저장 생강 유래 부패 곰팡이 Fusarium oxysporum에 대한 식물 추출물의 항진균 활성
Antifungal activity of plant extracts against Fusarium oxysporum isolated from spoilage ginger
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
This study was aimed at evaluating the antifungal potentials of twelve plant extracts (Terminariae Fructus, Glycyrrhizae Radix, Cinnamomi Cortex, Taraxacum Platycarpum, Rubi Fructus, Caesalpiniae Lignum, Myristicae Semen, Caryophylli Flos, Sanguisorbae Radix, Alpiniae Katsumadaii Semen, Scutellariae Radix, Astragalus membranaceus) in preventing storage rot symptoms in ginger (Zingiber officinale). Samples were collected from ginger that had been stored for 120 days at 10-12℃. A total of ten fungi were isolated from stored non-spoiled and spoiled ginger and identified using internal transcribed spacer gene sequencing as Hypocrea virescentiflava, Fusarium oxysporum, Acremonium strictum, Fusarium solani, and Cadophora fastigiata. The causal fungus of storage rot was identified as F. oxysporum by pathogenicity test. Twelve candidate plant extracts were screened for antifungal activity against F. oxysporum isolated from spoiled ginger by using the disk diffusion method. In minimal inhibitory concentration (MICs) test, all ethanolic extracts of Cinnamomi Cortex, Taraxacum platycarpum, and Caryophylli Flos exhibited higher antifungal activity against F. oxysporum than other plant extracts. The MIC value of Cinnamomi Cortex and Caryophylli Flos were determined to be 10 ìg/disc. The MIC of T. platycarpum, 2.5 ìg/disc, was the lowest. Based on the results, it was concluded that Cinnamomi Cortex, T. platycarpum, and Caryophylli Flos have potential to serve as effective treatments to prevent storage rot of ginger.
Abstract
This study was aimed at evaluating the antifungal potentials of twelve plant extracts (Terminariae Fructus, Glycyrrhizae Radix, Cinnamomi Cortex, Taraxacum Platycarpum, Rubi Fructus, Caesalpiniae Lignum, Myristicae Semen, Caryophylli Flos, Sanguisorbae Radix, Alpiniae Katsumadaii Semen, Scutellariae Radix, Astragalus membranaceus) in preventing storage rot symptoms in ginger (Zingiber officinale). Samples were collected from ginger that had been stored for 120 days at 10-12℃. A total of ten fungi were isolated from stored non-spoiled and spoiled ginger and identified using internal transcribed spacer gene sequencing as Hypocrea virescentiflava, Fusarium oxysporum, Acremonium strictum, Fusarium solani, and Cadophora fastigiata. The causal fungus of storage rot was identified as F. oxysporum by pathogenicity test. Twelve candidate plant extracts were screened for antifungal activity against F. oxysporum isolated from spoiled ginger by using the disk diffusion method. In minimal inhibitory concentration (MICs) test, all ethanolic extracts of Cinnamomi Cortex, Taraxacum platycarpum, and Caryophylli Flos exhibited higher antifungal activity against F. oxysporum than other plant extracts. The MIC value of Cinnamomi Cortex and Caryophylli Flos were determined to be 10 ìg/disc. The MIC of T. platycarpum, 2.5 ìg/disc, was the lowest. Based on the results, it was concluded that Cinnamomi Cortex, T. platycarpum, and Caryophylli Flos have potential to serve as effective treatments to prevent storage rot of ginger.
참고문헌 (40)
- Cornell DW, "The pungent principle of ginger and their importance in certain ginger products", Food Technol Austral, 21, pp.570~575 (1969)
- 이현석, "반응표면분석에 의한 생강의 분자압축탈수 방법 최적화", 한국식품과학회지, 42(4), pp.398~406 (2010), 한국식품과학회
- Lee WH, "Ecology of rhizome rot incidence of ginger and relation of soil texture, chemistry and biology", Korean J Environ Agric, 17, pp.1~4 (1998)
- Kim CH, "Effect of soil disinfection, fungicide application, and narrow ridge cultivation on development of ginger rhizome rot caused by Pythium myriotylum in fields", Korean J Plant Pathol, 14, pp.253~259 (1998)
- Peterson JL, "Studies on resistance in radish to Fusarium oxysporum f. sp. conglutinans", Phytopathology, 50, pp.807~816 (1960)
- Desjardins AE, "Mycotoxins in plant pathogenesis", Mol Plant-Microbe Interact, 10, pp.147~152 (1997)[DOI]
- Van Asch MAJ, "Phytotoxicity of fumonisin B1, moniliformin, and T-2 toxin in corn callus cultures", Phytopathology, 82, pp.1330~1332 (1992)[DOI]
- 김희수, "열처리 조건에 따른 다진생강의 저장 중 품질특성", Food Science and Preservation, 17(6), pp.784~792 (2010), 한국식품저장유통학회
