The Bactericidal Effect of High Temperature Is an Essential Resistance Mechanism of Chicken Macrophage against Brucella abortus Infection
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Knowledge of avian host responses to brucellosis is critical to understanding how birds resist this infection; however, this mechanism is not well established. On the other hand, temperature has a major involvement in the physiology of living organisms, and cell death induced by heat is attributed to protein denaturation. This study demonstrates the direct bactericidal effect of a high temperature (41ºC) on Brucella abortus that resulted in the gradual reduction of intracellular bacteria and inhibited bacterial growth within avian macrophage HD11 in an increasing period of time. On the other hand, this study also revealed that high temperature does not affect the rate of bacterial uptake, as confirmed by the bacterial adherence assay. No significant difference was observed in the expression of target genes between infected and uninfected cells for both temperatures. This study suggests the susceptibility of B. abortus to bacterial death under a high temperature with an increased period of incubation, leading to suppression of bacterial growth.
Abstract
Knowledge of avian host responses to brucellosis is critical to understanding how birds resist this infection; however, this mechanism is not well established. On the other hand, temperature has a major involvement in the physiology of living organisms, and cell death induced by heat is attributed to protein denaturation. This study demonstrates the direct bactericidal effect of a high temperature (41ºC) on Brucella abortus that resulted in the gradual reduction of intracellular bacteria and inhibited bacterial growth within avian macrophage HD11 in an increasing period of time. On the other hand, this study also revealed that high temperature does not affect the rate of bacterial uptake, as confirmed by the bacterial adherence assay. No significant difference was observed in the expression of target genes between infected and uninfected cells for both temperatures. This study suggests the susceptibility of B. abortus to bacterial death under a high temperature with an increased period of incubation, leading to suppression of bacterial growth.
참고문헌 (27)
- Zekarias B, "Immunological basis of differences in disease resistance in the chicken", Vet. Res, 33, pp.109~125 (2002)
- Adams LG, "Natural resistance against brucellosis: a review", Open Vet. Sci. J, 4, pp.61~71 (2010)
- Chen P, "Thermal adaptation of viruses and bacteria", Biophys. J, 98, pp.1109~1118 (2010)
- "The avian immune system. VM74" (1991)[URL]
- Gray D, "A review of the physiology of fever in birds", J. Comp. Physiol. B, 183, pp.297~312 (2013)
- Qureshi N, "The proteasome as a lipopolysaccharide-binding protein in macrophages: differential effects of proteasome inhibition on lipopolysaccharide-induced signaling events", J. Immunol, 171, pp.1515~1525 (2003)
- Cullum A, "Evolutionary adaptation to temperature. IX. Preadaptation to novel stressful environments of Escherichia coli adapted to high temperature", Evolution, 55, pp.2194~2202 (2001)
- Travisano M, "Long term experimental evolution in E. coli. IV. Targets of selection and specificity of adaptation", Genetics, 143, pp.15~26 (1996)
- Beug H, "Chicken hematopoietic cells transformed by seven strains of defective avian leukemia viruses display three distinct phenotypes of differentiation", Cell, 18, pp.375~390 (1979)
- Lee JJ, "Phellinus baumii extract influences pathogenesis of Brucella abortus by disrupting the phagocytic and intracellular trafficking pathway", J. Appl. Microbiol, 114, pp.329~338 (2013)
- Higa F, "Simplified quantitative assay system for measuring activities of drugs against intracellular Legionella pneumophila", J. Clin. Microbiol, 36, pp.1392~1398 (1998)
- Lee JJ, "Toll-like receptor 4-linked Janus kinase 2 signaling contributes to internalization of Brucella abortus by macrophages", Infect. Immun, 81, pp.2448~2458 (2013)
- Braukmann M, "Immune reaction and survivability of Salmonella Typhimurium and Salmonella Infantis i s after infection of p rimary a vian m acroph ages", PLoS One, 10, pp.e0122540~ (2015)
- Hong YH, "Analysis of chicken cytokine and chemokine gene expression following Eimeria acervulina and Eimeria tenella infections", Vet. Immunol. Immunopathol, 114, pp.209~223 (2006)
- Kapczynski D, "Characterization of cytokine expression induced by avian influenza virus infection with real-time RT-PCR", Methods Mol. Biol, 1161, pp.217~233 (2014)
- Livak KJ, "Analysis of relative gene expression data u sing r eal-time q uantitative PCR and the 2-ΔΔCt method", Methods, 25, pp.402~408 (2001)
- Adamu NB, "Serological survey of brucellosis in slaughtered local chickens, guinea fowls, ducks and turkey in North-Eastern Nigeria", Int. J. Poult. Sci, 13, pp.340~342 (2014)
- Detilleux DG, "Pathogenesis of Brucella abortus in chicken embryos", Vet. Pathol, 25, pp.138~146 (1997)
- Abbas B, "Isolation, identification and biotyping of Brucella spp. from milk product of Basrah Province", Bas. J. Vet. Res, 9, pp.152~162 (2010)
- Zeldovich KB, "Protein stability imposes limits on organism complexicity and speed of molecular evolution", Proc. Natl. Acad. Sci. USA, 104, pp.16152~16157 (2007)
- Dyson HJ, "Intrinsically unstructured proteins and their functions", Nat. Rev. Mol. Cell Biol, 6, pp.197~208 (2005)
- Uversky VN., "Natively unfolded proteins: a point where biology waits for physics", Protein Sci, 11, pp.739~756 (2002)
- Bloom JD, "Thermodynamics of neutral protein evolution", Genetics, 175, pp.255~266 (2007)
- Taverna DM, "Why are proteins marginally stable?", Proteins, 46, pp.105~109 (2002)
- Leuenberger P, "Cell-wide analysis of protein thermal unfolding reveals determinants of thermostability", Science, 355 (2017)
- Kim W, "Downregulation of chicken interleukin-17 receptor A during Eimeria infection", Infect. Immun, 82, pp.3845~3854 (2014)
- Reyes AW, "Dextran sulfate sodium upregulates MAPK signaling for the uptake and subsequent intracellular survival of Brucella abortus in murine macrophages", Microb. Pathog, 91, pp.68~73 (2016)