본문 바로가기
KERD
논문KCI 등재

Hypertonic Saline Induces Host Protective Immune Responses against Brucella abortus Infection in Mice

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류생물학

초록

Hypertonic saline (HTS) resuscitation can enhance immune responses against various pathogens, however, the effect of HTS on brucellosis is yet to be defined. In this study, we found that HTS inhibited Brucella infection in mice by augmenting Th1 immunity. HTS treatment enhanced the serum cytokines production and the expression of nitric oxide synthase (NOS2) and nuclear factor kappa B (NF-ĸB) p50 and p65, crucial anti-Brucella effectors in splenocytes. In addition, HTS treatment also inhibited the phosphorylation of MAPK signaling, accompanied by the down-regulation of the autophagy marker LC3B-II. Due to directing an appropriate immune response, HTS treatment substantially decreased bacterial burden in spleen and liver tissues. In summary, corroborating previous studies showing the antimicrobial effects of HTS, our findings indicate that HTS treatment triggers a protective immune response against Brucella infection. Additionally, these results provide promising evidence of the immunomodulatory role of HTS in controlling bacterial infections.

Abstract

Hypertonic saline (HTS) resuscitation can enhance immune responses against various pathogens, however, the effect of HTS on brucellosis is yet to be defined. In this study, we found that HTS inhibited Brucella infection in mice by augmenting Th1 immunity. HTS treatment enhanced the serum cytokines production and the expression of nitric oxide synthase (NOS2) and nuclear factor kappa B (NF-ĸB) p50 and p65, crucial anti-Brucella effectors in splenocytes. In addition, HTS treatment also inhibited the phosphorylation of MAPK signaling, accompanied by the down-regulation of the autophagy marker LC3B-II. Due to directing an appropriate immune response, HTS treatment substantially decreased bacterial burden in spleen and liver tissues. In summary, corroborating previous studies showing the antimicrobial effects of HTS, our findings indicate that HTS treatment triggers a protective immune response against Brucella infection. Additionally, these results provide promising evidence of the immunomodulatory role of HTS in controlling bacterial infections.

참고문헌 (48)

