Anti-Obesity Effects of a Mixture of Fermented Ginseng, Bifidobacterium longum BORI, and Lactobacillus paracasei CH88 in High-Fat Diet-Fed Mice
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Ginseng and probiotics have anti-obesity effects in mice fed a high-fat diet (HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, a mixture of fermented ginseng and two probiotics, Bifidobacterium longum BORI and Lactobacillus paracasei CH88, was administered to HFD-fed mice for 9 weeks. The mixture significantly suppressed weight gain (p < 0.05, n = 8) and lipid deposition in the liver and adipose tissues as well as increased the mice’s food intake. The adipocyte size of the adipose tissue was significantly decreased in the mixture-fed group, especially when 0.5% fermented ginseng and 5 × 108/ml of the two probiotics were used (p < 0.05, n = 10). The expression of TNF-α in adipose tissue was efficiently downregulated in the mixture-fed group (p < 0.05, n = 4). The supplement also improved the mice’s fasting blood glucose levels (p < 0.05, n = 8) and total cholesterol feces excretion (p < 0.05, n = 8). The mixture of fermented ginseng and B. longum BORI and L. paracasei CH88 could have an anti-obesity effect and suppress lipid deposit in the liver and adipose tissues.
Abstract
Ginseng and probiotics have anti-obesity effects in mice fed a high-fat diet (HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, a mixture of fermented ginseng and two probiotics, Bifidobacterium longum BORI and Lactobacillus paracasei CH88, was administered to HFD-fed mice for 9 weeks. The mixture significantly suppressed weight gain (p < 0.05, n = 8) and lipid deposition in the liver and adipose tissues as well as increased the mice’s food intake. The adipocyte size of the adipose tissue was significantly decreased in the mixture-fed group, especially when 0.5% fermented ginseng and 5 × 108/ml of the two probiotics were used (p < 0.05, n = 10). The expression of TNF-α in adipose tissue was efficiently downregulated in the mixture-fed group (p < 0.05, n = 4). The supplement also improved the mice’s fasting blood glucose levels (p < 0.05, n = 8) and total cholesterol feces excretion (p < 0.05, n = 8). The mixture of fermented ginseng and B. longum BORI and L. paracasei CH88 could have an anti-obesity effect and suppress lipid deposit in the liver and adipose tissues.
참고문헌 (38)
- Jung S, "Anti-obesity and anti-inflammatory effects of high hydrostatic pressure extracts of ginseng in high-fat diet induced obese rats", J. Funct. Foods, 10, pp.169~177 (2014)[DOI]
- Ku S, "Review on Bifidobacterium bifidum BGN4: functionality and nutraceutical applications as a probiotic microorganism", Int. J. Mol. Sci., 17, pp.E1544~ (2016)[DOI]
- Shen J, "The gut microbiota, obesity and insulin resistance", Mol. Aspects Med., 34, pp.39~58 (2012)
- Park MS, "The efficacy of Bifidobacterium longum BORI and Lactobacillus acidophilus ad031 probiotic treatment in infants with rotavirus infection", Nutrients, 9, pp.E887~ (2017)[DOI]
- Li Z, "Anti-obese effects of two lactobacilli and two bifidobacteria on ICR mice fed on a high fat diet", Biochem. Biophys. Res. Commun., 480, pp.222~227 (2016)[DOI]
- Ku S, "Finding and producing probiotic glycosylases for the biocatalysis of ginsenosides: a mini review", Molecules, 21, pp.645~ (2016)[DOI]
- Seockmo Ku, "Whole-Cell Biocatalysis for Producing Ginsenoside Rd from Rb1 Using Lactobacillus rhamnosus GG", Journal of Microbiology and Biotechnology, 26(7), pp.1206~1215 (2016), 한국미생물·생명공학회[DOI]
- 구석모, "Effects of ascorbic acid on a-L-arabinofuranosidase and a-Larabinopyranosidase activities from Bifidobacterium longum RD47 and its application to whole cell bioconversion of ginsenoside", Applied Biological Chemistry, 58(6), pp.857~865 (2015), 한국응용생명화학회
- Karikura M, "Studies on absorption, distribution, excretion and metabolism of ginseng saponins. V. The decomposition products of ginsenoside Rb2 in the large intestine of rats", Chem. Pharm. Bull., 38, pp.2859~ (1990)[DOI]
- Ouwehand AC, "Probiotics: mechanisms and established effects", Int. Dairy J., 9, pp.43~52 (1999)[DOI]
- Folch J, "A simple method for the isolation and purification of total lipides from animal tissues", J. Biol. Chem., 226, pp.497~ (1957)
