ag0) showed the maximum activity against the resistant bacteria when mixed with amikacin that showed the maximum synergetic index. This, in turn, resulted in the reduction of the amikacin MIC value by 95%. For evaluation of the effectiveness of the prepared dressing using Artemia salina as the toxicity biomarker, the LC50 was found to be 549.5, 18,000, and 10,000 μg/ml for amikacin, Sq. Cs-Ag0, and dressing matrix, respectively. Loading the formula onto chitosan hydrogel dressing showed promising antibacterial activities, with responsive healing properties for the wounds in normal rats of those diabetic rats (polymicrobial infection). It is quite interesting to note that no emergence of any side effect on either kidney or liver biomedical functions was noticed.
Abstract
This study proposes an alternative approach for the use of chitosan silver-based dressing for the control of foot infection with multidrug-resistant bacteria. Sixty-five bacterial isolates were isolated from 40 diabetic patients. Staphylococcus aureus (37%) and Pseudomonas aeruginosa (18.5%) were the predominant isolates in the ulcer samples. Ten antibiotics were in vitro tested against diabetic foot clinical bacterial isolates. The most resistant S. aureus and P. aeruginosa isolates were then selected for further study. Three chitosan sources were tested individually for chelating silver nanoparticles. Squilla chitosan silver nanoparticles (Sq. Cs-Ag0) showed the maximum activity against the resistant bacteria when mixed with amikacin that showed the maximum synergetic index. This, in turn, resulted in the reduction of the amikacin MIC value by 95%. For evaluation of the effectiveness of the prepared dressing using Artemia salina as the toxicity biomarker, the LC50 was found to be 549.5, 18,000, and 10,000 μg/ml for amikacin, Sq. Cs-Ag0, and dressing matrix, respectively. Loading the formula onto chitosan hydrogel dressing showed promising antibacterial activities, with responsive healing properties for the wounds in normal rats of those diabetic rats (polymicrobial infection). It is quite interesting to note that no emergence of any side effect on either kidney or liver biomedical functions was noticed.
참고문헌 (61)
- Andrews JM, "Determination of minimum inhibitory concentrations", J. Antimicrob. Chemother, 48, pp.5~16 (2001)
- Anisha BS, "Chitosan–hyaluronic acid", nano silver composite sponges for drug resistant bacteria infected diabetic wounds, 2013, pp.62, 310~320
- Anuj SN, "Identification of Pseudomonas aeruginosa by a duplex real-time polymerase chain reaction assay targeting the ecfX and the gyrB genes", Diagn. Microbiol. Infect. Dis, 63, pp.127~131 (2009)
- Archana D, "Evaluation of chitosan nano dressing for wound healing: characterization, in vitro and in vivo studies", Int. J. Biol. Macromol, 57, pp.193~203 (2013)
- Baran ET, "Starch-chitosan hydrogels prepared by reductive alkylation cross-linking", J. Mater. Sci. Med, 15, pp.759~765 (2004)
- Berg JO, "Antibacterial properties of EMLAs and lidocaine in wound tissue biopsies for culturing", Wound Repair Regen, 14, pp.581~585 (2006)
- Clinical and Laboratory Standards Institute, "Performance standards for antimicrobial susceptibility testing. Twenty-Second Informational Supplement Document M100-S22", CLSI, 31, pp.62~86 (2012)
- De Carvalho EN, "Experimental model of induction of diabetes mellitus in rats", Acta Cir. Bras, 18, pp.60~64 (2003)
- De Vos D, "Detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL", J. Clin. Microbiol, 35, pp.1295~1299 (1997)
- Dhillon GS, "Recent development in applications of important biopolymer chitosan in biomedicine, pharmaceuticals and personal care products", Curr. Tiss. Eng, 2, pp.20~40 (2013)
- El-Naggar MYM, "Synergistic effect of actinomycin X2produced by Streptomyces nasri strain YG62 with other antibiotics", Biomed. Lett, 58, pp.169~173 (1998)
