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Comparative Analysis of the Complete Genome of Lactobacillus plantarum GB-LP2 and Potential Candidate Genes for Host Immune System Enhancement

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초록

Acute respiratory virus infectious diseases are a growing health problem, particularly among children and the elderly. Much effort has been made to develop probiotics that prevent influenza virus infections by enhancing innate immunity in the respiratory tract until vaccines are available. Lactobacillus plantarum GB-LP2, isolated from a traditional Korean fermented vegetable, has exhibited preventive effects on influenza virus infection in mice. To identify the molecular basis of this strain, we conducted a whole-genome assembly study. The single circular DNA chromosome of 3,284,304 bp was completely assembled and 3,250 proteinencoding genes were predicted. Evolutionarily accelerated genes related to the phenotypic trait of anti-infective activities for influenza virus were identified. These genes encode three integral membrane proteins, a teichoic acid export ATP-binding protein and a glucosamine - fructose-6-phosphate aminotransferase involved in host innate immunity, the nonspecific DNA-binding protein Dps, which protects bacteria from oxidative damage, and the response regulator of the three-component quorum-sensing regulatory system, which is related to the capacity of adhesion to the surface of the respiratory tract and competition with pathogens. This is the first study to identify the genetic backgrounds of the antiviral activity in L. plantarum strains. These findings provide insight into the anti-infective activities of L. plantarum and the development of preventive probiotics.

Abstract

Acute respiratory virus infectious diseases are a growing health problem, particularly among children and the elderly. Much effort has been made to develop probiotics that prevent influenza virus infections by enhancing innate immunity in the respiratory tract until vaccines are available. Lactobacillus plantarum GB-LP2, isolated from a traditional Korean fermented vegetable, has exhibited preventive effects on influenza virus infection in mice. To identify the molecular basis of this strain, we conducted a whole-genome assembly study. The single circular DNA chromosome of 3,284,304 bp was completely assembled and 3,250 proteinencoding genes were predicted. Evolutionarily accelerated genes related to the phenotypic trait of anti-infective activities for influenza virus were identified. These genes encode three integral membrane proteins, a teichoic acid export ATP-binding protein and a glucosamine - fructose-6-phosphate aminotransferase involved in host innate immunity, the nonspecific DNA-binding protein Dps, which protects bacteria from oxidative damage, and the response regulator of the three-component quorum-sensing regulatory system, which is related to the capacity of adhesion to the surface of the respiratory tract and competition with pathogens. This is the first study to identify the genetic backgrounds of the antiviral activity in L. plantarum strains. These findings provide insight into the anti-infective activities of L. plantarum and the development of preventive probiotics.

참고문헌 (35)

