Feasibility Study on Long-Term Continuous Ethanol Production from Cassava Supernatant by Immobilized Yeast Cells in Packed Bed Reactor
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, yeast cell immobilization was carried out in a packed bed reactor (PBR) to investigate the effects of the volumetric capacity of carriers as well as the different fermentation modes on fuel ethanol production. An optimal volumetric capacity of 10 g/l was found to obtain a high cell concentration. The productivity of immobilized cell fermentation was 16% higher than that of suspended-cell fermentation in batch and it reached a higher value of 4.28 g/l/h in repeated batches. Additionally, using this method, the ethanol yield (95.88%) was found to be higher than that of other tested methods due to low concentrations of residual sugars and free cells. Continuous ethanol production using four bioreactors showed a higher productivity (9.57 g/l/h) and yield (96.96%) with an ethanol concentration of 104.65 g/l obtained from 219.42 g/l of initial total sugar at a dilution rate of 0.092 h-1. Furthermore, we reversed the substrate-feed flow directions in the in-series bioreactors to keep the cells at their highest activity and to extend the length of continuous fermentation. Our study demonstrates an effective method of ethanol production with a new immobilized approach, and that by switching the flow directions, traditional continuous fermentation can be greatly improved, which could have practical and broad implications in industrial applications.
Abstract
In this study, yeast cell immobilization was carried out in a packed bed reactor (PBR) to investigate the effects of the volumetric capacity of carriers as well as the different fermentation modes on fuel ethanol production. An optimal volumetric capacity of 10 g/l was found to obtain a high cell concentration. The productivity of immobilized cell fermentation was 16% higher than that of suspended-cell fermentation in batch and it reached a higher value of 4.28 g/l/h in repeated batches. Additionally, using this method, the ethanol yield (95.88%) was found to be higher than that of other tested methods due to low concentrations of residual sugars and free cells. Continuous ethanol production using four bioreactors showed a higher productivity (9.57 g/l/h) and yield (96.96%) with an ethanol concentration of 104.65 g/l obtained from 219.42 g/l of initial total sugar at a dilution rate of 0.092 h-1. Furthermore, we reversed the substrate-feed flow directions in the in-series bioreactors to keep the cells at their highest activity and to extend the length of continuous fermentation. Our study demonstrates an effective method of ethanol production with a new immobilized approach, and that by switching the flow directions, traditional continuous fermentation can be greatly improved, which could have practical and broad implications in industrial applications.
참고문헌 (38)
- Zhu SD, "Simultaneous saccharification and fermentation of microwave", alkali pre-treated rice straw to ethanol, 2005, pp.92, 229~225
- Baeyens J, "Challenges and opportunities in improving the production of bioethanol", Prog. Energ. Combust. Sci, 47, pp.60~88 (2015)
- Nguyen TH, "Impact of ecosystem carbon stock change on greenhouse gas emissions and carbon payback periods of cassava-based ethanol in Vietnam", Biomass Bioenerg, 100, pp.126~137 (2017)
- Najafpour G, "Ethanol production in an immobilized cell reactor using Saccharomyces cerevisiae", Bioresour. Technol, 92, pp.251~260 (2004)
- Bangrak P, "Continuous ethanol production using immobilized yeast cells entrapped in loofareinforced alginate carriers", Braz. J. Microbiol, 42, pp.676~684 (2011)
- Choi GW, "Continuous ethanol production from cassava through simultaneous saccharification and fermentation by self-flocculating yeast Saccharomyces cerevisiae CHFY0321", Appl. Biochem. Biotechnol, 160, pp.1517~1527 (2010)
- Ntihuga JN, "Efficiency of blenke cascade system for continuous bio-ethanol fermentation", Bioresour. Technol, 123, pp.221~229 (2012)
- Xu JR, "Genome sequence of the self-flocculating strain Saccharomyces cerevisiae spsc01", Genome Announc, 6(20), pp.e00367~ (2018)
- Ding WW, "Continuous ethanol fermentation in a closed circulating system using an immobilized cell coupled with PDMS membrane pervaporation", J. Chem. Technol. Biotechnol, 86, pp.82~87 (2011)
- Fan S, "Kinetic model of continuous ethanol fermentation in closed-circulating process with pervaporation membrane bioreactor by Saccharomyces cerevisiae", Bioresour. Technol, 177, pp.169~175 (2015)
- Offeman RD, "Solvent extraction of ethanol from aqueous solutions. I. Screening methodology for solvents", Ind. Eng. Chem. Res., 44, pp.6789~6796 (2005)
