Production of D-Xylonic Acid from Hemicellulose Using Artificial Enzyme Complexes
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Lignocellulosic biomass represents a potentially large resource to supply the world’s fuel and chemical feedstocks. Enzymatic bioconversion of this substrate offers a reliable strategy for accessing this material under mild reaction conditions. Owing to the complex nature of lignocellulose, many different enzymatic activities are required to function in concert to perform efficient transformation. In nature, large multienzyme complexes are known to effectively hydrolyze lignocellulose into constituent monomeric sugars. We created artificial complexes of enzymes, called rosettazymes, in order to hydrolyze glucuronoxylan, a common lignocellulose component, into its cognate sugar D-xylose and then further convert the D-xylose into D-xylonic acid, a Department of Energy top-30 platform chemical. Four different types of enzymes (endoxylanase, α-glucuronidase, β-xylosidase, and xylose dehydrogenase) were incorporated into the artificial complexes. We demonstrated that tethering our enzymes in a complex resulted in significantly more activity (up to 71%) than the same amount of enzymes free in solution. We also determined that varying the enzyme composition affected the level of complex-related activity enhancement as well as overall yield.
Abstract
Lignocellulosic biomass represents a potentially large resource to supply the world’s fuel and chemical feedstocks. Enzymatic bioconversion of this substrate offers a reliable strategy for accessing this material under mild reaction conditions. Owing to the complex nature of lignocellulose, many different enzymatic activities are required to function in concert to perform efficient transformation. In nature, large multienzyme complexes are known to effectively hydrolyze lignocellulose into constituent monomeric sugars. We created artificial complexes of enzymes, called rosettazymes, in order to hydrolyze glucuronoxylan, a common lignocellulose component, into its cognate sugar D-xylose and then further convert the D-xylose into D-xylonic acid, a Department of Energy top-30 platform chemical. Four different types of enzymes (endoxylanase, α-glucuronidase, β-xylosidase, and xylose dehydrogenase) were incorporated into the artificial complexes. We demonstrated that tethering our enzymes in a complex resulted in significantly more activity (up to 71%) than the same amount of enzymes free in solution. We also determined that varying the enzyme composition affected the level of complex-related activity enhancement as well as overall yield.
참고문헌 (49)
- Ren N, "Bioconversion of lignocellulosic biomass to hydrogen: potential and challenges", Biotechnol. Adv, 27, pp.1051~1060 (2009)
- Kircher M., "Sustainability of biofuels and renewable chemicals production from biomass", Curr. Opin. Chem. Biol, 29, pp.26~31 (2015)
- Tyner WE., "Biofuels and food prices: separating wheat from chaff", Glob. Food Sec, 2, pp.126~130 (2013)
- Balan V., "Current challenges in commercially producing biofuels from lignocellulosic biomass", ISRN Biotechnol, 2014, pp.463074~ (2014)
- McCann MC, "Biomass recalcitrance: a multi-scale, multi-factor, and conversion-specific property", J. Exp. Bot, 66, pp.4109~4118 (2015)
- Zhang GC, "Combining C6 and C5 sugar metabolism for enhancing microbial bioconversion", Curr. Opin. Chem. Biol, 29, pp.49~57 (2015)
- Guerriero G, "Lignocellulosic biomass: biosynthesis, degradation, and industrial utilization", Eng. Life Sci, 16, pp.1~16 (2016)
- Rennie EA, "Xylan biosynthesis", Curr. Opin. Biotechnol, 26, pp.100~107 (2014)
- Numan MT, "α-L-Arabinofuranosidases: the potential applications in biotechnology", J. Ind. Microbiol. Biotechnol, 33, pp.247~260 (2006)
- Poutanen K, "Enzymes in Biomass Conversion", pp.426~436 (1991), American Chemical Society
- Dutta S, "Enzymatic breakdown of biomass:Enzyme active sites, immobilization, and biofuel production", Green Chem, 16, pp.4615~4626 (2014)
- Hong-chen Zheng, "Purification and Characterization of a Thermostable Xylanase from Paenibacillus sp. NF1 and its Application in Xylooligosaccharides Production", Journal of Microbiology and Biotechnology, 24(4), pp.489~496 (2014), 한국미생물·생명공학회[DOI]
- 이선화, "Cloning and Characterization of a Multidomain GH10 Xylanase from Paenibacillus sp. DG-22", Journal of Microbiology and Biotechnology, 24(11), pp.1525~1535 (2014), 한국미생물·생명공학회[DOI]
- 이선화, "Cloning, Expression, and Characterization of a Thermostable GH51 α-L-Arabinofuranosidase from Paenibacillus sp. DG-22", Journal of Microbiology and Biotechnology, 24(2), pp.236~244 (2014), 한국미생물·생명공학회[DOI]
- Fei Li, "Improvement of the Optimum pH of Aspergillus niger Xylanase towards an Alkaline pH by Site-Directed Mutagenesis", Journal of Microbiology and Biotechnology, 25(1), pp.11~17 (2015), 한국미생물·생명공학회[DOI]
