동해 연안해역에서 분리한 해양효모의 분자계통학적 해석
Phylogeny of Marine Yeasts Isolated from Coastal Seawater in the East Sea of Korea
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BACKGROUND:Yeasts are used in a variety of industries. However,most industries are biased toward Saccharomyces cerevisiae ; so we sought to explore non-conventional yeasts (NCY). This study aimed to isolate yeasts from seawater collected from the East Sea of Korea and to analyze the NCY. METHODS AND RESULTS:We first collected seawater and performed pure isolation using four kinds of medium (GPY, DOB+CSM,DG18, and SCG). In total, 314 strains and 17 genera were isolated by ITS sequencing, including Aureobasidum pullulans (236 strains), Cryptococcus (19 strains), Cystobasidium (18 strains), and Rhodotorula (9 strains). Upon in-depth analysis, A. pullulans, the most dominant genus (236 strains), was divided into Group II (147 strains), Unknown I (8 strains), and Unknown II (49 strains). CONCLUSION: In this study, a total of 314 strains were isolated from seawater; many of these yeasts have been found and reported in seawater previously. In-depth analysis of A. pullulans, showed the dominance of Group I (21 strains) and Group II (147 strains)We also discovered Unknown I (8 strains) and Unknown II (49 strains), which have not been reported previously.
Abstract
BACKGROUND:Yeasts are used in a variety of industries. However,most industries are biased toward Saccharomyces cerevisiae ; so we sought to explore non-conventional yeasts (NCY). This study aimed to isolate yeasts from seawater collected from the East Sea of Korea and to analyze the NCY. METHODS AND RESULTS:We first collected seawater and performed pure isolation using four kinds of medium (GPY, DOB+CSM,DG18, and SCG). In total, 314 strains and 17 genera were isolated by ITS sequencing, including Aureobasidum pullulans (236 strains), Cryptococcus (19 strains), Cystobasidium (18 strains), and Rhodotorula (9 strains). Upon in-depth analysis, A. pullulans, the most dominant genus (236 strains), was divided into Group II (147 strains), Unknown I (8 strains), and Unknown II (49 strains). CONCLUSION: In this study, a total of 314 strains were isolated from seawater; many of these yeasts have been found and reported in seawater previously. In-depth analysis of A. pullulans, showed the dominance of Group I (21 strains) and Group II (147 strains)We also discovered Unknown I (8 strains) and Unknown II (49 strains), which have not been reported previously.
참고문헌 (24)
- Bhat, J., "Marine yeasts off the Indian coast. Proceedings of the Indian Academy of Sciences", Section B, Plant Sciences, 41(1), pp.9~15 (1955)
- Deak, T., "Yeast biotechnology:diversity and applications", pp.151~168 (2009), Springer Science+Business Media B.V
- Fell, J. W., "Isolation of yeasts from Biscayne bay, Florida and adjacent benthic areas", Limnology and Oceanography, 5(4), pp.366~371 (1960)
- Fell, J. W., "Isolation of heterothallic yeast strains of Metschnikowia Kamienski and their mating reactions with Chlamydozyma Wickerham spp", Antonie Van Leeuwenhoek, 34(1), pp.365~376 (1968)
- Imai, A., "Characterization of dissolved organic matter in effluents from wastewater treatment plants", Water Research, 36(4), pp.859~870 (2002)
- "한국환경농학회지", 2015, 238~243, pp.한국환경농학회, http:
- 김종식, "Yeasts Associated with Roots of the Endemic Plant Mankyua chejuense", 한국환경농학회지, 35(2), pp.137~142 (2016), 한국환경농학회[DOI]
- Kim, J. S., "Aureosurfactin and 3-deoxyaureosurfactin, novel biosurfactants produced by Aureobasidium pullulans L3-GPY", Journal of Antibiotics, 69, pp.759~761 (2016)
- 김무찬, "물벼룩(Moina macrocopa)배양을 위한 해양효모의 유효성에 대한 안전 동위원소의 증거", 미생물학회지, 42(2), pp.111~115 (2006), 한국미생물학회
- Yong-HwanKim, "CHARACTERIZATION OF DISSOLVED ORGANIC MATTER IN A SHALLOW EUTROPHIC LAKE AND INFLOWING WATERS", Environmental Engineering Research, 7(2), pp.93~101 (2002), 대한환경공학회
- Kohlmeyer, J., "Marine mycology:the higher fungi", pp.1~704 (1979), Academic Press
- Kutty, S., "Marine yeasts - a review", Yeast, 25(7), pp.465~483 (2008)
- Liu, J., "Intraspecific diversity of Aureobasidium pullulans strains from different marine environments", Journal of Ocean University Of China, 8(3), pp.241~246 (2009)
- Munn, C. B., "Marine microbiology-ecology and its applications", pp.135~136 (2004), Garland Science
- Oswal, N., "Palm oil mill effluent treatment by a tropical marine yeast", Bioresource Technology, 85(1), pp.35~37 (2002)
- Paula, C. R., "Yeasts from beaches in the southern area of Sao Paulo state Baixada Santista, Brazil", Revista de Microbiologia, 14(2), pp.136~143 (1983)
- Pitt, J. I., "The parasexual cycle in yeasts of the genus Metschnikowia", Mycologia, 62(3), pp.462~473 (1970)
- Renshaw, J. C., "Fungal siderophores: structures, functions and applications", Mycological Research, 106(10), pp.1123~1142 (2002)
- Rhishipal, R., "Selection of marine yeasts for the generation of single cell protein from prawn-shell waste", Bioresource Technology, 65(3), pp.255~256 (1998)
- Saitou, N., "The neighbor-joining method:a new method for reconstructing phylogenetic trees", Molecular biology and evolution, 4, pp.406~425 (1987)
- Satpute, S. K., "Biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms", Biotechnology Advances, 28, pp.436~450 (2010)
- Tamura K, "MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Molecular Biology and Evolution, 28, pp.2731~2739 (2011)
- Yamasato, K., "Yeast from the Pacific Ocean", Journal of General and Applied Microbiology, 20(5), pp.289~307 (1974)
- Zaky, A., "Marine yeast isolation and industrial application", FEMS Yeast Research, 14(6), pp.813~825 (2014)