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Engineering of Sulfolobus acidocaldarius for Hemicellulosic Biomass Utilization

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The saccharification of cellulose and hemicellulose is essential for utilizing lignocellulosic biomass as a biofuel. While cellulose is composed of glucose only, hemicelluloses are composed of diverse sugars such as xylose, arabinose, glucose, and galactose. Sulfolobus acidocaldarius is a good potential candidate for biofuel production using hemicellulose as this archaeon simultaneously utilizes various sugars. However, S. acidocaldarius has to be manipulated because the enzyme that breaks down hemicellulose is not present in this species. Here, we engineered S. acidocaldarius to utilize xylan as a carbon source by introducing xylanase and β-xylosidase. Heterologous expression of β-xylosidase enhanced the organism’s degradability and utilization of xylooligosaccharides (XOS), but the mutant still failed to grow when xylan was provided as a carbon source. S. acidocaldarius exhibited the ability to degrade xylan into XOS when xylanase was introduced, but no further degradation proceeded after this sole reaction. Following cell growth and enzyme reaction, S. acidocaldarius successfully utilized xylan in the synergy between xylanase and β-xylosidase.

Abstract

The saccharification of cellulose and hemicellulose is essential for utilizing lignocellulosic biomass as a biofuel. While cellulose is composed of glucose only, hemicelluloses are composed of diverse sugars such as xylose, arabinose, glucose, and galactose. Sulfolobus acidocaldarius is a good potential candidate for biofuel production using hemicellulose as this archaeon simultaneously utilizes various sugars. However, S. acidocaldarius has to be manipulated because the enzyme that breaks down hemicellulose is not present in this species. Here, we engineered S. acidocaldarius to utilize xylan as a carbon source by introducing xylanase and β-xylosidase. Heterologous expression of β-xylosidase enhanced the organism’s degradability and utilization of xylooligosaccharides (XOS), but the mutant still failed to grow when xylan was provided as a carbon source. S. acidocaldarius exhibited the ability to degrade xylan into XOS when xylanase was introduced, but no further degradation proceeded after this sole reaction. Following cell growth and enzyme reaction, S. acidocaldarius successfully utilized xylan in the synergy between xylanase and β-xylosidase.

참고문헌 (37)

