Orobol, A Derivative of Genistein, Inhibits Heat-Killed Propionibacterium acnes-Induced Inflammation in HaCaT Keratinocytes
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Acne is a chronic skin disease that typically occurs in the teens and twenties, and its symptoms vary according to age, sex, diet, and lifestyle. The condition is characterized by hyperproliferation of keratinocytes in the epidermis, sebum overproduction, excessive growth of Propionibacterium acnes, and P. acnes-induced skin inflammation. Interleukin (IL)-1α and IL-6 are predominant in the inflammatory lesions of acne vulgaris. These cytokines induce an inflammatory reaction in the skin in the presence of pathogens or stresses. Moreover, IL-1α accelerates the production of keratin 16, which is typically expressed in wounded or aberrant skin, leading to abnormalities in architecture and hyperkeratinization. Orobol (3’,4’,5,7-tetrahydroxyisoflavone) is a metabolite of genistein that inhibited the P. acnes-induced increases in IL-6 and IL-1α levels in human keratinocytes (HaCaTs) more effectively compared with salicylic acid. In addition, orobol decreased the IL-1α and IL-6 mRNA levels and inhibited the phosphorylation of inhibitor of kappa-B kinase, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, and mitogen-activated protein kinase induced by P. acnes. Finally, the expression of Ki67 was decreased by orobol. Thus, orobol ameliorated the inflammation and hyperkeratinization induced by heat-killed P. acnes and thus has potential for use in functional foods and cosmetics.
Abstract
Acne is a chronic skin disease that typically occurs in the teens and twenties, and its symptoms vary according to age, sex, diet, and lifestyle. The condition is characterized by hyperproliferation of keratinocytes in the epidermis, sebum overproduction, excessive growth of Propionibacterium acnes, and P. acnes-induced skin inflammation. Interleukin (IL)-1α and IL-6 are predominant in the inflammatory lesions of acne vulgaris. These cytokines induce an inflammatory reaction in the skin in the presence of pathogens or stresses. Moreover, IL-1α accelerates the production of keratin 16, which is typically expressed in wounded or aberrant skin, leading to abnormalities in architecture and hyperkeratinization. Orobol (3’,4’,5,7-tetrahydroxyisoflavone) is a metabolite of genistein that inhibited the P. acnes-induced increases in IL-6 and IL-1α levels in human keratinocytes (HaCaTs) more effectively compared with salicylic acid. In addition, orobol decreased the IL-1α and IL-6 mRNA levels and inhibited the phosphorylation of inhibitor of kappa-B kinase, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, and mitogen-activated protein kinase induced by P. acnes. Finally, the expression of Ki67 was decreased by orobol. Thus, orobol ameliorated the inflammation and hyperkeratinization induced by heat-killed P. acnes and thus has potential for use in functional foods and cosmetics.
참고문헌 (24)
- Cong TX, "From pathogenesis of acne vulgaris to anti-acne agents", Arch. Dermatol. Res, 311, pp.337~349 (2019)
- Samuels DV, "Acne vulgaris and risk of depression and anxiety : a meta-analytic review", J. Am. Acad. Dermatol, 83, pp.532~541 (2020)
- Kassir M, "Selective RAR agonists for acne vulgaris : a narrative review", J. Cosmet. Dermatol, 19, pp.1278~1283 (2020)
- Marron SE, "Quality-of-life research in acne vulgaris : current status and future directions", Am. J. Clin. Dermatol, 20, pp.527~538 (2019)
- Valente Duarte, "Novel pharmacological approaches for the treatment of acne vulgaris", Expert. Opin. Investig. Drugs, 23, pp.1389~1410 (2014)
- Ji J, "Correlations of SOX9 expression with serum IGF1 and inflammatory cytokines IL-1alpha and IL-6 in skin lesions of patients with acne", Euro. Rev. Med. Pharmacol. Sci, 22, pp.2549~2555 (2018)
- Ogawa R, "Keloid and hypertrophic scars are the result of chronic inflammation in the reticular dermis", Int. J. Mol. Sci, 18, pp.606~ (2017)
- Fenini G, "Potential of IL-1, IL-18 and inflammasome inhibition for the treatment of inflammatory skin diseases", Front. Pharmacol, 8, pp.278~ (2017)
- Younis S, "The interleukin-6 and interleukin-1A gene promoter polymorphism is associated with the pathogenesis of acne vulgaris", Arch. Dermatol. Res, 307, pp.365~370 (2015)
- Kim J-E, "Review of soybean phytochemicals and their bioactive properties relevant for skin health", J. Food Nut. Res, 5, pp.852~858 (2017)
- Messina M, "Soy and health update : evaluation of the clinical and epidemiologic literature", Nutrients, 8, pp.754~ (2016)
- Waqas MK, "Dermatological and cosmeceutical benefits of glycine max(soybean)and its active components", Acta Pol. Pharm, 72, pp.3~11 (2015)
- Yang H, "Orobol, an enzyme-convertible product of genistein, exerts anti-obesity effects by targeting casein kinase 1 epsilon", Sci. Rep, 9, pp.8942~ (2019)
- Dessinioti C, "Propionibacterium acnes and antimicrobial resistance in acne", Clin. Dermatol, 35, pp.163~167 (2017)
- Kiriakidis S, "Novel tempeh(fermented soyabean)isoflavones inhibit in vivo angiogenesis in the chicken chorioallantoic membrane assay", Br. J. Nutr, 93, pp.317~323 (2005)
- Lee PG, "Circular permutation of a bacterial tyrosinase enables efficient polyphenol-specific oxidation and quantitative preparation of orobol", Biotechnol. Bioeng, 116, pp.19~27 (2019)
- Lee SH, "Using tyrosinase as a monophenol monooxygenase : A combined strategy for effective inhibition of melanin formation", Biotechnol. Bioeng, 113, pp.735~743 (2016)
- Lee WP, "Inhibitory effects of amentoflavone and orobol on daclatasvirinduced resistance-associated variants of hepatitis C virus", Am. J. Chin. Med, 46, pp.835~852 (2018)
- Kolar SL, "Propionibacterium acnes–induced immunopathology correlates with health and disease association", JCI Insight, 4, pp.e124687~ (2019)
- Common JEA, "What does acne genetics teach us about disease pathogenesis? Br", J. Dermatol, 181, pp.665~676 (2019)
- Nguyen CT, "Inhibitory effects of superoxide dismutase 3 on Propionibacterium acnes-induced skin inflammation", Sci. Rep, 8, pp.4024~ (2018)
- Li X, "A review of the role of sebum in the mechanism of acne pathogenesis", J. Cosmet. Dermatol, 16, pp.168~173 (2017)
- Gabay C, "Interleukin-6 and chronic inflammation", Arthritis Res. Ther., 8(Supple 2), pp.S3~ (2006)
- Murata M, "Genistein and daidzein induce cell proliferation and their metabolites cause oxidative DNA damage in relation to isoflavone-induced cancer of estrogen-sensitive organs", Biochemistry, 43, pp.2569~2577 (2004)