The Anti-Inflammatory Effect of Trichilia martiana C. DC. in the Lipopolysaccharide- Stimulated Inflammatory Response in Macrophages and Airway Epithelial Cells and in LPS-Challenged Mice
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
A number of species of the genus Trichilia (Meliaceae) exhibit anti-inflammatory effects. However, the effect of Trichilia martiana C. DC. (TM) on lipopolysaccharide (LPS)-induced inflammation has not, to the best of our knowledge, yet been determined. Therefore, in the present study, the antiinflammatory effect of TM on LPS-stimulated RAW264.7 macrophages was evaluated. The ethanol extract of TM (TMEE) significantly inhibited LPS-induced nitric oxide (NO), prostaglandin 2 (PGE2), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). TMEE also reduced the levels of inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β and IL- 6. The upregulation of mitogen-activated protein kinases (MAPKs) and NF-κB activation was revealed to be downregulated following TMEE pretreatment. Furthermore, TMEE was indicated to lead to the nucleus translocation of nuclear factor erythroid-derived 2-related factor 2 (Nrf2) and the expression of heme oxygenase-1 (HO-1). In H292 airway epithelial cells, the pretreatment of TMEE significantly downregulated the production of LPS-stimulated IL-1β, and TMEE was indicated to increase the expression of HO-1. In animal models exhibiting LPS-induced acute lung injury (ALI), treatment with TMEE reduced the levels of macrophages influx and TNF-α production in the bronchoalveolar lavage fluid (BALF) of ALI mice. Additionally, TMEE significantly downregulated the activation of ERK, JNK and IκB, and upregulated the expression of HO-1 in the lungs of ALI mice. In conclusion, the results of the current study demonstrated that TMEE could exert a regulatory role in the prevention or treatment of the endotoxin-mediated inflammatory response.
Abstract
A number of species of the genus Trichilia (Meliaceae) exhibit anti-inflammatory effects. However, the effect of Trichilia martiana C. DC. (TM) on lipopolysaccharide (LPS)-induced inflammation has not, to the best of our knowledge, yet been determined. Therefore, in the present study, the antiinflammatory effect of TM on LPS-stimulated RAW264.7 macrophages was evaluated. The ethanol extract of TM (TMEE) significantly inhibited LPS-induced nitric oxide (NO), prostaglandin 2 (PGE2), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). TMEE also reduced the levels of inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β and IL- 6. The upregulation of mitogen-activated protein kinases (MAPKs) and NF-κB activation was revealed to be downregulated following TMEE pretreatment. Furthermore, TMEE was indicated to lead to the nucleus translocation of nuclear factor erythroid-derived 2-related factor 2 (Nrf2) and the expression of heme oxygenase-1 (HO-1). In H292 airway epithelial cells, the pretreatment of TMEE significantly downregulated the production of LPS-stimulated IL-1β, and TMEE was indicated to increase the expression of HO-1. In animal models exhibiting LPS-induced acute lung injury (ALI), treatment with TMEE reduced the levels of macrophages influx and TNF-α production in the bronchoalveolar lavage fluid (BALF) of ALI mice. Additionally, TMEE significantly downregulated the activation of ERK, JNK and IκB, and upregulated the expression of HO-1 in the lungs of ALI mice. In conclusion, the results of the current study demonstrated that TMEE could exert a regulatory role in the prevention or treatment of the endotoxin-mediated inflammatory response.
참고문헌 (35)
- Lee JW, "3, 4, 5-trihydroxycinnamic acid inhibits lipopolysaccharide(LPS)-induced inflammation by Nrf2 activation in vitro and improves survival of mice in LPS-induced endotoxemia model in vivo", Mol. Cell. Biochem, 390, pp.143~153 (2014)
- Lee JW, "Anti-inflammatory effect of stem bark of Paulownia tomentosa Steud. in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and LPS-induced murine model of acute lung injury", J. Ethnopharmacol., 210, pp.23~30 (2018)
- Jiang L, "GYY4137 attenuates LPS-induced acute lung injury via heme oxygenase-1 modulation", Pulm. Pharmacol. Ther, 54, pp.77~86 (2019)
- Zhang Y, "MCP-induced protein 1 attenuates sepsis-induced acute lung injury by modulating macrophage polarization via the JNK", c-Myc pathway, 2019, pp.75, 105741~
- Khan A, "Anomalin attenuates LPS-induced acute lungs injury through inhibition of AP-1 signaling", Int. Immunopharmacol, 73, pp.451~460 (2019)
- Gon Y, "A20 inhibits toll-like receptor 2-and 4-mediated interleukin-8 synthesis in airway epithelial cells", Am. J. Respir. Cell Mol. Biol, 31, pp.330~336 (2004)
- Liou CJ, "Water extract of Helminthostachys zeylanica attenuates LPSinduced acute lung injury in mice by modulating NF-kappaB and MAPK pathways", J. Ethnopharmacol, 199, pp.30~38 (2017)