- Chen C, "Ethanol fermentation kinetics in a continuous and closed-circulating fermentation system with a pervaporation membrane bioreactor", Bioresour. Technol, 114, pp.707~710 (2012)
- Khongsay N, "Optimization of agitation and aeration for very high gravity ethanol fermentation from sweet sorghum juice by Saccharomyces cerevisiae using an orthogonal array design", Energies, 5, pp.561~576 (2012)
- Ajbar AH, "Study of advanced control of ethanol production through continuous fermentation", J. King Saud University-Engineering Sci, 29, pp.1~11 (2017)
- Liu QG, "Long-term production of fuel ethanol by immobilized yeast in repeated-batch simultaneous saccharification and fermentation of cassava", Energ. Fuel, 29, pp.185~190 (2015)
- Miller GL, "Use of dinitrosalicylic acid reagent for determination of reducing sugar", Anal. Chem, 31, pp.426~428 (1959)
- Chandra J, "Biofilm formation by the fungal pathogen Candida albicans : development, architecture, and drug resistance", J. Bacteriol, 183, pp.5385~5394 (2001)
- Yong Chen, "Ethanol Production by Repeated Batch and Continuous Fermentations by Saccharomyces cerevisiae Immobilized in a Fibrous Bed Bioreactor", Journal of Microbiology and Biotechnology, 23(4), pp.511~517 (2013), 한국미생물·생명공학회[DOI]
- Huang WC, "Continuous production of butanol by Clostridium acetobutylicum immobilized in a fibrousbed bioreactor", Appl. Biochem. Biotechnol, 115, pp.113~116 (2004)
- Kilonzo P, "Airlift-driven fibrous-bed bioreactor for continuous production of glucoamylase using immobilized recombinant yeast cells", J. Biotechnol, 143, pp.60~68 (2009)
- Kilonzo P, "Repeated-batch production of glucoamylase using recombinant Saccharomyces cerevisiae immobilized in a fibrous bed bioreactor", J. Ind. Microbiol. Biotechnol, 37, pp.773~783 (2010)
- Lee KH, "Enhanced production of bioethanol and ultrastructural characteristics of reused Saccharomyces cerevisiae immobilized calcium alginate beads", Bioresour. Technol, 102, pp.8191~8198 (2011)
- Deschaine BM, "Biofilm formation and toxin production provide a fitness advantage in mixed colonies of environmental yeast isolates", Ecol. Evol, 8, pp.5541~5550 (2018)
- Nikoli S, "Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var", Ellipsoideus. Biomass Bioenerg, 34, pp.1449~1456 (2010)
- Prouty AM, "Biofilm formation and interaction with the surfaces of gallstones by Salmonella spp", Infect. Immun, 70, pp.2640~2649 (2002)
- Ma Q, "OmpA influences Escherichia coli biofilm formation by repressing cellulose production through the CpxRA two-omponent system", Environ. Microbiol, 11, pp.2735~2746 (2009)
- Sutherland IW, "The biofilm matrix--an immobilized but dynamic microbial environment", Trends Microbiol, 9, pp.222~227 (2001)
- Alfattani MA, "Biofilm matrix of Candida albicans and Candida tropicalis : chemical composition and role in drug resistance", J. Med. Microbiol, 55, pp.999~1008 (2006)
- Li ZJ, "Involvement of glycolysis", gluconeogenesis and signaling regulatory pathways in Saccharomyces cerevisiae biofilms during fermentation, 2015, pp.6, 139~
- Moreno JG, "Study of the role of the covalently linked cell wall protein (Ccw14p) and yeast glycoprotein (Ygp1p) within biofilm formation in a flor yeast strain", FEMS Yeast Res., 18 (2018)[DOI]
- Trovati J, "Improving the performance of a continuous process for the production of ethanol from starch", Appl. Biochem. Biotechnol, 156, pp.506~520 (2009)
- Moona SK, "Simultaneous saccharification and continuous fermentation of sludge-containing mash for bioethanol production by Saccharomyces cerevisiae CHFY0321", J. Biotechnol, 157, pp.584~589 (2012)
- Amenaghawon NA, "Kinetic modelling of ethanol inhibition during alcohol fermentation of corn stover using Saccharomyces cerevisiae", Int. J. Appl. Eng. Res, 4, pp.798~803 (2012)
- Rittmann S, "Quantitative analysis of media dilution rate effects on Methanothermobacter marburgensis grown in continuous culture on H2 and CO2", Biomass Bioenerg, 36, pp.293~301 (2012)
- Rattanapan A, "Ethanol production by repeated batch and continuous fermentations of blackstrap molasses using immobilized yeast cells on thin-shell silk cocoons", Appl. Energ, 88, pp.4400~4440 (2011)
- Ryu DDY, "Effect of air supplement on the performance of continuous ethanol production system", Biotechnol. Bioeng, 26, pp.12~16 (1984)
- Deesuth O, "High ethanol production under optimal aeration conditions and yeast composition in a very high gravity fermentation from sweet sorghum juice by Saccharomyces cerevisiae", Ind. Crop. Prod, 92, pp.263~270 (2016)
- Xu TJ, "Continuous ethanol production using self-flocculating yeast in a cascade of fermentors", Enzyme Microb. Technol, 37, pp.634~640 (2005)