Effect of Rosemary Essential Oil and Trichoderma koningiopsis VOCs on Pathogenic Fungi Responsible for Ginseng Root-rot Disease
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Rosemary essential oil was evaluated for antifungal potentiality against six major ginseng pathogens: Sclerotinia sclerotiorum, Sclerotinia nivalis, Cylindrocarpon destructans, Alternaria panax, Botrytis cinerea, and Fusarium oxysporum. The in vitro fungicidal effects of two commonly used fungicides, namely mancozeb and fenhexamid, and the volatile organic compounds (VOCs) of Trichoderma koningiopsis T-403 on the mycelial growth were investigated. The results showed that rosemary essential oil is active against all of the pathogenic strains of ginseng root rot, whereas rosemary oil displayed high ability to inhibit the Sclerotinia spp. growth. The highest sensitivity was S. nivalis, with complete inhibition of growth at 0.1% v/v of rosemary oil, followed by Alternaria panax, which exhibited 100% inhibition at 0.3% v/v of the oil. Minimum inhibitory concentrations (MICs) of rosemary oil ranged from 0.1 % to 0.5 % (v/v). Chemical analysis using GC-MS showed the presence of thirty-two constituents within rosemary oil from R. officinals L. Camphore type is the most frequent sesquiterpene in rosemary oil composition. Mancozeb and fenhexamid showed their highest inhibition effect (45% and 30%, respectively) against A. panax. T. koningiopsis T-403 showed its highest inhibition effect (84%) against C. destructans isolate. This study may expedite the application of antifungal natural substances from rosemary and Trichoderma in the prevention and control of phytopathogenic strains in ginseng root infections.
Abstract
Rosemary essential oil was evaluated for antifungal potentiality against six major ginseng pathogens: Sclerotinia sclerotiorum, Sclerotinia nivalis, Cylindrocarpon destructans, Alternaria panax, Botrytis cinerea, and Fusarium oxysporum. The in vitro fungicidal effects of two commonly used fungicides, namely mancozeb and fenhexamid, and the volatile organic compounds (VOCs) of Trichoderma koningiopsis T-403 on the mycelial growth were investigated. The results showed that rosemary essential oil is active against all of the pathogenic strains of ginseng root rot, whereas rosemary oil displayed high ability to inhibit the Sclerotinia spp. growth. The highest sensitivity was S. nivalis, with complete inhibition of growth at 0.1% v/v of rosemary oil, followed by Alternaria panax, which exhibited 100% inhibition at 0.3% v/v of the oil. Minimum inhibitory concentrations (MICs) of rosemary oil ranged from 0.1 % to 0.5 % (v/v). Chemical analysis using GC-MS showed the presence of thirty-two constituents within rosemary oil from R. officinals L. Camphore type is the most frequent sesquiterpene in rosemary oil composition. Mancozeb and fenhexamid showed their highest inhibition effect (45% and 30%, respectively) against A. panax. T. koningiopsis T-403 showed its highest inhibition effect (84%) against C. destructans isolate. This study may expedite the application of antifungal natural substances from rosemary and Trichoderma in the prevention and control of phytopathogenic strains in ginseng root infections.
