본문 바로가기
KERD
논문KCI 등재

Evaluation of the Synergistic Effect of Mixed Cultures of White-Rot Fungus Pleurotus ostreatus and Biosurfactant-Producing Bacteria on DDT Biodegradation

0

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류생물학

초록

DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane) is one of the organic synthetic pesticides that has many negative effects for human health and the environment. The purpose of this study was to investigate the synergistic effect of mixed cutures of white-rot fungus, Pleurotus ostreatus, and biosurfactant-producing bacteria, Pseudomonas aeruginosa and Bacillus subtilis, on DDT biodegradation. Bacteria were added into the P. ostreatus culture (mycelial wet weight on average by 8.53 g) in concentrations of 1, 3, 5, and 10 ml (1 ml ≈ 1.25 × 109 bacteria cells/ml culture). DDT was degraded to approximately 19% by P. ostreatus during the 7-day incubation period. The principal result of this study was that the addition of 3 ml of P. aeruginosa into P. ostreatus culture gave the highest DDT degradation rate (approximately 86%) during the 7-day incubation period. This mixed culture combination of the fungus and bacteria also gave the best ratio of optimization of 1.91. DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2-bis(4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by P. ostreatus and P. aeruginosa. The results of this study indicate that P. aeruginosa has a synergistic relationship with P. ostreatus and can be used to optimize the degradation of DDT by P. ostreatus.

Abstract

DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane) is one of the organic synthetic pesticides that has many negative effects for human health and the environment. The purpose of this study was to investigate the synergistic effect of mixed cutures of white-rot fungus, Pleurotus ostreatus, and biosurfactant-producing bacteria, Pseudomonas aeruginosa and Bacillus subtilis, on DDT biodegradation. Bacteria were added into the P. ostreatus culture (mycelial wet weight on average by 8.53 g) in concentrations of 1, 3, 5, and 10 ml (1 ml ≈ 1.25 × 109 bacteria cells/ml culture). DDT was degraded to approximately 19% by P. ostreatus during the 7-day incubation period. The principal result of this study was that the addition of 3 ml of P. aeruginosa into P. ostreatus culture gave the highest DDT degradation rate (approximately 86%) during the 7-day incubation period. This mixed culture combination of the fungus and bacteria also gave the best ratio of optimization of 1.91. DDD (1,1-dichloro-2,2-bis(4-chlorophenyl) ethane), DDE (1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene), and DDMU (1-chloro-2,2-bis(4-chlorophenyl) ethylene) were detected as metabolic products from the DDT degradation by P. ostreatus and P. aeruginosa. The results of this study indicate that P. aeruginosa has a synergistic relationship with P. ostreatus and can be used to optimize the degradation of DDT by P. ostreatus.

참고문헌 (76)

