본문 바로가기
KERD
논문KCI 등재

Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Pantoea sp. 5-Enolpyruvylshikimate-3- Phosphate Synthase

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류생물학

초록

Glyphosate inhibits the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway. A mutant of EPSPS from Pantoea sp. was identified using sitedirected mutagenesis. The mutant showed significantly improved glyphosate resistance. The mutant had mutations in three amino acids: Gly97 to Ala, Thr 98 to Ile, and Pro 102 to Ser. These mutation sites in Escherichia coli have been studied as significant active sites of glyphosate resistance. However, in our research, they were found to jointly contribute to the improvement of glyphosate tolerance. In addition, the level of glyphosate tolerance in transgenic Arabidopsis confirmed the potentiality of the mutant in breeding glyphosateresistant plants.

Abstract

Glyphosate inhibits the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway. A mutant of EPSPS from Pantoea sp. was identified using sitedirected mutagenesis. The mutant showed significantly improved glyphosate resistance. The mutant had mutations in three amino acids: Gly97 to Ala, Thr 98 to Ile, and Pro 102 to Ser. These mutation sites in Escherichia coli have been studied as significant active sites of glyphosate resistance. However, in our research, they were found to jointly contribute to the improvement of glyphosate tolerance. In addition, the level of glyphosate tolerance in transgenic Arabidopsis confirmed the potentiality of the mutant in breeding glyphosateresistant plants.

참고문헌 (31)