- Gardner SE, "The validity of the clinical signs and symptoms used to identify localized chronic wound infection", Wound Repair Regen, 9, pp.178~186 (2001)
- Geha DJ, "Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory", J. Clin. Microbiol, 32, pp.1768~1772 (1994)
- Gohar YM, "Marine chitosan-silver nano-particles as antibiotic synergizers against diabetic foot bacteria", Arch. Clin. Microbiol, 4, pp.1~7 (2013)
- Grimble SA, "Methicillin resistant Staphylococcus aureus in patients undergoing major amputation", Eur. J. Vasc. Endovasc. Surg, 22, pp.215~218 (2001)
- Harikrishnan R, "Dietary supplementation with chitin and chitosan on hematology and innate immune response in Epinephelus bruneus against Philasterides dicentrarchi", Exp. Parasitol, 131, pp.116~124 (2012)
- Himly N, "Poly (Nvinylpyrrolidone)hydrogels: hydrogel composites as wound dressing for tropical environment", Radiat. Phys. Chem, 42, pp.911~914 (1993)
- Hussien IA, "Identification of Pseudomonas aeruginosa from burn wounds isolates by using PCR exatoxin-A specific primers", Iraqi J. Biotechnol, 11, pp.282~291 (2012)
- Jonas D, "Evaluation of the mecA and femB duplex polymerase chain reaction for detection of methicillinresistant Staphylococcus aureus", Eur. J. Clin. Microbiol. Infect. Dis, 18, pp.643~647 (1999)
- Jude EB, "Prospective randomized controlled study of Hydrofiber® dressing containing ionic silver or calcium alginate dressings in nonischemic diabetic foot ulcers", Diabet. Med, 24, pp.280~288 (2007)
- Keith CT, "Multicomponent therapeutics for networked systems", Nat. Rev. Drug Discov, 4, pp.71~78 (2005)
- Khan AA, "Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR", Appl. Environ. Microbiol, 60, pp.3739~3745 (1994)
- Kobayashi N, "Detection of mecA, femA, and femB genes in clinical strains of staphylococci using polymerase chain reaction", Epidemiol. Infect, 113, pp.259~266 (1994)
- Lee DW, "Advances in chitosan material and its hybrid derivatives: a review", Open Biomater. J, 1, pp.10~20 (2009)
- Lee YH, "Acceleration of wound healing in diabetic rats by layered hydrogel dressing", Carbhydr. Polym, 88, pp.809~819 (2012)
- Lin T, "Development of a duplex realtime PCR method for the pharmaceutical rapid microbial detection of Staphylococcus aureus and Pseudomonas aeruginosa", J. Biosci. Med, 2, pp.12~19 (2014)
- Lodise TP, "Larger vancomycin doses (at least 4 grams per day) are associated with an increased incidence of nephrotoxicity", Antimicrob. Agents Chemother, 52, pp.1330~1336 (2008)
- Maguire GP, "Clinical experience and outcomes of community acquired and nosocomial methicillin-resistant Staphylococcus aureus in a northern Australian hospital", J. Hosp. Infect, 38, pp.273~281 (1998)
- Masson-Meyers DS, "Antimicrobial effects of Copaifera langsdorffii oleoresin infected rat wounds", Int. J. Appl. Microbiol. Sci, 2, pp.9~20 (2013)
- Mayer BN, "Brine shrimp: a convenient general bioassay for active plant constituents", Planta Med, 45, pp.31~34 (1982)
- Mesaros N, "Pseudomonas aeruginosa: resistance and therapeutic options at the turn of the new millennium", Clin. Microbiol. Infect, 13, pp.560~578 (2007)
- Mizuno K, "Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice", J. Biomed. Mater. Res, 64, pp.177~181 (2002)
- Mock JJ, "Shape effects in plasmon resonance of individual colloidal silver nanoparticles", J. Chem. Phys, 116, pp.6755~6760 (2002)
- Mohammed FA, "Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria", J. Nanomed, 6, pp.103~109 (2010)
- Motoshima M, "Rapid and accurate detection of Pseudomonas aeruginosa by real-time polymerase chain reaction with melting curve analysis targeting gyrB gene", Diagn. Microbiol. Infect. Dis, 58, pp.53~58 (2007)
- Moura LIF, "Recent advances on the development of wound dressings for diabetic foot ulcer treatment", Acta Biomater, 9, pp.7093~7114 (2013)