  1. Occhialini A, "The retrospective on atypical Brucella species leads to novel definitions", Microorganisms, 10, pp.813~ (2022)
  2. Laine CG, "Global estimate of human brucellosis incidence", Emerg. Infect. Dis, 29, pp.1789~ (2023)
  3. Bosilkovski M, "The current therapeutical strategies in human brucellosis", Infection, 49, pp.823~832 (2021)
  4. Carvalho TF, "Meta-analysis and advancement of brucellosis vaccinology", PLoS One, 11, pp.e0166582~ (2016)
  5. Mirzaei R, "Immunometabolism in human brucellosis : an emerging field of investigation", Microb. Pathog, 158, pp.105115~ (2021)
  6. Pfortmueller CA, "Hypertonic saline in critical illness-a systematic review", J. Crit. Care, 42, pp.168~177 (2017)
  7. Kølsen-Petersen J, "Immune effect of hypertonic saline : fact or fiction?", Acta. Anaesthesiol. Scand, 48, pp.667~678 (2004)
  8. Oliveira RP, "Clinical review : hypertonic saline resuscitation in sepsis", Crit. Care, 6, pp.1~6 (2002)
  9. Coimbra R, "Hypertonic saline resuscitation decreases susceptibility to sepsis after hemorrhagic shock", J. Trauma. Acute. Care Surg, 42, pp.602~607 (1997)
  10. Mitra S, "Hypertonic saline attenuates the cytokineinduced pro-inflammatory signature in primary human lung epithelia", PLoS. One, 12, pp.e0189536~ (2017)
  11. Coelho AMM, "Mechanisms of the beneficial effect of hypertonic saline solution in acute pancreatitis", Shock, 34, pp.502~507 (2010)
  12. Sposito F, "Hypertonic saline induces inflammation in human macrophages through the NLRP1 inflammasome", Genes. Immun, 24, pp.263~269 (2023)
  13. Shields CJ, "Hypertonic saline enhances host response to bacterial challenge by augmenting receptor-independent neutrophil intracellular superoxide formation", Ann. Surg, 238, pp.249~257 (2003)
  14. Chen L-W, "Hypertonic saline enhances host defense to bacterial challenge by augmenting toll-like receptors", Crit. Care. Med, 34, pp.1758~1768 (2006)
  15. Zhang W-C, "Elevated sodium chloride drives type I interferon signaling in macrophages and increases antiviral resistance", J. Biol. Chem, 293, pp.1030~1039 (2018)
  16. Michon A-L, "Advances toward the elucidation of hypertonic saline effects on Pseudomonas aeruginosa from cystic fibrosis patients", PLoS One, 9, pp.e90164~ (2014)
  17. Degos C, "Brucella CβG induces a dual pro-and anti-inflammatory response leading to a transient neutrophil recruitment", Virulence, 6, pp.19~28 (2015)
  18. Akbulut HH, "Determination of intracellular cytokines produced by Th1 and Th2 cells using flow cytometry in patients with brucellosis", FEMS. Immunol. Med. Microbiol, 45, pp.253~258 (2005)
  19. Elfaki MG, "Host response to Brucella infection : review and future perspective", J. Infect. Dev. Ctries, 9, pp.697~701 (2015)
  20. Lopez-Santiago R, "Immune response to mucosal Brucella infection", Front. Immunol, 10, pp.1759~ (2019)
  21. Rossetti CA, "Systems biology analysis of Brucella infected Peyer's patch reveals rapid invasion with modest transient perturbations of the host transcriptome", PLoS One, 8, pp.e81719~ (2013)
  22. Dorneles EM, "Immune response triggered by Brucella abortus following infection or vaccination", Vaccine, 33, pp.3659~3666 (2015)
  23. Martirosyan A, "An evolutionary strategy for a stealthy intracellular Brucella pathogen", Immunol. Rev, 240, pp.211~234 (2011)
  24. Priyanka, "Cytokines in brucellosis : biological rhythm at the interface of innate and adaptive immunity", Biol. Rhythm. Res, 52, pp.1031~1043 (2021)
  25. Baldwin CL, "Host immune responses to the intracellular bacteria Brucella : does the bacteria instruct the host to facilitate chronic infection?", Crit. Rev. Immunol, 26, pp.407~442 (2006)
  26. Hop HT, "Activation of NF-ĸB-mediated TNF-induced antimicrobial immunity is required for the efficient Brucella abortus clearance in RAW 264.7 cells", Front. Cell. Infect. Microbiol, 7, pp.437~ (2017)
  27. Hop HT, "Interleukin 6 promotes Brucella abortus clearance by controlling bactericidal activity of macrophages and CD8+ T cell differentiation", Infect. Immun, 87 (2019)[DOI]
  28. Hiz P, "Roles of novel IL-1 family(IL-36, IL-37, and IL-38)members in chronic brucellosis", Cytokine, 135, pp.155211~ (2020)
  29. Ip WE, "Macrophages monitor tissue osmolarity and induce inflammatory response through NLRP3 and NLRC4inflammasome activation", Nat. Commun, 6, pp.6931~ (2015)
  30. Sakata F, "Sodium chloride promotes tissue inflammation via osmotic stimuli in subtotal-nephrectomized mice", Lab. Invest, 97, pp.432~446 (2017)
  31. Oreopoulos GD, "Synergistic induction of IL-10 by hypertonic saline solution and lipopolysaccharides in murine peritoneal macrophages", Surgery, 130, pp.157~165 (2001)
  32. Xavier MN, "CD4+ T cell-derived IL-10 promotes Brucella abortus persistence via modulation of macrophage function", PLoS Pathog, 9, pp.e1003454~ (2013)
  33. Jantsch J, "Cutaneous Na+ storage strengthens the antimicrobial barrier function of the skin and boosts macrophage-driven host defense", Cell. Metab, 21, pp.493~501 (2015)
  34. Martirosyan A, "Brucella evasion of adaptive immunity", Future. Microbiol, 8, pp.147~154 (2013)
  35. Golding B, "Immunity and protection against Brucella abortus", Microbes. Infect, 3, pp.43~48 (2001)
  36. Barrionuevo P, "Brucella abortus induces intracellular retention of MHC-I molecules in human macrophages down-modulating cytotoxic CD8+ T cell responses", Cell. Microbiol, 15, pp.487~502 (2013)
  37. Guimarães G, "Immunoproteasome subunits are required for CD8+ T cell function and host resistance to Brucella abortus infection in mice", Infect. Immun, 86, pp.e00615-17~ (2018)
  38. Starr T, "Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle", Cell. Host. Microbe, 11, pp.33~45 (2012)
  39. Guo F, "Role of autophagy in inhibition of Brucella-containing vacuole-lysosome fusion in murine macrophages", Afr. J. Microbiol. Res, 5, pp.3778~3783 (2011)
  40. McCarthy CG, "Ketone body β-hydroxybutyrate is an autophagy-dependent vasodilator", JCI. Insight, 6, pp.e149037~ (2021)
  41. Forte M, "Pharmacological restoration of autophagy reduces hypertension-related stroke occurrence", Autophagy, 16, pp.1468~1481 (2020)
  42. Corcelle E, "Control of the autophagy maturation step by the MAPK ERK and p38 : lessons from environmental carcinogens", Autophagy, 3, pp.57~59 (2007)
  43. Kim K-Y, "Inhibition of autophagy promotes salinomycin-induced apoptosis via reactive oxygen species-mediated PI3K", AKT, p38 MAPK-dependent signaling in human prostate cancer cells, pp.Int. J. Mol. Sci, 2017[DOI]
  44. Deree J, "The effects of a novel resuscitation strategy combining pentoxifylline and hypertonic saline on neutrophil MAPK signaling", Surgery, 142, pp.276~283 (2007)
  45. Cervantes-Flores M, "NFκB is differentially activated in macrophages from J774A. 1 cell line infected with vaccine or virulent strains of Brucella abortus", Afr. J. Microbiol. Res, 5, pp.4227~4231 (2011)
  46. Zhao T, "Brucella abortus modulates macrophage polarization and inflammatory response by targeting glutaminases through the NF-κB signaling pathway", Front. Immunol, 14, pp.1180837~ (2023)
  47. Caamano J, "NF-κB family of transcription factors : central regulators of innate and adaptive immune functions", Clin. Microbiol. Rev, 15, pp.414~429 (2002)
  48. Zhang D, "High-salt enhances the inflammatory response by retina pigment epithelium cells following lipopolysaccharide stimulation", Mediators. Inflamm, 2015, pp.197512~ (2015)