  1. Aziz RK, "The RAST Server: rapid annotations using subsystems technology", BMC Genomics, 9, pp.75~ (2008)
  2. Barreteau H, "Cytoplasmic steps of peptidoglycan biosynthesis", FEMS Microbiol. Rev, 32, pp.168~207 (2008)
  3. Boge T, "A probiotic fermented dairy drink improves antibody response to influenza vaccination in the elderly in two randomised controlled trials", Vaccine, 27, pp.5677~5684 (2009)
  4. Bosch A, "Viral and bacterial interactions in the upper respiratory tract", PLoS Pathog, 9, pp.e1003057~ (2013)
  5. Bron PA, "Emerging molecular insights into the interaction between probiotics and the host intestinal mucosa", Nat. Rev. Microbiol, 10, pp.66~78 (2012)
  6. Carver T, "DNAPlotter: circular and linear interactive genome visualization", Bioinformatics, 25, pp.119~120 (2009)
  7. Chiancone E, "The multifaceted capacity of Dps proteins to combat bacterial stress conditions: detoxification of iron and hydrogen peroxide and DNA binding", Biochim. Biophys. Acta, 1800, pp.798~805 (2010)
  8. Chin C-S, "Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data", Nat. Methods, 10, pp.563~569 (2013)
  9. Soo-Won Choi, "Intranasal Administration Model for Evaluating Protection Against Influenza Virus in Mice", Journal of Bacteriology and Virology, 45(1), pp.44~50 (2015), 대한미생물학회[DOI]
  10. Ferguson NM, "Strategies for mitigating an influenza pandemic", Nature, 442, pp.448~452 (2006)
  11. Ferguson NM, "Public health risk from the avian H5N1 influenza epidemic", Science, 304, pp.968~ (2004)
  12. Harata G, "Intranasally administered TMC0356 protects mice from H1N1 influenza virus infection by stimulating respiratory immune responses", World J. Microbiol. Biotechnol, 2, pp.411~416 (2011)
  13. Joseph C, "Bacterial and viral infections associated with influenza", Influenza Other Respir. Viruses, 7, pp.105~113 (2013)
  14. Kechaou N, "Identification of one novel candidate probiotic Lactobacillus plantarum strain active against influenza virus infection in mice by a large-scale screening", Appl. Environ. Microbiol, 79, pp.1491~1499 (2013)
  15. Kim KM, "An approach of orthology detection from homologous sequences under minimum evolution", Nucleic Acids Res, 36, pp.e110~e110 (2008)
  16. Löytynoja A, "Phylogeny-aware gap placement prevents errors in sequence alignment and evolutionary analysis", Science, 320, pp.1632~1635 (2008)
  17. Lebeer S, "Genes and molecules of lactobacilli supporting probiotic action", Microbiol. Mol. Biol. Rev, 72, pp.728~764 (2008)
  18. Leyer GJ, "Probiotic effects on cold and influenza-like symptom incidence and duration in children", Pediatrics, 124, pp.e172~e179 (2009)
  19. Liévin-Le Moal V, "Anti-infective activities of Lactobacillus strains in the human intestinal microbiota:from probiotics to gastrointestinal anti-infectious biotherapeutic agents", Clin. Microbiol. Rev, 27, pp.167~199 (2014)
  20. Liu Z-H, "Basic and clinical research on the regulation of the intestinal barrier by Lactobacillus and its active protein components: a review with experience of one center", Mol. Biol. Rep, 41, pp.8037~8046 (2014)
  21. Liu Z, "Involvement of the mannose receptor and p38 mitogenactivated protein kinase signaling pathway of the microdomain of the integral membrane protein after enteropathogenic Escherichia coli infection", Infect. Immun, 80, pp.1343~1350 (2012)
  22. Maldonado-Barragán A, "Knockout of three-component regulatory systems reveals that the apparently constitutive plantaricin-production phenotype shown by Lactobacillus plantarum on solid medium is regulated via quorum sensing", Int. J. Food Microbiol, 130, pp.35~42 (2009)
  23. Park M-K, "Lactobacillus plantarum DK119 as a probiotic confers protection against influenza virus by modulating innate immunity", PLoS One, 8, pp.e75368~ (2013)
  24. Richter M, "Shifting the genomic gold standard for the prokaryotic species definition", Proc. Nat. Acad. Sci. USA, 106, pp.19126~19131 (2009)
  25. Rizzardini G, "Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12® and Lactobacillus paracasei ssp. paracasei, L. casei 431® in an influenza vaccination model: a randomised, double-blind, placebo-controlled study", Br. J. Nutr, 107, pp.876~884 (2012)
  26. Sander B, "Economic evaluation of influenza pandemic mitigation strategies in the United States using a stochastic microsimulation transmission model", Value Health, 12, pp.226~233 (2009)
  27. Smith GJ, "Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic", Nature, 459, pp.1122~1125 (2009)
  28. Sturme MH, "Making sense of quorum sensing in lactobacilli: a special focus on Lactobacillus plantarum WCFS1", Microbiology, 153, pp.3939~3947 (2007)
  29. Suo C, "Effects of Lactobacillus plantarum ZJ316 on pig growth and pork quality", BMC Vet. Res, 8, pp.89~ (2012)
  30. Talavera G, "Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments", Syst. Biol, 56, pp.564~577 (2007)
  31. Tamura K, "MEGA6: molecular evolutionary genetics analysis version 6.0", Mol. Biol. Evolut, 30, pp.2725~2729 (2013)
  32. Wu S, "Web MGA: a customizable web server for fast metagenomic sequence analysis", BMC Genomics, 12, pp.444~ (2011)
  33. Yang Z., "PAML 4: phylogenetic analysis by maximum likelihood", Mol. Biol. Evol, 24, pp.1586~1591 (2007)
  34. Youn H-N, "Intranasal administration of live Lactobacillus species facilitates protection against influenza virus infection in mice", Antiviral Res, 93, pp.138~143 (2012)
  35. Zelaya H, "Nasal priming with immunobiotic Lactobacillus rhamnosus modulates inflammation–coagulation interactions and reduces influenza virus-associated pulmonary damage", Inflamm. Res, 64, pp.589~602 (2015)

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