- Nagai G, "Entry of aminoglycosides into renal tubular epithelial cells via endocytosis-dependent and endocytosis-independent pathways", Biochem. Pharmacol, 90, pp.331~337 (2014)
- O’Loughlin A, "Topical stem and progenitor cell therapy for diabetic foot ulcers", Stem Cells Clin. Res, 978, pp.579~604 (2011)
- O'Meara S, "Systematic review of methods to diagnose infection in foot ulcers in diabetes", Diabet. Med, 23, pp.341~347 (2006)
- Parasuraman S, "Blood sample collection in small laboratory animals", J. Pharmacol. Pharmacother, 1, pp.87~93 (2010)
- Pelgrift RY, "Nanotechnology as a therapeutic tool to combat microbial resistance", Adv. Drug Deliv. Rev, 65, pp.1803~1815 (2013)
- Rathur HM, "Recent advances in the diagnosis and management of diabetic neuropathy", J. Bone Joint Surg, 87, pp.1605~1610 (2005)
- Regiel A, "Preparation and characterization of chitosan–silver nanocomposite films and their antibacterial activity against Staphylococcus aureus", Nanotechnology, 24, pp.015101~ (2013)
- Rey-Juradoa E, "Activity and interactions of levofloxacin, linezolid, ethambutol and amikacin in three-drug combinations against Mycobacterium tuberculosis isolates in a human macrophage model", Int. J. Antimicrob. Agents, 42, pp.524~530 (2013)
- Saha N, "Polymeric biomaterial based hydrogels for biomedical applications", J. Biomater. Nanobiotechnol, 2, pp.85~90 (2011)
- Sasikala L, "Development and evaluation of chitosan honey hydrogel sheets as wound dressing", Int. J. Pharma Bio. Sci, 6, pp.26~37 (2015)
- Savov E, "In vitro investigation of the susceptibility of Acinetobacter baumannii strains isolated from clinical specimens to ampicillin", sulbactam alone and in combination with amikacin, 2002, pp.20, 390~392
- Schwartz JA, "Prospective, non-comparative, multicenter study to investigate the effect of cadexomer iodine on bioburden load and other wound characteristics in diabetic foot ulcers", Int. Wound J, 10, pp.193~199 (2013)
- Shaheen SM, "Preparation of theophylline hydrogels of tactic poly (vinyl alcohol)", NaCl, J. Control. Release, pp.2002, 81[DOI]
- Shankar EM, "Bacterial etiology of diabetic foot infections in South India", Eur. J. Intern. Med, 16, pp.567~570 (2005)
- Smith CR, "Nephrotoxicity induced by gentamicin and amikacin", Med. J, 142, pp.85~90 (1978)
- Sondi I, "Silver nanoparticles as antimicrobial agent: case study on E. coli as a model for gram-negative bacteria", J. Colloid Interface Sci, 275, pp.177~182 (2007)
- Spilker T, "PCRbased assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients", J. Clin. Microbiol, 42, pp.2074~2079 (2004)
- Stashak TS, "Update on wound dressings: indications and best use", Clinic. Tech. Equine Pract, 3, pp.148~163 (2004)
- Sugandhi P, "Microbiological profile of bacterial pathogens from diabetic foot infections in tertiary care hospitals, salem diabetes & metabolic syndrome", Diabetes Metab. Syndr, 8, pp.129~132 (2014)
- Tally FP, "Amikacin therapy of gramnegative bacteremia", Am. J. Med, 62, pp.936~939 (1977)
- Tao Y, "Effect of chitosan on membrane permeability and cell morphology of Pseudomonas aeruginosa and Staphylococcus aureus", Carbohydr. Polym, 86, pp.969~974 (2011)
- Ternent L, "Bacterial fitness shapes the population dynamics of antibiotic-resistant and -susceptible bacteria in a model of combined antibiotic and anti-virulence treatment", J. Theor. Biol, 372, pp.1~11 (2015)
- Wei D, "The synthesis of chitosan-based silver nanoparticles and their antibacterial activity", Carbohydr. Res, 344, pp.2375~2382 (2009)
- Wild S, "Global prevalence of diabetes: estimates for the year 2000 and projections for 2030", Diabetes Care, 27, pp.1047~1053 (2004)
- Zuo GY, "Synergy of aminoglycoside antibiotics by 3-benzylchroman derivatives from the Chinese drug Caesalpinia sappan against clinical methicillin-resistant Staphylococcus aureus (MRSA)", Phytomedicine, 21, pp.936~941 (2014)