참고문헌 (51)
- Randall JA, "American Ginseng in Iowa: Producing IOWA STATE UNIVERSITY F-401 Forestry Extension Ames, Iowa Series on Ginseng. F -400, F401, F402, & F403", ~ (2013)
- Davis JW, "Growing and marketing ginseng, goldenseal and other woodland medicinals", ~ (2014)
- Tawaha K, "Antioxidant activity and total phenolic content of selected Jordanian plant species", Food Chem, 104, pp.1372~1378 (2007)
- Moreira MR, "Inhibitory parameters of essential oils to reduce a foodborne pathogen", LWT, 38, pp.565~570 (2005)
- Peng Y, "Determination of active components in rosemary by capillary electrophoresis with electrochemical detection", J. Pharm. Biomed. Anal, 39, pp.431~437 (2005)
- Hyldgaard M, "Essential oils in food preservation : mode of action, synergies and interactions with food matrix components", Front. Microbiol, 3, pp.12~ (2012)
- Cardile V, "Essential oils of Salvia bracteata and Salvia rubifolia from Lebanon : Chemical composition, antimicrobial activity and inhibitory effect on human melanoma cells", J. Ethnopharmacol, 126, pp.265~272 (2009)
- Oussalah M, "Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria : E. coli O157 : H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes", Food Control, 18, pp.414~420 (2007)
- Silva F, "Antifungal activity of Coriandrum sativum essential oil, its mode of action against candida species and potential synergism with amphotericin B", Phytomedicine, 19, pp.42~47 (2011)
- Schnitzler P, "Screening for antiviral activities of isolated compounds from essential oils. 253643", J. Evid. Based Complement. Alternat. Med., 25, pp.36~43 (2011)
- Bertoli A, "Hypericum species as sources of valuable essential oils", Med. Arom. Plant Sci. Biotechnol, 5, pp.29~47 (2010)
- Tian J, "The mechanism of antifungal action of essential oil from dill (Anethum graveolens L.) on Aspergillus flavus", PLoS One, 7, pp.e30147~ (2012)
- Habtemariam S, "The therapeutic potential of rosemary(Rosmarinus officinalis)diterpenes for Alzheimer’s disease", J. Evid. Based Complement. Alternat. Med. Article, 2016, pp.2680409~ (2016)
- Estévez M, "Sage and rosemary essential oils versus BHT for the inhibition of lipid oxidative reactions in liver pâté", LWT Food Sci. Technol, 40, pp.58~65 (2007)
- Valgimigli L., "Essential Oils as Natural Food Additives: Composition, Applications, Antioxidant and Antimicrobial Properties" (2012), Nova Science Publishers
- Issabeagloo E, "Antimicrobial effects of rosemary (Rosmarinus officinalis L.)essential oils against Staphylococcus spp", Afr. J. Microbiol. Res., 6, pp.5039~5042 (2012)
- Ojeda-Sana AM, "New insights into antibacterial and antioxidant activities of rosemary essential oils and their main components", Food Control, 31, pp.189~195 (2013)
- Zaouali Y, "Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities", Food Chem. Toxicol., 48, pp.3144~3152 (2010)
- Piechulla B, "The emerging importance of microbial volatile organic compounds", Plant Cell Environ, 37, pp.811~812 (2014)
- Campos VP, "Volatiles produced by interacting microorganisms potentially useful for the control of plant pathogens", Cienciae Agrotec Lavras, 34, pp.525~535 (2010)
- Cortes-Barco AM, "Comparison of induced resistance activated by benzothiadiazole, (2R, 3R)-butanediol and an isoparaffin mixture against anthracnose of Nicotiana benthamiana", Plant Pathol, 59, pp.643~653 (2010)
- Lee S, "Age matters : the effects of volatile organic compounds emitted by Trichoderma atroviride on plant growth", Arch. Microbiol, 197, pp.723~727 (2015)
- Domsch KH, "Compendium of soil fungi", pp.405~859 (1980), Academic Press
- Moubasher AH., "Soil fungi in Qatar and other Arab countries" (1993), The Centre of Scientific and Applied Research University of Qater
- Jiang L, "Comparison of disk diffusion, agar dilution, and broth microdilution for antimicrobial susceptibility testing of five chitosans", PhD, pp.2011