  1. Zobiole L, "Glyphosate effects on photosynthesis, nutrient accumulation, and nodulation in glyphosate-resistant soybean", J. Plant Nutr. Soil Sci., 175, pp.319~330 (2012)[DOI]
  2. Baerson SR, "Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase", Plant Physiol., 129, pp.1265~1275 (2002)[DOI]
  3. Yi SY, "A novel naturally occurring class I 5-enolpyruvylshikimate-3-phosphate synthase from Janibacter sp. confers high glyphosate tolerance to rice", Sci. Rep., 6, pp.19104~ (2016)[DOI]
  4. Cao G, "A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants", PLoS One, 7, pp.e38718~ (2012)[DOI]
  5. Priestman MA, "5-Enolpyruvylshikimate-3-phosphate synthase from Staphylococcus aureus is insensitive to glyphosate", FEBS Lett., 579, pp.728~732 (2005)[DOI]
  6. Tian YS, "Isolation from Proteus mirabilis of a novel class I 5- enopyruvylshikimate-3-phosphate synthase with glyphosate tolerance in transgenic Arabidopsis thaliana", Acta Physiol. Plant, 36, pp.549~554 (2014)[DOI]
  7. Liang A, "A single residue mutation of 5-enoylpyruvylshikimate-3-phosphate synthase in Pseudomonas stutzeri enhances resistance to the herbicide glyphosate", Biotechnol. Lett., 30, pp.1397~1401 (2008)[DOI]
  8. Li H, "DNA shuffling approach for recombinant polyvalent OmpAs against V. alginolyticus and E. tarda infections", Fish Shellfish Immunol., 58, pp.508~513 (2016)[DOI]
  9. Li J, "The Arabidopsis thaliana trp5 mutant has a feedback-resistant anthranilate synthase and elevated soluble tryptophan", Plant Physiol., 110, pp.51~59 (1996)[DOI]
  10. Locher CP, "Development of novel vaccines using DNA shuffling and screening strategies", Curr. Opin. Mol. Ther., 6, pp.34~39 (2004)
  11. Sakaue R, "Thermostabilization of bacterial fructosyl-amino acid oxidase by directed evolution", Appl. Environ. Microbiol., 69, pp.139~145 (2003)[DOI]
  12. Stalker DM, "A single amino acid substitution in the enzyme 5-enolpyruvylshikimate-3-phosphate synthase confers resistance to the herbicide glyphosate", J. Biol. Chem., 260, pp.4724~4728 (1985)
  13. Healy-Fried ML, "Structural basis of glyphosate tolerance resulting from mutations of Pro101 in Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthase", J. Biol. Chem., 282, pp.32949~32955 (2007)[DOI]
  14. He M, "A new type of class I bacterial 5-enolpyruvylshikimate-3-phosphate synthase mutants with enhanced tolerance to glyphosate", Biochim. Biophys. Acta, 1568, pp.1~6 (2001)[DOI]
  15. Tian YS, "Mutation by DNA shuffling of 5-enolpyruvylshikimate-3-phosphate synthase from Malus domestica for improved glyphosate resistance", Plant Biotechnol. J., 11, pp.829~838 (2013)[DOI]
  16. Tian YS, "Improvement of glyphosate resistance through concurrent mutations in three amino acids of the Ochrobactrum 5- enolpyruvylshikimate-3-phosphate synthase", Appl. Environ. Microbiol., 77, pp.8409~8414 (2011)[DOI]
  17. Wang HY, "Expression of a bacterial aroA mutant, aroA-M1, encoding 5-enolpyruvylshikimate-3-phosphate synthase for the production of glyphosateresistant tobacco plants", J. Plant Res., 116, pp.455~460 (2003)[DOI]
  18. Della-Cioppa G, "Targeting a herbicide-resistant enzyme from Escherichia coli to chloroplasts of higher plants", Nat. Biotechnol., 5, pp.579~584 (1987)[DOI]
  19. Funke T, "Structural basis of glyphosate resistance resulting from the double mutation Thr97<TEX>$\rightarrow$<", TEX>Ile and Pro101<TEX>$\rightarrow$<, J. Biol. Chem., pp.2009, 284[DOI]
  20. He M, "A T42M substitution in bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) generates enzymes with increased resistance to glyphosate", Biosci. Biotechnol. Biochem., 67, pp.1405~1409 (2003)[DOI]
  21. Kahrizi D, "Simultaneous substitution of Gly96 to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phosphate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate", Plant Cell Rep., 26, pp.95~104 (2007)
  22. de Almeida Lopes KB, "Culturable endophytic bacterial communities associated with field-grown soybean", J. Appl. Microbiol., 120, pp.740~755 (2016)[DOI]
  23. Liu F, "Cloning and characterization of 5-enolpyruvylshikimate-3-phosphate synthase from Pantoea sp", Genet. Mol. Res., 14, pp.19233~19241 (2015)[DOI]
  24. Tate T, "Effect of glyphosate on the development of Pseudosuccinea columella snails", Arch. Environ. Contam. Toxicol., 33, pp.286~289 (1997)[DOI]
  25. Thomas WE, "Glyphosate negatively affects pollen viability but not pollination and seed set in glyphosate-resistant corn", Weed Sci., 52, pp.725~734 (2004)[DOI]
  26. Janel LH, "The EPSPS Pro106Ser substitution solely accounts for glyphosate resistance in a goosegrass (Eleusine indica) population from Tennessee, United States", J. Integr. Agric., 15, pp.1304~1312 (2016)[DOI]
  27. Sost D, "Substitution of Gly-96 to Ala in the 5-enolpyruvylshikimate 3-phosphate synthase of Klebsiella pneumoniae results in a greatly reduced affinity for the herbicide glyphosate", Arch. Biochem. Biophys., 282, pp.433~436 (1990)[DOI]
  28. Gasser CS, "Structure, expression, and evolution of the 5-enolpyruvylshikimate-3-phosphate synthase genes of petunia and tomato", J. Biol. Chem., 263, pp.4280~4287 (1988)
  29. Stauffer ME, "Chemical shift mapping of shikimate-3-phosphate binding to the isolated N-terminal domain of 5-enolpyruvylshikimate-3-phosphate synthase", FEBS Lett., 499, pp.182~186 (2001)[DOI]
  30. Jing Han, "Functional Characterization of aroA from Rhizobium leguminosarum with Significant Glyphosate Tolerance in Transgenic Arabidopsis", Journal of Microbiology and Biotechnology, 24(9), pp.1162~1169 (2014), 한국미생물·생명공학회[DOI]
  31. Peng RH, "A novel 5-enolpyruvylshikimate-3-phosphate synthase from Rahnella aquatilis with significantly reduced glyphosate sensitivity", PLoS One, 7, pp.e39579~ (2012)[DOI]

이 간행물의 다른 논문 (13건)