  1. Boul HL., "DDT residues in the environment - a review with a New Zealand perspective", N.Z. J. Agric. Res, 38, pp.257~277 (1995)
  2. Busvine JR, "DDT: fifty years for good or ill", Pestic. Outlook, 1, pp.4~8 (1989)
  3. Foght J, "Bioremediation of DDT-contaminated soils: a review", Bioremediat. J, 5, pp.225~246 (2001)
  4. Kale SP, "Studies on degradation of 14C- DDT in the marine environment", Chemosphere, 39, pp.959~968 (1999)
  5. Aislabie JM, "Microbial degradation of DDT and its residues - a review", N.Z. J. Agric. Res, 40, pp.269~282 (1997)
  6. Simonich SL, "Global distribution of persistent organochlorine compounds", Science, 269, pp.1851~1854 (1995)
  7. Purnomo AS, "Degradation of 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) by brown-rot fungi", J. Biosci. Bioeng, 105, pp.614~621 (2008)
  8. Purnomo AS, "Application of mushroom waste medium from Pleurotus ostreatus for bioremediation of DDT-contaminated soil", Int. Biodeterior. Biodegrad, 64, pp.397~402 (2010)
  9. Abalos A, "Physicochemical and antimicrobial properties of new rhamnolipids produced by Pseudomonas aeruginosa AT10 from soybean oil refinery wastes", Langmuir, 17, pp.1367~1371 (2001)
  10. Datta S., "Optimization of culture conditions for biosurfactant production from Pseudomonas aeruginosa OCD1", J. Adv. Sci. Res, 2, pp.32~36 (2011)
  11. Guerra-Santos L, "Pseudomonas aeruginosa biosurfactant production in continuous culture with glucose as carbon source", Appl. Environ. Microbiol, 48, pp.301~305 (1984)
  12. Rahman KSM, "Rhamnolipid biosurfactant production by strains of Pseudomonas aeruginosa using low-cost raw materials", Biotechnol. Prog, 18, pp.1277~1281 (2002)
  13. Silva SNRL, "Glycerol as substrate for the production of biosurfactant by Pseudomonas aeruginosa UCP0992", Colloids Surf. B Biointerfaces, 79, pp.174~183 (2010)
  14. Yin H, "Characteristics of biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater", Process Biochem, 44, pp.302~308 (2009)
  15. Zhang C, "Isomerization and biodegradation of beta-cypermethrin by Pseudomonas aeruginosa CH7 with biosurfactant production", Bioresour. Technol, 102, pp.7139~7146 (2012)
  16. Gudiña EJ, "Potential therapeutic applications of biosurfactants", Trends Pharmacol. Sci, 34, pp.667~675 (2013)
  17. Pornsunthorntawee N, "Isolation and comparison of biosurfactants produced by Bacillus subtilis Pt2 and Pseudomonas aeruginosa SP4 for microbial surfactant-enhanced oil recovery", Biochem. Eng. J, 42, pp.172~179 (2008)
  18. Zouari R, "Optimization of Bacillus subtilis SPB1 biosurfactant production under solidstate fermentation using by-products of a traditional olive mill factory", Achiev. Life Sci, 8, pp.162~169 (2014)
  19. Bidlan R., "Studies on DDT degradation by bacterial strains", PhD, pp.2003
  20. Golovleva L, "Degradation of DDT and its analogs by Pseudomonas aeruginosa 640x", Biol. Bull. Acad. Sci. USSR, 7, pp.143~151 (1980)
  21. Bumpus JA, "Biotransformations: Microbial Degradation of Health-Risk Compounds", pp.157~175 (1995), Elsevier Science
  22. Cho EA, "Decolorization of indigo carmine by laccase displayed on Bacillus subtilis spores", Enzyme Microb. Technol, 49, pp.100~104 (2011)
  23. Farzaneh M, "Aflatoxin B1degradation by Bacillus subtilis UTBSP1 isolated from pistachio nuts of Iran", Food Control, 23, pp.100~106 (2012)
  24. Sompornpailin D, "Diethyl phthalate degradation by the freeze-dried, entrapped Bacillus subtilis strain 3C3", Int. Biodeterior. Biodegrad, 91, pp.138~147 (2014)
  25. Johnson BT, "Biomagnification of p,p’-DDT and methoxychlor by bacteria", Appl. Microbiol, 26, pp.66~71 (1973)
  26. Langlois BE, "Some factors affecting degradation of organochlorine pesticide by bacteria", J. Dairy Sci, 53, pp.1671~1675 (1970)
  27. Purnomo AS, "Involvement of Fenton reaction in DDT degradation by brown-rot fungi", Int. Biodeterior. Biodegrad, 64, pp.560~565 (2010)
  28. Purnomo AS, "Biotransformation of heptachlor and heptachlor epoxide by white-rot fungus Pleurotus ostreatus", Int. Biodeterior. Biodegrad, 82, pp.40~44 (2013)
  29. Purnomo AS, "Biodegradation of aldrin and dieldrin by white-rot fungus Pleurotus ostreatus", Curr. Microbiol, 74, pp.320~324 (2017)
  30. Wahyuni S, "Purification and characterization of thermostable chitinase from Bacillus SW41 for chitin oligomer production", Asian J. Chem, 28, pp.2731~2736 (2016)
  31. Purnomo AS, "Bioremediation of DDT contaminated soil using brown-rot fungi", Int. Biodeterior. Biodegrad, 65, pp.691~695 (2011)
  32. Purnomo AS, "DDT degradation potential of cattle manure compost", Chemosphere, 80, pp.619~624 (2010)
  33. Purnomo AS, "Basic studies and applications on bioremediation of DDT: a review", Int. Biodeterior. Biodegrad, 65, pp.921~930 (2011)
  34. Purnomo AS, "Biodegradation of heptachlor and heptachlor epoxidecontaminated soils by white-rot fungal inocula", Environ. Sci. Pollut. Res, 21, pp.11305~11312 (2014)
  35. Subba Rao RV, "Bacterial and fungal cometabolism of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane (DDT) and its breakdown products", Appl. Environ. Microbiol, 49, pp.509~516 (1985)
  36. Arisoy M., "Biodegradation of chlorinated organic compounds by white-rot fungi", Bull. Environ. Contam. Toxicol, 60, pp.872~876 (1998)
  37. by the white rot fungus Phanerochaete chrysosporium, "Appl. Environ. Microbiol", 1987, 2001~2008
  38. Shah MM, "Use of white rot fungi in the degradation of environmental chemicals", Toxicol. Lett, 64-65, pp.493~501 (1992)
  39. Xiao P, "A novel metabolic pathway for biodegradation of DDT by white rot fungi, Phlebia lindtneri and Phlebia brevispora", Biodegradation, 22, pp.859~867 (2011)
  40. Wedemeyer G, "Dechlorination of DDT by Aerobacter aerogenes", Science, 152, pp.647~ (1966)
  41. Wedemeyer G., "Dechlorination of 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane by Aerobacter aerogenes", Appl. Microbiol, 15, pp.569~574 (1967)