- Ben Kaab S, "Rosmarinus officinalis essential oil as an effective antifungal and herbicidal agent", Spanish J. Agr. Res, 17, pp.e1006~ (2019)
- Messgo-Moumene S, "Antifungal power of citrus essential oils against potato late blight causative agent", J. Essen. Oil Res, 27, pp.169~176 (2015)
- Dennis C, "Antagonistic properties of species-groups of Trichoderma: iii. hyphal interaction", Trans. Br. Mycol. Soc., 57, pp.363~369 (1971)
- "SAS", 2011
- Punja ZK, "Growth, pathogenicity and infection behaviour, and genetic diversity of Rhexocercosporidium panicis isolates from ginseng roots in British Columbia", Canadian J. Plant Pathol, 35, pp.503~513 (2008)
- 신종환, "The Effect of Fungicides on Mycelial Growth and Conidial Germination of the Ginseng Root Rot Fungus, Cylindrocarpon destructans", Mycobiology, 45(3), pp.220~225 (2017), 한국균학회
- Billard A, "Fungicides-Beneficial and Harmful Aspects", pp.61~78 (2011), InTech Publisher
- Gullino ML, "Mancozeb : past, present, and future", Plant Dis, 94, pp.1076~1087 (2010)
- Jose LHS, "Sensitivity of Venturia inaequalis chilean isolates to difenoconazole, fenarimol, mancozeb, and pyrimethanil", Chil. J. Agr. Res, 71, pp.39~44 (2011)
- Hwang E, "Postharvest treatments for the reduction of mancozeb in fresh apples", J. Agr. Food Chem., 49, pp.3127~3132 (2001)
- Buck JW, "Combinations of fungicides with phylloplane yeasts for improved control of Botrytis cinerea on geranium seedlings", Phytopathology, 94, pp.196~202 (2004)
- Walker AS, "Botrytis pseudocinerea, a new cryptic species causing grey mould in French vineyards in sympatry with Botrytis cinerea", Phytopathology, 101, pp.1433~1445 (2011)
- Szabo M, "Control plant-parasitic nematodes with Trichoderma species and nematodetrapping fungi : the role of chi18-5 and chi18-12 genes in nematode egg-parasitism", Biol. Control, 63, pp.121~128 (2012)
- Howell CR, "Trichoderma and Gliocladium", pp.173~184 (1998), Taylor and Francis
- Mathys J, "Genome-wide characterization of ISR induced in Arabidopsis thaliana by Trichoderma hamatum T382 against Botrytis cinerea infection", Front. Plant Sci, 3, pp.108~ (2012)
- Contreras-Cornejo HA, "Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis", Plant Physiol, 149, pp.1579~1592 (2009)
- Lee S, "Volatile organic compounds emitted by Trichoderma species mediate plant growth", Fungal Biol. Biotechnol, 3, pp.7~ (2016)
- Pinto E, "Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species", J. Med. Microbiol, 58, pp.1454~1462 (2009)
- Sirocchi V, "Biogenic amines as freshness index of meat wrapped in a new active packaging system formulated with essential oils of Rosmarinus officinalis", Inter. J. Food Nutr. Sci, 64, pp.921~928 (2013)
- Sánchez-González L, "Antimicrobial activity of polysaccharide films containing essential oils", Food Control, 22, pp.1302~1310 (2011)
- Atti-Santos AC, "Physico-chemical Evaluation of Rosmarinus officinalis L", Essential Oils, 48, pp.1035~1039 (2005)
- Baser KHC, "Handbook of Essential Oils : Science, Technology and Applications" (2010), CRC Press
- Bai J, "Chilling and heating may regulate C6 volatile aroma production by different mechanisms in tomato(Solanum lycopersicum)fruit", Postharvest Biol. Technol, 60, pp.111~120 (2011)
- Elaissi A, "Correlation between chemical composition and antibacterial activity of essential oils from fifteen Eucalyptus species growing in the Korbous and Jbel Abderrahman Arboreta(North East Tunisia)", Molecules, 17, pp.3044~3057 (2012)
- Fu Y, "Antimicrobial activity of clove and rosemary essential oils alone and in combination", Phytother. Res, 21, pp.989~994 (2007)
- Sienkiewicz M, "The potential of use basil and rosemary essential oils as effective antibacterial agents", Molecules, 18, pp.9334~9351 (2013)