- "Top Value Added Chemicals From Biomass. Volume I—Results of Screening for Potential Candidates from Sugars and Synthesis Gas" (2004)[URL]
- Chun BW, "The development of cement and concrete additive: based on xylonic acid derived via bioconversion of xylose", Appl. Biochem. Biotechnol, 131, pp.645~658 (2006)
- Zamora F, "Stereoregular copolyamides derived from D-xylose and L-arabinose", Macromolecules, 33, pp.2030~2038 (2000)
- Niu W, "Microbial synthesis of the energetic material precursor 1,2,4-butanetriol", J. Am. Chem. Soc, 125, pp.12998~12999 (2003)
- Sun L, "Synthetic pathway optimization for improved 1,2,4-butanetriol production", J. Ind. Microbiol. Biotechnol, 43, pp.67~78 (2016)
- Bayer EA, "The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides", Annu. Rev. Microbiol, 58, pp.521~554 (2004)
- Fontes CM, "Cellulosomes: highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates", Annu. Rev. Biochem, 79, pp.655~681 (2010)
- Raman B, "Impact of pretreated switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis", PLoS One, 4, pp.e5271~ (2009)
- Borne R, "Unraveling enzyme discrimination during cellulosome assembly independent of cohesin-dockerin affinity", FEBS J, 280, pp.5764~5779 (2013)
- McClendon SD, "Designer xylanosomes: protein nanostructures for enhanced xylan hydrolysis", Appl. Biochem. Biotechnol, 167, pp.395~411 (2012)
- Morais S, "Deconstruction of lignocellulose into soluble sugars by native and designer cellulosomes", MBio, 3, pp.e00508~e00512 (2012)
- Liang Y, "Engineered pentafunctional minicellulosome for simultaneous saccharification and ethanol fermentation in Saccharomyces cerevisiae", Appl. Environ. Microbiol, 80, pp.6677~6684 (2014)
- Jingshen Ou, "Incorporation of Nasutitermes takasagoensis Endoglucanase into Cell Surface-Displayed Minicellulosomes in Pichia pastoris X33", Journal of Microbiology and Biotechnology, 24(9), pp.1178~1188 (2014), 한국미생물·생명공학회[DOI]
- Fan LH, "Selfsurface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production", Proc. Natl. Acad. Sci. USA, 109, pp.13260~13265 (2012)
- Stern J, "Adaptor scaffoldins: an original strategy for extended designer cellulosomes, inspired from nature", MBio, 7, pp.e00083~e00016 (2016)
- Wheeldon I, "Substrate channelling as an approach to cascade reactions", Nat. Chem, 8, pp.299~309 (2016)
- Mitsuzawa S, "The rosettazyme: a synthetic cellulosome", J. Biotechnol, 143, pp.139~144 (2009)
- Kagawa HK, "The composition, structure and stability of a group II chaperonin are temperature regulated in a hyperthermophilic archaeon", Mol. Microbiol, 48, pp.143~156 (2003)
- McMillan RA, "A self-assembling protein template for constrained synthesis and patterning of nanoparticle arrays", J. Am. Chem. Soc, 127, pp.2800~2801 (2005)
- McMillan RA, "Ordered nanoparticle arrays formed on engineered chaperonin protein templates", Nat. Mater, 1, pp.247~252 (2002)
- Paavola CD, "A versatile platform for nanotechnology based on circular permutation of a chaperonin protein", Nanotechnology, 17, pp.1171~1176 (2006)
- Mishra S, "Transcription of Clostridium thermocellum endoglucanase genes celF and celD", J. Bacteriol, 173, pp.80~85 (1991)
- Studier FW., "Protein production by auto-induction in high density shaking cultures", Protein Expr. Purif, 41, pp.207~234 (2005)
- Lee CC, "Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme", Curr. Microbiol, 57, pp.301~305 (2008)
- Milner Y, "A copper reagent for the determination of hexuronic acids and certain ketohexoses", Carbohydr. Res, 4, pp.359~361 (1967)
- Wagschal K, "Enzyme-coupled assay for β-xylosidase hydrolysis of natural substrates", Appl. Environ. Microbiol, 71, pp.5318~5323 (2005)
- Wagschal K, "Biochemical characterization of uronate dehydrogenases from three pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans", Enzyme Microb. Technol, 69, pp.62~68 (2015)
- Lee CC, "Isolation and characterization of a cold-active xylanase enzyme from Flavobacterium sp", Curr. Microbiol, 52, pp.112~116 (2006)
- Pell G, "The mechanisms by which family 10 glycoside hydrolases bind decorated substrates", J. Biol. Chem, 279, pp.9597~9605 (2004)
- Lee CC, "Isolation of α-glucuronidase enzyme from a rumen metagenomic library", Protein J, 31, pp.206~211 (2012)
- Jordan DB, "β-D-Xylosidase from Selenomonas ruminantium:catalyzed reactions with natural and artificial substrates", Appl. Biochem. Biotechnol, 146, pp.137~149 (2008)
- Stephens C, "Genetic analysis of a novel pathway for Dxylose metabolism in Caulobacter crescentus", J. Bacteriol, 189, pp.2181~2185 (2007)
- Liu H, "Highyield producti on of D-xylonic acid from Dxylose using engineered Escherichia coli", Bioresour. Technol, 115, pp.244~248 (2012)
- Toivari M, "Metabolic engineering of Saccharomyces cerevisiae for bioconversi on of D-xylose to D-xylonate", Metab. Eng, 14, pp.427~436 (2012)