  42. Patil KC, "Degradation of endrin, aldrin, and DDT by soil microorganisms", Appl. Environ. Microbiol, 19, pp.879~881 (1970)
  43. Pfaender FK, "Extensive microbial degradation of DDT in vitro and DDT metabolism by natural communities", J. Agric. Food Chem, 20, pp.842~846 (1972)
  44. Focht DD, "DDT metabolites and analogs: ring fission by Hydrogenomonas", Science, 170, pp.91~92 (1970)
  45. Focht DD, "Aerobic cometabolism of DDT analogues by Hydrogenomonas sp", J. Agric. Food Chem, 19, pp.20~22 (1971)
  46. Nadeau LJ, "Aerobic degradation of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane (DDT) by Alcaligenes eutrophus A5", Appl. Environ. Microbiol, 60, pp.51~55 (1994)
  47. Pesce SF, "Biodegradation of lindane by a native bacterial consortium isolated from contaminated river sediment", Int. Biodeterior. Biodegrad, 54, pp.255~260 (2004)
  48. Kamanavalli CM, "Biodegradation of DDT by a Pseudomonas species", Curr. Microbiol, 48, pp.10~13 (2004)
  49. Fang H, "Characterization of a bacterial strain capable of degrading DDT congeners and its use in bioremediation of contaminated soil", J. Hazard. Mater, 184, pp.281~289 (2010)
  50. Kantachote D, "DDT resistance and transformation by different microbial strains isolated from DDT-contaminated soils and compost materials", Compost Sci. Util, 11, pp.300~310 (2003)
  51. Bajaj A, "Isolation and functional analysis of a glycolipid producing Rhodococcus sp. strain IITR03 with potential for degradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (DDT)", Bioresour. Technol, 167, pp.398~406 (2014)
  52. Wang S, "Case study of the relationship between fungi and bacteria associated with high-molecular-weight polycyclic aromatic hydrocarbon degradation", J. Biosci. Bioeng, 113, pp.624~630 (2012)
  53. Hai FI, "Pesticide removal by a mixed culture of bacteria and white-rot fungi", J. Taiwan Inst. Chem. Eng, 43, pp.459~462 (2012)
  54. Ge S, "Effects of exogenous aerobic bacteria on methane production and biodegradation of municipal solid waste in bioreactors", Waste Manag, 55, pp.93~98 (2016)
  55. Mata-Sandoval JC, "Influence of rhamnolipids and Triton X-100 on the biodegradation of three pesticides in aqueous and soil slurries", J. Agric. Food Chem, 49, pp.3296~3303 (2001)
  56. Deepika KV, "Optimization of rhamnolipid biosurfactant production by mangrove sediment bacterium Pseudomonas aeruginosa KVD-HR42 using response surface methodology", Biocatal. Agric. Biotechnol, 5, pp.38~47 (2016)
  57. El-Sheshtawy HS, "Selection of Pseudomonas aeruginosa for biosurfactant production and studies of its antimicrobial activity", Egypt. J. Petrol, 23, pp.1~6 (2014)
  58. Bezza FA, "Production and applications of lipopeptide biosurfactant for bioremediation and oil recovery by Bacillus subtilis CN2", Biochem. Eng. J, 101, pp.168~178 (2015)
  59. Wang T, "Natural products from Bacillus subtilis with antimicrobial properties", Chinese J. Chem. Eng, 23, pp.744~754 (2015)
  60. Zheng G, "Oil-ni-water microemulsions enhance the biodegradation of DDT by Phanerochaete chrysosporium", Bioresour. Technol, 126, pp.397~403 (2012)
  61. Betancur-Corredor B, "Evaluation of biostimulation and Tween 80 addition for the bioremediation of long-term DDT-contaminated soil", J. Environ. Sci, 28, pp.101~109 (2015)
  62. Buswell JA, "Mushroom Biology and Mushroom Products", pp.141~150 (1993), The Chinese University Press
  63. Masaphy S, "Microsomal and cytosolic cytochrome-P450 mediated benzo(a)pyrene hydroxylation in Pleurotus pulmonarius", Biotechnol. Lett, 17, pp.969~974 (1995)
  64. Masaphy S, "Evidence for cytochrome P-450 and P-450-mediated benzo(a)pyrene hydroxylation in the white rot fungus Phanerochaete chrysosporium", FEMS Microbiol. Lett, 135, pp.51~55 (1996)
  65. Bezalel L, "Initial oxidation products in the metabolism of pyrene, anthracene, fluorene, and dibenzothiophene by the white rot fungus Pleurotus ostreatus", Appl. Environ. Microbiol, 62, pp.2554~2559 (1996)
  66. Kamei I, "Biotransformation of dichloro-, trichloro-, and tetrachlorodibenzo-p-dioxin by the white-rot fungus Phlebia lindtneri", Appl. Microbiol. Biotechnol, 68, pp.560~566 (2005)
  67. Kamei I, "Fungal bioconversion of toxic polychlorinated biphenyls by white-rot fungus, Phlebia brevispora", Appl. Microbiol. Biotechnol, 73, pp.932~940 (2006)
  68. Mori T, "Oxidation of chlorinated dibenzo-pdioxin and dibenzofuran by white-rot fungus, Phlebia lindtneri", FEMS Microbiol. Lett, 216, pp.223~227 (2002)
  69. Mori T, "Fungal hydroxylation of polychlorinated naphthalenes with chlorine migration by wood rotting fungi", Chemosphere, 77, pp.1230~1235 (2009)
  70. Hiratsuka N, "Degradation of diphenyl ether herbicides by the lignin-degrading basidiomycete Coriolus versicolor", Appl. Microbiol. Biotechnol, 57, pp.563~571 (2001)
  71. Cùany A, "Characterization of microsomal oxidative activities in a wild-type and in a DDT resistant strain of Drosophila melanogaster", Pestic. Biochem. Physiol, 37, pp.293~302 (1990)
  72. Joußen N, "Metabolism of imidacloprid and DDT by P450 CYP6G1expressed in cell cultures of Nicotiana tabacum suggests detoxification of these insecticides in Cyp6g1-overexpressing strains of Drosophila melanogaster, leading to resistance", Pest Manag. Sci, 64, pp.65~73 (2008)
  73. Suhara H, "Degradation of chlorinated pesticide DDT by litter-decomposing basidiomycetes", Biodegradation, 22, pp.1075~1086 (2011)
  74. Bidlan M, "Aerobic degradation of dichlorodiphenyltrichloroethane (DDT) by Serratia marcescens DT-1P", Process Biochem, 38, pp.49~56 (2002)
  75. Li FB, "Enhanced reductive dechlorination of DDT in an anaerobic system of dissimilatory iron-reducing bacteria and iron oxide", Environ. Pollut, 158, pp.1733~1740 (2010)
  76. Bao P, "Dechlorination of p,p’-DDTs coupled with sulfate reduction by novel sulfate-reducing bacterium Clostridium sp BXM", Environ. Pollut, 162, pp.303~310 (2012)