  1. Serrano-Ruiz JC, "Transformations of biomass-derived platform molecules : from high added-value chemicals to fuels via aqueous-phase processing", Chem. Soc. Rev, 40, pp.5266~5281 (2011)
  2. Martins F, "Analysis of fossil fuel energy consumption and environmental impacts in European countries", Energies, 12, pp.964~ (2019)
  3. Change C, "IPCC fourth assessment report", Phys. Sci. Basis, 2, pp.580~595 (2007)
  4. Nel WP, "Implications of fossil fuel constraints on economic growth and global warming", Energy Policy, 37, pp.166~180 (2009)
  5. Gençsü I, "Phasing out public financial flows to fossil fuel production in Europe", Clim. Policy, 20, pp.1010~1023 (2020)
  6. Ayling J, "Non-state governance and climate policy : the fossil fuel divestment movement", Clim. Policy, 17, pp.131~149 (2017)
  7. Bergman N, "Impacts of the fossil fuel divestment movement : effects on finance, policy and public discourse", Sustainability, 10, pp.2529~ (2018)
  8. Zoghlami A, "Lignocellulosic biomass : understanding recalcitrance and predicting hydrolysis", Front. Chem, 7, pp.874~ (2019)
  9. Singhvi MS, "Lignocellulosic biomass : hurdles and challenges in its valorization", Appl. Microbiol. Biotechnol, 103, pp.9305~9320 (2019)
  10. Houfani AA, "Insights from enzymatic degradation of cellulose and hemicellulose to fermentable sugars–a review", Biomass. Bioenerg, 134, pp.105481~ (2020)
  11. Kumar R, "Bioconversion of lignocellulosic biomass : biochemical and molecular perspectives", J. Ind. Microbiol. Biotechnol, 35, pp.377~391 (2008)
  12. Limayem A, "Lignocellulosic biomass for bioethanol production : current perspectives, potential issues and future prospects", Prog. Energy Combust. Sci, 38, pp.449~467 (2012)
  13. Scheller HV, "Hemicelluloses", Annu. Rev. Plant Biol, 61, pp.263~289 (2010)
  14. Schocke L, "Thermoacidophilic Sulfolobus species as source for extremozymes and as novel archaeal platform organisms", Curr. Opin. Biotechnol, 59, pp.71~77 (2019)
  15. Quehenberger J, "Sulfolobus-A potential key organism in future biotechnology", Front. Microbiol, 8, pp.2474~ (2017)
  16. Joshua CJ, "Absence of diauxie during simultaneous utilization of glucose and xylose by Sulfolobus acidocaldarius", J. Bacteriol, 193, pp.1293~1301 (2011)
  17. Brock TD, "Sulfolobus : a new genus of sulfur-oxidizing bacteria living at low pH and high temperature", Arch. Microbiol, 84, pp.54~68 (1972)
  18. Wagner M, "Versatile genetic tool box for the crenarchaeote Sulfolobus acidocaldarius", Front. Microbiol, 3, pp.214~ (2012)
  19. Chen L, "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota", J. Bacteriol, 187, pp.4992~4999 (2005)
  20. Michaela Wagner, "Sulfolobus acidocaldarius Transports Pentoses via a Carbohydrate Uptake Transporter 2 (CUT2)-Type ABC Transporter and Metabolizes Them through the Aldolase-Independent Weimberg Pathway", Applied and Environmental Microbiology, 84(3) (2018), American Society for Microbiology[DOI]
  21. Nunn CE, "Metabolism of pentose sugars in the hyperthermophilic archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius", J. Biol. Chem, 285, pp.33701~33709 (2010)
  22. Cannio R, "A xylan-degrading strain of Sulfolobus solfataricus : isolation and characterization of the xylanase activity", Extremophiles, 8, pp.117~124 (2004)
  23. Morana A, "Gene cloning and expression in Escherichia coli of a bi-functional β-Dxylosidase", α-L-arabinosidase from Sulfolobus solfataricus involved in xylan degradation, 2007, pp.11, 123~132
  24. Maurelli L, "Evidence that the xylanase activity from Sulfolobus solfataricus Oα is encoded by the endoglucanase precursor gene(sso1354)and characterization of the associated cellulase activity", Extremophiles, 12, pp.689~700 (2008)
  25. Van Der Kolk N, "Identification of XylR, the activator of arabinose", xylose inducible regulon in Sulfolobus acidocaldarius and its application for homologous protein expression, 2020, pp.11, 1066~
  26. Prangishvili DA, "A restriction endonuclease SuaI from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius", FEBS Lett, 192, pp.57~60 (1985)
  27. Kurosawa N, "Homologous recombination of exogenous DNA with the Sulfolobus acidocaldarius genome : properties and uses", FEMS Microbiol. Lett, 253, pp.141~149 (2005)
  28. Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem, 72, pp.248~254 (1976)
  29. Choi KH, "Identification and characterization of MalA in the maltose", maltodextrin operon of Sulfolobus acidocaldarius DSM639, 2013, pp.195, 1789~1799
  30. Reiffová K, "Thin-layer chromatography analysis of fructooligosaccharides in biological samples", J. Chromatogr. A, 1110, pp.214~221 (2006)
  31. Chen C-H, "Engineer multi-functional cellulase", xylanase, Bioresour. Technol. Rep, pp.2019, 5[DOI]
  32. She Q, "The complete genome of the crenarchaeon Sulfolobus solfataricus P2", Proc. Natl. Acad. Sci. USA, 98, pp.7835~7840 (2001)
  33. Eichler J, "Post-translational protein modification in Archaea", Microbiol. Mol. Biol. Rev, 69, pp.393~425 (2005)
  34. Meyer BH, "Hot and sweet : protein glycosylation in Crenarchaeota", Biochem. Soc. Trans, 41, pp.384~392 (2013)
  35. Girfoglio M, "Cellulose degradation by Sulfolobus solfataricus requires a cell-anchored endo-β-1–4-glucanase", J. Bacteriol, 194, pp.5091~5100 (2012)
  36. Chen L, "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota", J. Bacteriol, 187, pp.4992~4999 (2005)
  37. Berkner S, "Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the(cren-)archaea", Nucleic Acids Res, 35, pp.e88~ (2007)

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