- Lee JW, "Picrasma quassiodes (D. Don) Benn. attenuates lipopolysaccharide (LPS)-induced acute lung injury", Int. J. Mol. Med., 38, pp.834~844 (2016)
- Lim Y, "Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells", Int. J. Mol. Med., 41, pp.391~398 (2018)
- Dong ZW, "Juglanin suppresses fibrosis and inflammation response caused by LPS in acute lung injury", Int. J. Mol. Med, 41, pp.3353~3365 (2018)
- Silva JVD, "Anti-inflammatory, antioxidant and antiproliferative activities from Trichilia silvatica (C.DC)", Curr. Pharm. Biotechnol., 19, pp.973~981 (2018)
- Vicentini FA, "Treatment with Trichilia catigua ethylacetate fraction improves healing and reduces oxidative stress in TNBS-induced colitis in rats", Biomed. Pharmacother, 107, pp.194~202 (2018)
- Lee JW, "NPS2143 Inhibits MUC5AC and proinflammatory mediators in Cigarette Smoke Extract(CSE)-stimulated human airway epithelial cells", Inflammation, 40, pp.184~194 (2017)
- Lee JW, "3, 4, 5-Trihydroxycinnamic acid attenuates lipopolysaccharide(LPS)-induced acute lung injury via downregulating inflammatory molecules and upregulating HO-1", AMPK activation, 2018, pp.64, 123~130
- Kim MG, "Anti-inflammatory effects of linalool on ovalbumin-induced pulmonary inflammation", Int. Immunopharmacol, 74, pp.105706~ (2019)
- Lee JW, "Pistacia weinmannifolia ameliorates cigarette smoke and lipopolysaccharideinduced pulmonary inflammation by inhibiting interleukin8 production and NF-kappaB activation", Int. J. Mol. Med, 44, pp.949~959 (2019)
- Su K, "Chikusetsusaponin V attenuates lipopolysaccharide-induced acute lung injury in mice by modulation of the NF-kappaB and LXRalpha", Int. Immunopharmacol, 70, pp.174~179 (2019)
- Chen Y, "Glycitin alleviates lipopolysaccharide-induced acute lung injury via inhibiting NF-kappaB and MAPKs pathway activation in mice", Int. Immunopharmacol, 75, pp.105749~ (2019)
- Jiang K, "Barbaloin protects against lipopolysaccharide(LPS)-induced acute lung injury by inhibiting the ROS-mediated PI3K", AKT, Int. Immunopharmacol, pp.2018, 64[DOI]
- Qi T, "Sulforaphane exerts anti-inflammatory effects against lipopolysaccharide-induced acute lung injury in mice through the Nrf2", ARE pathway, 2016, pp.37, 182~188
- Wang X, "Anti-inflammatory effects and mechanism of the total flavonoids from Artemisia scoparia Waldst. et kit. in vitro and in vivo", Biomed. Pharmacother., 104, pp.390~403 (2018)
- Chen L, "Sonchus oleraceus Linn protects against LPS-induced sepsis and inhibits inflammatory responses in RAW264.7 cells", J. Ethnopharmacol., 236, pp.63~69 (2019)
- Su Y, "Molecular mechanism underlying anti-inflammatory activities of lirioresinol B dimethyl ether through suppression of NF-kappaB and MAPK signaling in in vitro and in vivo models", Int. Immunopharmacol., 73, pp.321~332 (2019)
- Lee JW, "NPS 2143, a selective calcium-sensing receptor antagonist inhibits lipopolysaccharide-induced pulmonary inflammation", Mol. Immunol, 90, pp.150~157 (2017)
- Guo S, "Magnoflorine ameliorates lipopolysaccharide-induced acute lung injury via suppressing NF-kappaB and MAPK activation", Front. Pharmacol, 9, pp.982~ (2018)
- Wang Y, "Sevoflurane alleviates LPS-induced acute lung injury via the microRNA27a3p", TLR4, NF-kappaB signaling pathway, pp.Int. J. Mol. Med, 2019[DOI]
- Qing R, "Cordycepin alleviates lipopolysaccharide-induced acute lung injury via Nrf2", HO-1 pathway, 2018, pp.60, 18~25
- Yin H, "Heme oxygenase-1 upregulation improves lipopolysaccharide-induced acute lung injury involving suppression of macrophage migration inhibitory factor", Mol. Immunol, 47, pp.2443~2449 (2010)
- Ren J, "Gambogic acid induces heme oxygenase-1 through Nrf2 signaling pathway and inhibits NF-kappaB and MAPK activation to reduce inflammation in LPS-activated RAW264.7 cells", Biomed. Pharmacother., 109, pp.555~562 (2019)
- Ding H, "Chicoric acid alleviates lipopolysaccharide-induced acute lung injury in mice through anti-inflammatory and anti-oxidant activities", Int. Immunopharmacol, 66, pp.169~176 (2019)
- Guo C, "Sophoraflavanone G from Sophora alopecuroides inhibits lipopolysaccharide-induced inflammation in RAW264.7 cells by targeting PI3K", Akt, JAK, HO-1 pathways, pp.Int. Immunopharmacol., 2016[DOI]
- Tran PL, "A prenylated flavonoid, 10-oxomornigrol F, exhibits antiinflammatory effects by activating the Nrf2", heme oxygenase-1 pathway in macrophage cells, 2018, pp.55, 165~173
- Min JH, "3, 4, 5-Trihydroxycinnamic acid exerts a protective effect on pulmonary inflammation in an experimental animal model of COPD", Int. Immunopharmacol, 85, pp.106656~ (2020)
- Lappalainen U, "Interleukin-1beta causes pulmonary inflammation, emphysema, and airway remodeling in the adult murine lung", Am. J. Respir. Cell Mol. Biol, 32, pp.311~318 (2005)
- Wei X, "The protective effects of Sauropus spatulifolius on acute lung injury induced by lipopolysaccharide", J. Sci. Food Agric, 98, pp.4420~4426 (2018)