관련 논문AI추천

Ralstonia pickettii Enhance the DDT Biodegradation by Pleurotus eryngiiAdi Setyo Purnomo, Diana Maulianawati, Ichiro Kamei한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2019 · 제29권 제9호Indirect Bacterial Effect Enhanced Less Recovery of Neonicotinoids by Improved Activities of White-Rot Fungus Phlebia brevisporaIchiro Kamei, Joy L. Harry-Asobara한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2019 · 제29권 제5호Biodegradation of Organochlorine Insecticide Endosulfan by the Fungus Eutypella sp. KEF-1권기석, 김장억, 박상열, 신기선, 이중복, 전춘표한국환경농학회 · 한국환경농학회지2011 · 제30권 제2호Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade FenvalerateCai Ting, Lei Dan, Tang Jie, Wu Min, Ye Hong, Zeng Chaoyi, Zhang Qing, Zhou Xuerui한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2022 · 제32권 제2호Characterization of Bacillus zhangzhouensis Strain P with Cypermethrin-Degrading PotentialIskandarova Umida, Kosimov Diyorbek, Muminov Muzaffar, Zaynitdinova Lyudmila한국미생물·생명공학회 · 한국미생물·생명공학회지2025 · 제53권 제4호유기염소계 농약 endosulfan을 분해하는 미생물의 분리 및 분해 특성곽윤영, 김원찬, 김장억, 김태화, 박종우, 소재현, 신재호, 신현수, 이인구한국환경농학회 · 한국환경농학회지2008 · 제27권 제3호

이 간행물의 다른 논문 (11건)