관련 논문AI추천

The Modulatory Effect of Sodium Propionate Treatment in the Expression of Inflammatory Cytokines and Intracellular Growth of Brucella abortus 544 in Raw 264.7 Cells김석, 민원기, 이후장, Hur Jin, Huy Tran Xuan Ngoc, Kim Heejin, Nguyen Trang Thi, Reyes Alisha Wehdnesday Bernardo한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2023 · 제33권 제8호Impaired bacterial proliferation, and compromised immune response in diabetic db/db mice infected with Brucella abortus김석, 김대근, 민원기, 이진주, 이후장, 장홍희, Alisha Wehdnesday Bernardo Reyes, Hannah Leah Simborio농업생명과학연구원 · 농업생명과학연구2013 · 제47권 제2호Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice김석, 민원기, 이존화, 이후장, 장홍희, Alisha Wehdnesday Bernardo Reyes, Son Hai Vu, TRAN XUAN NGOC HUY한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2020 · 제30권 제5호Evaluation of Th1/Th2-Related Immune Response against Recombinant Proteins of Brucella abortus Infection in Mice정명환, HAN-SANG YOO, Suk KIM, Woo Bin Park, Young Bin Im한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제6호Immunization of Mice with Recombinant Brucella abortus Organic Hydroperoxide Resistance (Ohr) Protein Protects Against a Virulent Brucella abortus 544 Infection김석, 민원기, 이진주, 이후장, 장홍희, Alisha Wehdnesday Bernardo Reyes, Hannah Leah Tadeja Simborio, Huynh Tan Hop, Lauren Togonon Arayan한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제1호Immune Modulation of Recombinant OmpA against Brucella abortus 544 Infection in MiceAlisha Wehdnesday Bernardo Reyes, Hannah Leah Tadeja Simborio, Hong-hee Chang, Hu Jang Lee, Huynh Tan Hop, Jin Ju Lee, Lauren Togonon Arayan, Suk KIM, Wongi Min한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2016 · 제26권 제3호

이 간행물의 다른 논문 (24건)