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Purification, Characterization, and Cloning of a Cold-Adapted Protease from Antarctic Janthinobacterium lividum

김수미|최종일|김현도
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2018|Journal of Microbiology and Biotechnology|제28권 제3호|pp. 448-453

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키워드

expressionpurificationJanthinobacterium lividumCold-adapted protease
주제분류생물학

초록

In this study, a 107 kDa protease from psychrophilic Janthinobacterium lividum PAMC 26541 was purified by anion-exchange chromatography. The specific activity of the purified protease was 264 U/mg, and the overall yield was 12.5%. The J. lividum PAMC 25641 protease showed optimal activity at pH 7.0–7.5 and 40oC. Protease activity was inhibited by PMSF, but not by DTT. On the basis of the N-terminal sequence of the purified protease, the gene encoding the cold-adapted protease from J. lividum PAMC 25641 was cloned into the pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3) as an intracellular soluble protein.

Abstract

In this study, a 107 kDa protease from psychrophilic Janthinobacterium lividum PAMC 26541 was purified by anion-exchange chromatography. The specific activity of the purified protease was 264 U/mg, and the overall yield was 12.5%. The J. lividum PAMC 25641 protease showed optimal activity at pH 7.0–7.5 and 40oC. Protease activity was inhibited by PMSF, but not by DTT. On the basis of the N-terminal sequence of the purified protease, the gene encoding the cold-adapted protease from J. lividum PAMC 25641 was cloned into the pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3) as an intracellular soluble protein.

참고문헌 (21)

  1. Margesin R, "Biotechnological applications of psychrophiles", Environ. Technol., 31, pp.835~844 (2010)[DOI]
  2. Javed A, "Psychrophilic microbial enzymes implications in coming biotechnological processes", Am. Sci. Res. J. Eng. Technol. Sci., 23, pp.103~120 (2016)
  3. Mienda B S, "An overview of microbial proteases for industrial applications", Res. J. Pharm. Biol. Chem. Sci., 5, pp.388~396 (2014)
  4. Vazquez SC, "Extracellular proteases from eight psychrotolerant Antarctic strains", Microbiol. Res., 159, pp.157~166 (2004)[DOI]
  5. Joshi S, "Biotechnology of cold-active proteases", Biology, 2, pp.755~783 (2013)[DOI]
  6. Zhang H, "Gene cloning, expression and characterization of a novel cold-adapted protease from Planococcus sp.", J. Mol. Catal. B Enzym., 130, pp.1~8 (2016)[DOI]
  7. Vazquez S, "Properties of extracellular proteases from three psychrotolerant Stenotrophomonas maltophilia isolated from Antarctic soil", Polar Biol., 28, pp.319~325 (2005)[DOI]
  8. Zhu HY, "Purification and characterization of the cold-active alkaline protease from marine cold-adaptive Penicillium chrysogenum FS010", Mol. Biol. Rep., 36, pp.2169~2174 (2009)[DOI]
  9. Oh KH, "Isolation of a psychrotrophic Azospirillum sp. and characterization of its extracellular protease", FEMS Microbiol. Lett., 174, pp.173~178 (1999)
  10. Bradford MM, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal. Biochem., 72, pp.248~254 (1976)[DOI]
  11. Gradisar H, "Similarities and specificities of fungal keratinolytic proteases: comparison of keratinases of Paecilomyces marquandii and Doratomyces microsporus to some known proteases", Appl. Environ. Microbiol., 71, pp.3420~3426 (2005)[DOI]
  12. Laemmli UK, "Cleavage of structural proteins during the assembly of the head of bacteriophage T4", Nature, 227, pp.680~685 (1970)[DOI]
  13. 김현도, "극지 미생물들의 배양온도에 따른 성장률 및 protease activity 영향 연구", 한국미생물·생명공학회지, 42(3), pp.293~296 (2014), 한국미생물·생명공학회[DOI]
  14. Zhang H, "Neutral protease expression and optimized conditions for the degradation of blood cells using recombinant Pichia pastoris", Int. Biodeterior. Biodegradation, 93, pp.235~240 (2014)[DOI]
  15. Kobayashi T, "Extremely high alkaline protease from a deep-subsurface bacterium, Alkaliphilus transvaalensis", Appl. Microbiol. Biotechnol., 75, pp.71~80 (2007)[DOI]
  16. Kasana RC, "Isolation of a psychrotrophic Exiguobacterium sp. SKPB5 (MTCC 7803) and characterization of its alkaline protease", Curr. Microbiol., 54, pp.224~229 (2007)[DOI]
  17. Johnvesly B, "Studies on production of thermostable alkaline protease from thermophilic and alkaliphilic Bacillus sp. JB-99 in a chemically defined medium", Process Biochem., 37, pp.139~144 (2001)
  18. Wang QF, "Purification and properties of an extracellular cold-active protease from the psychrophilic bacterium Pseudoalteromonas sp. NJ276", Biochem. Eng. J., 38, pp.362~368 (2008)[DOI]
  19. Yadav SK, "Purification, biochemical characterization and performance evaluation of an alkaline serine protease from Aspergillus flavus MTCC 9952 mutant", Biocatal. Agric. Biotechnol., 4, pp.667~677 (2015)
  20. Li F, "Purification and characterization of a novel extracellular alkaline protease from Cellulomonas bogoriensis", Protein Express. Purif., 121, pp.125~132 (2016)[DOI]
  21. Mechri S, "Characterization of a novel protease from Aeribacillus pallidus strain VP3 with potential biotechnological interest", Int. J. Biol. Macromol., 94, pp.221~232 (2017)[DOI]

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