- Thaler JP, "Inflammation and obesity pathogenesis: the hypothalamus heats up", J. Clin. Endocrinol. Metab., 95, pp.4077~ (2010)
- Liu W, "Saponins (ginsenosides) from stems and leaves of Panax quinquefolium prevented high-fat diet-induced obesity in mice", Phytomedicine, 15, pp.1140~1145 (2008)[DOI]
- Yoo SR, "Probiotics L. plantarum and L. curvatus in combination alter hepatic lipid metabolism and suppress diet-induced obesity", Obesity, 21, pp.2571~2578 (2013)[DOI]
- Se Na Yun, "Wild Ginseng Prevents the Onset of High-Fat Diet Induced Hyperglycemia and Obesity in ICR Mice", Archives of Pharmacal Research, 27(7), pp.790~796 (2004), 대한약학회
- Zhang X-L, "The protective effects of probiotic-fermented soymilk on high-fat diet-induced hyperlipidemia and liver injury", J. Funct. Fo, 30, pp.220~227 (2017)[DOI]
- Sakakibara K, "Effect of ginseng saponins on cholesterol metabolism. I. The level and the synthesis of serum and liver cholesterol in rats treated with ginsenosides", Chem. Pharm. Bull., 23, pp.1009~ (1975)[DOI]
- Lye HS, "Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol", J. Dairy Sci., 93, pp.1383~1392 (2010)[DOI]
- Andersson U, "Probiotics lower plasma glucose in the high-fat fed C57BL", 6J mouse, 2010, pp.1, 189~196
- Park S, "Ginsenosides Rb1 and Rg1 suppress triglyceride accumulation in 3T3-L1 adipocytes and enhance <TEX>${\beta}$<", TEX>-cell insulin secretion and viability in Min6 cells via PKA-dependent pathways, 2008, pp.72, 2815~2823
- Hwang JT, "Biological active components found in Panax ginseng improve glucose uptake via AMPK signaling pathway", FASEB J., 22, pp.683~ (2008)
- Dowman JK, "Pathogenesis of non-alcoholic fatty liver disease", QJM, 103, pp.71~83 (2010)[DOI]
- Zhang L, "Ginseng and obesity: observations and understanding in cultured cells, animals and humans", J. Nutr. Biochem., 44, pp.1~10 (2017)[DOI]
- Zhang Y, "Protopanaxatriol, a novel PPAR<TEX>${\gamma}$<", TEX> antagonist from Panax ginseng, alleviates steatosis in mice, 2014, pp.4, 7375~
- Wu C-C, "Effect of strain K21 on high-fat diet-fed obese mice", Evid. Based Complement. Alternat. Med., 2015, pp.391767~ (2015)
- PARK, YOO HEON, "Effect of Dietary Inclusion of Lactobacillus acidophilus ATCC 43121 onCholesterol Metabolism in Rats", Journal of Microbiology and Biotechnology, 17(4), pp.655~662 (2007), 한국미생물·생명공학회
- Dentin R, "Carbohydrate responsive element binding protein (ChREBP) and sterol regulatory element binding protein-1c (SREBP-1c): two key regulators of glucose metabolism and lipid synthesis in liver", Biochimie, 87, pp.81~86 (2005)[DOI]
- Crandall DL, "Adipocyte blood flow: influence of age, anatomic location, and dietary manipulation", Am. J. Physiol., 247, pp.R46~R51 (1984)
- Murano I, "Dead adipocytes, detected as crown-like structures, are prevalent in visceral fat depots of genetically obese mice", J. Lipid Res., 49, pp.1562~ (2008)[DOI]
- Strissel KJ, "Adipocyte death, adipose tissue remodeling, and obesity complications", Diabetes, 56, pp.2910~ (2007)[DOI]
- Lee H, "Korean red ginseng (Panax ginseng) prevents obesity by inhibiting angiogenesis in high fat diet-induced obese C57BL", 6J mice, 2013, pp.53, 402~408
- Takemura N, "Lactobacillus plantarum strain no. 14 reduces adipocyte size in mice fed high-fat diet", Exp. Biol. Med., 235, pp.849~856 (2010)[DOI]
- 김성옥, "지방세포 3T3-L1에 인삼 사포닌 Re와 의이인 추출액 처리시 비만관련 유전자인 TNF-α, lipoprotein lipase, leptin 및 resistin 발현 조절에 미치는 영향", 생명과학회지, 17(11), pp.1523~1532 (2007), 한국생명과학회
- Oh J, "Ginseng and its active components ginsenosides inhibit adipogenesis in 3T3-L1 cells by regulating MMP-2 and MMP-9", Evid. Based Complement. Alternat. Med., 2012, pp.265023~ (2012)
- Ciaraldi TP, "Regulation of glucose transport and insulin signaling by troglitazone or metformin in adipose tissue of type 2 diabetic subjects", Diabetes, 51, pp.30~36 (2002)[DOI]
- 최선실, "Revisiting PPARγ as a target for the treatment of metabolic disorders", BMB Reports, 47(11), pp.599~608 (2014), 생화학분자생물학회
- Masuno H, "Ginsenosides increase secretion of lipoprotein lipase by 3T3-L1 adipocytes", Biosci. Biotechnol. Biochem., 60, pp.1962~1965 (1996)[DOI]
- Sekiya K, "Enhancement of adipose differentiation of mouse 3T3-L1 fibroblasts by ginsenosides", Phytother. Res., 1, pp.58~60 (1987)[DOI]