- Choi YH, "Improvement of quality and prolongation in chopped ginger storage", Korean J Agric Chem Biotechnol, 40, pp.123~127 (1997)
- 이명희, "생강다대기의 저장 중 품질특성", Food Science and Preservation, 17(1), pp.23~29 (2010), 한국식품저장유통학회
- 정헌식, "에틸렌 흡착제가 MA 포장 생강의 품질에 미치는 영향", 원예과학기술지, 28(1), pp.82~88 (2010), 한국원예학회
- 이혜옥, "강제 증발 가습 시스템을 이용한 생강의 저장기간 연장·", 원예과학기술지, 35(5), pp.555~567 (2017), 한국원예학회
- Paull RE, "Compositional changes in ginger rhizomes during storage", J Am Soc Hortic Sci, 113, pp.584~588 (1988)
- Paull RE, "Control of weight loss and spouting of ginger rhizome in storage", Hortscience, 23, pp.734~736 (1988)
- CHOI, GYUNG JA, "In Vivo Antifungal Activities of 67 Plant Fruit Extracts Against Six PlantPathogenic Fungi", Journal of Microbiology and Biotechnology, 16(3), pp.491~495 (2006), 한국미생물·생명공학회
- 안선미, "황련 추출물의 고추역병에 대한 In-vitro항진균 활성 및 고추 생육촉진 효과", 한국미생물·생명공학회지, 37(3), pp.280~286 (2009), 한국미생물·생명공학회
- 이진만, "정향 추출물로부터 항진균성 물질의 분리 및 동정", 한국응용과학기술학회지, 31(4), pp.740~747 (2014), 한국응용과학기술학회
- 김범수, "보리 흰가루병 곰팡이 Fusarium graminearum에 대한 산초 추출물의 항진균 활성", 생명과학회지, 18(7), pp.974~979 (2008), 한국생명과학회
- 조은미, "가자, 오배자, 계피 추출물을 이용한 화장품 제형에서의 방부효과", 대한화장품학회지, 34(4), pp.325~331 (2008), 사단법인 대한화장품학회
- CLSI, "Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; Approved standard", CLSI document M31-A3, Clinical and Laboratory Standards Institute, 13~24 (2006)
- Mishra BB, "Shelf life extension of fresh ginger (Zingiber officinale) by gamma irradiation", J food sci, 69, pp.274~279 (2004)
- Trujillo EE, "Fusarium yellows and rhizome rot of common ginger", Phytopathology, 53, pp.1370~1371 (1964)
- Dake GN, "Association of pathogens with rhizome rot of ginger in Kerala", Indian Phytopathol, 42, pp.116~119 (1989)
- Stirling AM, "Erwinia chrysanthemi, the cause of soft rot in ginger (Zingiber officinale) in Australia", Australas Plant Pathol, 31, pp.419~420 (2002)[DOI]
- McCarter SM, "Comparative pathogenicity of Pythium aphanidermatum and Pythium myriotylum to twelve plant species and intraspecific variation in virulence", Phytopathology, 60, pp.264~268 (1970)[DOI]
- Khaled H, "Temperature Effect on mycelial growth and on disease incidence of Fusarium oxysporum f. sp. radicis-lycopersici", Plant Pathol J, 5, pp.233~238 (2006)[DOI]
- 신지훈, "인삼 뿌리썩음병 발병에 미치는 토양전염성병원균과 토양환경요인", 한국토양비료학회지(Korean Journal of Soil Science and Fertilizer), 45(3), pp.370~376 (2012), 한국토양비료학회[DOI]
- Li Y, "First report of ginger rhizome rot caused by Fusarium oxysporum in China", Plant disease, 98, pp.282~ (2014)
- Jeong ET, "Antimicrobial activity and antimutagenesis of cinnamon (Cinnamomum cassia Blume) bark extract", J Food Hyg Saf, 13, pp.337~343 (1998)
- Yang L, "Total phenolic acid content, total flavonoid content and antioxidant activity of dandelion flowers", Food Sci, 32, pp.160~163 (2011)
- Xia DZ, "Phenolic compounds from the edible seeds extract of Chinese Mei (Prunus mume Sieb. Et Zucc) and their antimicrobial activity", LWT-Food Sci Technol, 44, pp.347~349 (2011)[DOI]
- Karapinar M, "Inhibitory effects of anethole and eugenol on the growth and toxin production of Aspergillus parasiticus", Int J Food microbiol, 10, pp.193~197 (1990)[DOI]
- Hao YY, "Inhibition of Listeria monocytogenes and Aeromonas hydrophila by plant extracts in refrigerated cooked beef", J Food Prot, 61, pp.307~312 (1998)[DOI]
- 길기정, "지유 발효추출물의 항세균 및 항산화 활성", 대한본초학회지, 30(4), pp.29~35 (2015), 대한본초학회
- 이종기, "황금탕 및 황금추출물의 식중독 세균들에 대한 생육억제 효과", 한국식품영양과학회지, 34(10), pp.1606~1610 (2005), 한국식품영양과학회
- Lee BW, "Screening of natural antimicrobial plant extract on food spoilage microorganism", Korean J Food Sci Technol, 23, pp.200~201 (1991)
- Kim KH, "Isolation and identification of antimicrobial compounds from dandelion and plantains and their effects when added to processed foodstuffs", Ph D, pp.1999
- Mvuemba HN, "Antimicrobial efficacy of cinnamon, ginger, horseradish and nutmeg extracts against spoilage pathogens", Phytoprotection, 90, pp.65~70 (2009)[DOI]
- Sharma A, "Antifungal activities of selected essential oils against Fusarium oxysporum f. sp. lycopersici 1322, with emphasis on Syzygium aromaticum essential oil", J Biosci Bioeng, 123, pp.308~313 (2017)[DOI]
- Udomlak S, "Antifungal activity of clove and cinnamon oil and their synergistic against postharvest decay fungi of grape in vitro", Nat Sci, 42, pp.169~174 (2008)